Phagocytosis of spermatozoa and latex beads by the epithelial cell of the cat oviduct: Combined SEM and TEM study.

Murakami, Masahirô; Nishida, Tadashi; Shiromoto, Mitsuru; Iwanaga, Shigeaki

doi:10.1679/aohc.48.519

Cited by 15 publications

(7 citation statements)

References 11 publications

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“…Sperm adherence results in prolonged viability. Perhaps carbohydrates in salamander spermathecal secretions play a similar role, but phagocytosis, which is also well documented in the mammalian oviduct (Murakami et al . 1985), results when sperm become enmeshed (pushed?)…”

Section: Discussionmentioning

confidence: 78%

Sperm aggregations in the spermatheca of the red back salamander (Plethodon cinereus)

Sever

Siegel

2006

Acta Zoologica

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 Abstract Sever, D. M. and Siegel, D. S. 2006. Sperm aggregations in the spermatheca of the red back salamander ( Plethodon cinereus ). -Acta Zoologica (Stockholm) 87 : 331-340The spermatheca of Plethodon cinereus is a compound tubular gland that stores sperm from mating in early spring (March-April) to oviposition in summer (June-July). The seasonal variation of sperm storage in this species has previously been studied by light and transmission electron microscopy. In this paper, sperm aggregations, interaction of sperm with the spermathecal epithelium, and spermathecal secretions are studied using scanning electron microscopy. Within spermathecal tubules, relatively small groups of sperm are aligned along their entire lengths in parallel arrays. This pattern is similar to other plethdontids with complex spermathecae. Lumina of spermathecal tubules are filled with secretory material in April prior to the arrival of sperm, and after sperm appear, a coating of secretory material persists on the apices of the spermathecal epithelium. Sperm peripheral to the central luminal mass can become embedded in the secretory matrix or pushed deeper into the spermathecal epithelium. The spermathecal secretions may serve to attract and prolong the viability of sperm, but sperm that become enmeshed in the secretions or epithelium are phagocytized. Sperm and spermathecal secretions are largely absent after ovulation and in summer months, and new secretory vacuoles are formed in fall, although mating does not occur until spring.

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Section: Discussionmentioning

confidence: 78%

Sperm aggregations in the spermatheca of the red back salamander (Plethodon cinereus)

Sever

Siegel

2006

Acta Zoologica

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“…Spermatozoa are able to activate neutrophils 12 , which are able to trigger the phagocytosis of these male gametes and hinder their motility 9, 12 . Alternatively, many of mammalian sperm are attacked and phagocytosed by leukocytes and epithelial cells in the FRT 14–16 . Isthmic epithelial cells and leukocytes are believed to involve in phagocytosis of surplus/post-capacitated sperm that entered the oviduct and did not contribute in fertilization 15 , implying a physiological role for spermophagy process in regulating possible sever inflammation that may be resulted from dead/remained post-capacitated sperm 15, 16 .…”

Section: Discussionmentioning

confidence: 99%

“…Since spermatozoa are able to activate neutrophils 12 , it seems that sperm can be eliminated from the FRT through phagocytosis by PMNs. It has been shown that in humans 13 , cats 14 , and mice 15 the remained/post-fertilization sperm cells in the oviduct are phagocytosed by isthmic epithelial cells and leukocytes 15, 16 . Indeed, post-capacitated sperm cells which lost their fertilizability have been shown to induce migration of leukocytes into the FRT, undergo phagocytosis and, thereby, are eliminated from the FRT 16 .…”

Section: Introductionmentioning

confidence: 99%

An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle

Kowsar

Keshtegar

Marey

et al. 2017

Sci Rep

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After intercourse/insemination, large numbers of sperm are deposited in the female reproductive tract (FRT), triggering a massive recruitment of neutrophils (PMNs) into the FRT, possibly to eliminate excessive sperm via phagocytosis. Some bovine oviductal fluid components (BOFCs) have been shown to regulate in vitro sperm phagocytosis (spermophagy) by PMNs. The modeling approach-based logistic regression (LR) and autoregressive logistic regression (ALR) can be used to predict the behavior of complex biological systems. We, first, compared the LR and ALR models using in vitro data to find which of them provides a better prediction of in vitro spermophagy in bovine. Then, the best model was used to identify and classify the reciprocal effects of BOFCs in regulating spermophagy. The ALR model was calibrated using an iterative procedure with a dynamical search direction. The superoxide production data were used to illustrate the accuracy in validating logit model-based ALR and LR. The ALR model was more accurate than the LR model. Based on in vitro data, the ALR predicted that the regulation of spermophagy by PMNs in bovine oviduct is more sensitive to alpha-1 acid glycoprotein (AGP), PGE2, bovine serum albumin (BSA), and to the combination of AGP or BSA with other BOFCs.

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“…Sperm viability until ovulation is manifested by prolonged motility and fertility (Talevi & Gualtieri 2004, Chang & Suarez 2012. It has been shown that in humans (Haney et al 1983), cats (Murakami et al 1985), and mice (Chakraborty & Nelson 1975) the superfluous sperm that remain in the oviduct after ovulation are eliminated through phagocytosis by epithelial and immune cells. However, in cows, the presence of such immune cells, their interaction with stored sperm, and the possible regulators of the local immune microenvironment in the oviduct have not been investigated yet.…”

Section: Introductionmentioning

confidence: 99%

Bovine oviduct epithelial cells downregulate phagocytosis of sperm by neutrophils: prostaglandin E2 as a major physiological regulator

Marey¹,

Liu²,

Kowsar³

et al. 2014

Reproduction

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This study aimed to investigate the presence of polymorphonuclear neutrophils (PMNs) in bovine oviduct fluid under physiological conditions and to determine the possible role of bovine oviduct epithelial cells (BOECs) in the regulation of the phagocytic activity of PMNs for sperm. During the pre-ovulatory stage, PMNs were identified in the bovine oviduct fluid in relatively constant numbers. In our experiments, PMNs were incubated for 4 h with the supernatant of cultured BOECs stimulated for 24 h by LH (10 ng/ml). Phagocytosis was then assayed by co-incubation of these PMNs with sperm treated to induce capacitation. The BOEC supernatant significantly suppressed sperm phagocytosis by PMNs, and the LH-stimulated BOEC supernatant further suppressed phagocytosis. Importantly, in the BOEC culture, LH stimulated the secretion of prostaglandin E 2 (PGE 2 ), which dose-dependently (10 K6 , 10 K7 , and 10 K8 M) suppressed sperm phagocytosis by PMNs. Furthermore, a PGEP 2 receptor antagonist significantly abrogated the inhibition of phagocytosis by the LH-stimulated BOEC supernatant. Additionally, using scanning electron microscopy, incubation of PMNs with either PGE 2 or LH-stimulated BOEC supernatant before phagocytosis was found to prevent the formation of DNA-based neutrophil extracellular traps for sperm entanglement. The results indicate that sperm are exposed to PMNs in the oviduct and PGE 2 released into the oviduct fluid after LH stimulation may play a major role in the suppression of the phagocytic activity of PMNs for sperm via interaction with EP 2 receptors. Thus, the bovine oviduct provides a PGE 2 -rich microenvironment to protect sperm from phagocytosis by PMNs, thereby supporting sperm survival in the oviduct.Free Japanese abstract A Japanese translation of this abstract is freely available at

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Phagocytosis of spermatozoa and latex beads by the epithelial cell of the cat oviduct: Combined SEM and TEM study.

Cited by 15 publications

References 11 publications

Sperm aggregations in the spermatheca of the red back salamander (Plethodon cinereus)

Sperm aggregations in the spermatheca of the red back salamander (Plethodon cinereus)

An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle

Bovine oviduct epithelial cells downregulate phagocytosis of sperm by neutrophils: prostaglandin E2 as a major physiological regulator

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