Phallacidin stains the kinetochore region in the mitotic spindle of the green algaeOedogonium spp.

Abstract

ErratumIn the article "Phallacidin stains the kinetochore region in the mitotic spindle of the green algae Oedogonium spp." by Sampson and Pickett-Heaps (Protoplasma 217: 166-176, 2001) the collective legends for Figs. 1-6 and 8-13 on pages 169 and 171 were incorrect and should read:

Mitosis: spindle evolution and the matrix model

“…Actin and myosin in the spindle. We and our colleagues have intensively investigated whether actin and myosin could be involved in mitosis (Forer, 1988;Czaban and Forer, 1994;Sampson et al, 1996;Forer and Pickett-Heaps, 1998;Sampson and Pickett-Heaps, 2001;Forer et al, 2003;Forer, 2005, 2007;Fabian et al, 2007a;Forer et al, 2007bForer et al, , 2008. In brief, actin, myosin and other "muscle" proteins are present in spindles (e.g., Fig.…”

Phallacidin stains the mitotic spindle of the diatom Pinnularia spp.

“…As previously discussed (Forer and Pickett-Heaps, 1998;Sampson and Pickett-Heaps, 2001;Sampson et al, 1996), actin is generally considered to be a nonfunctional component of mitotic spindles. However, a number of EM studies show microfilaments amongst the spindle MTs of higher plant (e.g., Forer et al, 1979;Schmit and Lambert, 1988), animal (e.g., Euteneuer et al, 1977;Gabrion et al, 1980) and algal (Pickett-Heaps and Carpenter, 1993) cells.…”

“…However, a number of EM studies show microfilaments amongst the spindle MTs of higher plant (e.g., Forer et al, 1979;Schmit and Lambert, 1988), animal (e.g., Euteneuer et al, 1977;Gabrion et al, 1980) and algal (Pickett-Heaps and Carpenter, 1993) cells. Fluorescently labelled phallotoxins have shown actin within spindles of plant sporocytes, plant tissue culture cells and algal cells (Sampson and Pickett-Heaps, 2001). A prominent feature of the majority of these studies is the close association observed between microfilaments and KMTs (Sampson and Pickett-Heaps, 2001).…”

“…Fluorescently labelled phallotoxins have shown actin within spindles of plant sporocytes, plant tissue culture cells and algal cells (Sampson and Pickett-Heaps, 2001). A prominent feature of the majority of these studies is the close association observed between microfilaments and KMTs (Sampson and Pickett-Heaps, 2001). Various observations have suggested that the actin and MT systems can be linked both physically and functionally (e.g., Ahmad et al, 2000;Collings et al, 1996;Langford, 2002;Salitz and Schmitz, 1989;Uyeda and Furuya, 1989;Waterman-Storer and Salmon, 1997), and numerous proteins are capable of interacting with both (Goode et al, 2000).…”

“…We previously showed, via fluorescence labelling, that actin is present in Oedogonium spp. spindles in the position in which Schibler and Pickett-Heaps saw filaments (Sampson and Pickett-Heaps, 2001). Actin is important for mitosis in Oedogoniumspp.…”