Phagocytosis of human blood leukocytes: A simple micromethod

Větvička, Václav; Fornůsek, L; Kopeček, Jindřich; Kamínková, J; Kaspárek, L; Vránová, M

doi:10.1016/0165-2478(82)90040-2

Cited by 102 publications

(55 citation statements)

References 7 publications

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“…Phagocytic activity of phagocytes was tested according to Vìtvièka et al (1982) by using microspheric hydrophilic metacrylate particles (MSHP) method and the results given as phagocytic activity index. Lymphocyte blastogenesis was tested by the fluorescence assay with ethidium bromide (Nagahata et al, 1986) and results are expressed as stimulation index.…”

Section: Resultsmentioning

confidence: 99%

Effect of vitamin E and selenium on blood glutathione peroxidase activity and some immunological parameters in sheep

Milad¹,

Kováč²,

Rácz³

et al. 2001

Vet. Med. - Czech

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The objective of this study was to determine the effect of vitamin E and selenium administration on glutathione peroxidase (GSH-Px) activity and selected parameters of cellular immunity (metabolic activity, phagocytic activity, lymphocyte blastogenic response). Nine pregnant sheep with body weight of 42 to 66 kg were divided into two groups. Before lambing, the first group (n = 5) was given subcutaneously 5 mg vitamin E and 0.4 mg selenium per kg body weight while the second group (n = 4) was given no treatment and served as control. Blood samples from all sheep were collected before the treatment, 14 days after lambing and 30 days after lambing. Whole blood GSH-Px activities were significantly higher in the vitamin E and selenium treated sheep than in the control in the samples taken 14 days after lambing and 30 days after lambing (P < 0.01; P < 0.001, respectively). The evaluation of immunological parameters showed declines in immunological parameters measured on days 14 and 30 after lambing. The administered preparation led to significant effects (P < 0.001; P < 0.05) on phagocytic activity index of leukocytes and phagocytic activity index of neutrophils, respectively.

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Section: Resultsmentioning

confidence: 99%

Effect of vitamin E and selenium on blood glutathione peroxidase activity and some immunological parameters in sheep

Milad¹,

Kováč²,

Rácz³

et al. 2001

Vet. Med. - Czech

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“…Ingestion of MSHP particles by polymorphonuclear cells (PMNs) was determined by a modified test described by Vetvicka et al (1982). One-hundred cells were examined to determine the relative number of white cells containing at least three engulfed particles (phagocytic activity) and the index of phagocytic activity (number of engulfed particles/number of neutrophils and monocytes).…”

Section: Sample Analysismentioning

confidence: 99%

Effect of Cinnamomum zeylanicum Essential Oil on Antioxidative Status in Broiler Chickens

et al. 2009

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The experiment was conducted to investigate the effects of Cinnamomum zeylanicum essential oil on antioxidant status of chickens. Thirty-two female Ross 308 hybrid broilers were fed one of four diets supplemented with 0%, 0.1%, 0.05% and 0.025% of essential oil for 38 days. Blood, liver, kidney and duodenal epithelium were collected for the subsequent evaluation of antioxidant status. Feeding of adiet supplemented with 0.1% of essential oil significantly decreased the concentration of malondialdehyde (MDA) in plasma and duodenal mucosa in comparison with the control group (0%). The activities of glutathione peroxidase (GPx) were significantly higher in blood of chicks fed the diet containing 0.1% of essential oil. Diets containing 0.05% and 0.025% of essential oil reduced alanine amino transferase (ALT) activity in plasma in comparison with the control group. Blood phagocytic activity significantly increased in chickens fed the diet supplemented with 0.1% and the index of phygocytic activity was affected by the diet containing 0.025% of essential oil in comparison with the control group. The present investigation shows that Cinnamomum zeylanicum essential oil exhibits a significant antioxidant activity in fattening chickens and can be used as a source of antioxidant in dietary supplement.

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“…Ingestion of MSHP by polymorphonuclear cells (PMN) was determined using a modified test described by Vetvicka et al (1982): 50 μl of MSHP suspension (ARTIM, Prague, Czech Republic) was mixed with 100 μl of blood in an Eppendorf-type test tube and incubated at 37 o C for 1 h. Blood smears were then prepared and stained in accordance with May-Grunwald and Giemsa--Romanowski (Pappenheim stain -May-Grü nwald solution was added on a slide with blood smear and worked for 3 minutes. Then 5 ml of distilled water was added and worked for 1 minute.…”

Section: Biochemical Parameters and Phagocytic Activitymentioning

confidence: 99%

Effect of combined administration of enterocin 4231 and sage in rabbits

Szabóová¹,

Lauková²,

Chrastinová³

et al. 2011

Polish Journal of Veterinary Sciences

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Enterocin (Ent) 4231, produced by non-rabbit origin strain Enterococcus faecium CCM 4231 was used in combination with sage plant extract in rabbits with the aim to check their antimicrobial activity against microbiota, their effect on immunological, biochemical blood parameters, values of volatile fatty acids in caecum, Eimeria sp. oocysts occurrence and selected parameters of rabbits meat. The animals were divided into three experimental groups (EG1-Ent 4231; EG2-sage; EG3-Ent 4231 with sage) and control group (CG); 24 rabbits in each. Natural substances (NS) were administered for 21 days. The experiment lasted for 42 days. The reduction of microbiota in faeces was observed in EG3 at day 21 by a decrease in the numer of coagulase-positive staphylococci (P<0.01) in comparison with that determined in CG. The bacterial counts in the caecum were lower than those found in faeces. A decrease in the numer of Pseudomonas-like sp. in caeca of the experimental groups was observed at days 21 and 42 (difference in range 0.40-1.87 log cycles) comparing with that determined in CG. At day 21, a significant increase in phagocytic activity (PA, P<0.001) was found in blood of rabbits from EG2 comparing with that observed in CG. At day 42, a significant increase in PA (P<0.001) was determined in all experimental groups in comparison with CG. At day 21, in caecal content of EG3 significantly higher values of lactic acid were observed (P<0.05) in comparison with those found in CG. The reduction of Eimeria sp. oocysts was demonstrated after application of each of NS. Addition of NS did not influence biochemical parameters, meat quality of the animals and does not influence negatively the health status of rabbits.

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Phagocytosis of human blood leukocytes: A simple micromethod

Cited by 102 publications

References 7 publications

Effect of vitamin E and selenium on blood glutathione peroxidase activity and some immunological parameters in sheep

Effect of vitamin E and selenium on blood glutathione peroxidase activity and some immunological parameters in sheep

Effect of Cinnamomum zeylanicum Essential Oil on Antioxidative Status in Broiler Chickens

Effect of combined administration of enterocin 4231 and sage in rabbits

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