Pharmacognostic and Preliminary Phytochemical Study of Ocimum gratissimum Linn. (Family: Lamiaceae)

Gupta, V. B.; Singh, Juhi; Kumar, R. Nirmal; Bhanot, Abhishek

doi:10.3923/ajps.2011.365.369

Cited by 14 publications

(9 citation statements)

References 6 publications

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“…Occimum grastissium in this study reveals abundance of glycoside and terpenoid, presence of flavonoid, saponin, phenol, alkaloid with mayer and steroids while terpenoid was absent. This work agrees with other studies [34,44,45,46] while on the contrary, [47] in his study when he screened Occimum grastissium leaves flavoniod was absent while all other compounds were present.…”

Section: Jatropha Multifida Occimum Gratissium Euphobia Hirtasupporting

confidence: 93%

Phytochemical analysis and in vitro screening of antifungal activity of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves extract

N¹,

A²,

E³

et al. 2021

GSC Biol. and Pharm. Sci.

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The research study was to understudy the qualitative and quantitative phytochemical compounds and antifungal activity of methanolic extracts of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves against 12 species of fungi. The 8 phytochemical compounds tested were saponin, phenol, tannin, flavonoids, steroids, terpenoid, alkaloid and glycoside. The antifungi sensitivity test was analysed by Kirby-bauer disc diffusion method. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) was analysed by broth tube dilution method followed by subculturing the relative samples. The plant extracts were prepared in the concentration range of 6.25, 12.5, 25, 50,100 and 200 mg/ml. The antifungal sensitivity test showed that Jatropha multifida (200mg/ml) was weakly effective against Fusarium solani and resistant against others. Occimum gratissimum, Euphorbia hirta and Mitracarpus scaber at (200mg/ml) respectively was highly effective on most of the fungi species tested with zone of inhibition of 10-20mm. Jatropha multifida had MIC and MFC (>200mg/ml), Occimum gratissimum had MIC and MFC (100 and >100mg/ml), Euphorbia hirta (25-200 and 50 - >200mg/ml) and Mitracarpus scaber had (25 - 100 and 50-200mg/ml) on most test isolates respectively. Investigations showed, Mitracarpus scaber were highly effective, followed by Euphorbia hirta, Occimum gratissimum and Jatropha multifida on the test organisms and could be attributed to the presence of phytochemical compounds and as such can be harnessed and used as a source of alternative medicine especially in the tropics like the South-eastern Nigeria.

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Section: Jatropha Multifida Occimum Gratissium Euphobia Hirtasupporting

confidence: 93%

Phytochemical analysis and in vitro screening of antifungal activity of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves extract

N¹,

A²,

E³

et al. 2021

GSC Biol. and Pharm. Sci.

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“…This study investigated the sub-chronic effects of oral administration of butanolic and ethyl acetate fractions from methanolic leaf extract of Ocimum gratissimum on reproductive hormones, sperm parameters and testicular histology as indices for fertility in male Wistar rats. The percentage yield of the methanolic extract of Ocimum gratissimum was determined to be 10.94 percent in contrast to 8.88 percent and 9.60 percent earlier reported [13] and [36] respectively, and this increase might be as a result of the dual maceration technique employed for the leaf extraction.…”

Section: Discussioncontrasting

confidence: 62%

“…Ocimum gratissimum, commonly referred to as ‘scent leaves’, and also known as Ramtulsi in Hindi, Bengali, and Gujrati; Fever leaf in English; Banjere in Punjabi; Rama tulsi in Malyalam; Elumicham tulasi in Tamil; Nimma tulsi in Kannad [13], is a shrub of the Lamicaea family of plants [14], genus Ocimum and species gratissimum [15] which is native to Central Africa and Southeast Asia [16] but cultivated in Nigeria, Southern American and Asian countries [13], is one of the common herbs consumed in many part of the world because of its palatability and claimed medicinal benefits. It is known locally in Nigeria as Daidoya, Efinrin and Nchoanwu among the Hausa, Yoruba and Ibo ethnic groups respectively [17] and employed locally as component in herbal preparations [18].…”

Section: Introductionmentioning

confidence: 99%

Safety effect of fractions from methanolic leaf extract of Ocimum gratissimum on reproduction in male wistar rats

et al. 2019

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“…gratissimum, O. sanctum and O. americanum, and reported different oil content in them which was also subjected to GC and TLC profiling. Similar investigation had been reported the variations in the morphological characters to be useful in identification and evaluation of the plant (Choudhury et al 2011, Gupta et al 2011. Tewari et al (2012) reported an Ocimum species, O. kilimandscharicum from western Himalayan region and evaluated certain nutritional characters among its population of O. kilimandscharicum.…”

Section: Introductionmentioning

confidence: 93%

Population Polymorphism Study Among Tulsi (Ocimum sp.) Ecotypes In Assam, India Using Morphological And Biochemical Parameters

Borah

Chutia

Modi³

et al. 2015

International Journal of Crop Science and Technology

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The genus Ocimum, (family Lamiaceae), displays great variability. Prevalence of cross pollination, polyploidy and interspecific hybridization has complicated its taxonomy, making its systematic study difficult. This study investigated the morphological and biochemical variability among 18 ecotypes collected from different parts of the Assam state, India. Considerable diversity was found using all the approaches. The quantitative morphological parameters showed wide variability, not always co-relating with their geographical distances. The Euclidean distance ranged between 6.06 (between the closest accessions) and 59.00 (between the most distant accessions). Phylogenetic analysis divided the accessions into two major clusters sharing approx. 76% identity, and a minor cluster (with three accessions) sharing only approx. 65% identity with the other two. A great deal of diversity was found in the qualitative characters too; that divided the ecotypes into two to five groups. The biochemical characters also revealed wide polymorphism; the distance varied from 1.690 to 58.574. Similar to the morphological characters, the biochemical parameters too did not always correlate with geographical distances. The phylogenetic mapping divided the accessions into two major clusters, sharing approx. 80% similarity. Further work will be needed to correlate the distance based on the morphological and the biochemical characters. (Omidbaigi et al. 2010, Carovic-Stanko et al. 2011. However, there is little information available regarding the plant's diversity in India and in Asia; there is confusion regarding even its genome structure. The northeastern part of India, including the state of Assam, being a biodiversity hotspot, would be a good start point to study the diversity of the plant in the country as well as in the region. To our knowledge, this is the first report on the categorization of the morphological and biochemical parameters of the plant. MATERIALS AND METHODS Plant Materials and Growth ConditionsThe plant materials were collected from 18 different districts of Assam and were named as accession (Acc) #1 to accession #18 (Table 1; Fig 1). Seeds were also collected for all the ecotypes, and were germinated in laboratory on moist filter paper in Assam Agricultural University (AAU), Jorhat. The seedlings were grown in the potting mixture prepared by mixing soil, sand and dried manure in the ratio of 2:1:1 v/v, and the plants were maintained in typical greenhouse conditions (16/8 hours of light/dark cycle and 22-25°C temperature) in the AAU facility. The morphological data were recorded at the vegetative (150 day-old) stage of the plants for the leaf and the stem characters; the inflorescence characters were taken at the reproductive stage, at the 200 day-old plants. For collection of the biochemical data, leaf samples were collected at the same stage and were processed for isolation and/ or quantification of the parameters. Morphological ParametersDefined descriptors are not available in tulsi. Therefore, based on t...

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Pharmacognostic and Preliminary Phytochemical Study of Ocimum gratissimum Linn. (Family: Lamiaceae)

Cited by 14 publications

References 6 publications

Phytochemical analysis and in vitro screening of antifungal activity of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves extract

Phytochemical analysis and in vitro screening of antifungal activity of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves extract

Safety effect of fractions from methanolic leaf extract of Ocimum gratissimum on reproduction in male wistar rats

Population Polymorphism Study Among Tulsi (Ocimum sp.) Ecotypes In Assam, India Using Morphological And Biochemical Parameters

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