Phagocytosis of Bovine Blood and Milk Polymorphonuclear Leukocytes after Ozone Gas Administration In Vitro

Ducusin, Rio John T.; Nishimura, Masakazu; Sarashina, Takao; Uzuka, Yuji; Tanabe, Shigeyuki; Otani, Masayuki

doi:10.1292/jvms.65.535

Cited by 16 publications

(15 citation statements)

References 18 publications

(23 reference statements)

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“…Host response to biofilm plays a critical role in the pathogenesis of periodontitis [47]; many proinflammatory cytokines are involved in inflammation and bone loss [48]. Resolution of inflammation may be supported by ozone; ozone was found to inhibit NFκB-pathways [49] as well to increase the phagocytotic capacity of bovine PMNs in inflammation [50]. Ozone was shown to be toxic to human oral epithelial cells and gingival fibroblasts but in a lower extent than different antimicrobials [51].…”

Section: Discussionmentioning

confidence: 99%

Effect of ozone on periodontopathogenic species—an in vitro study

2011

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The in vitro study was aimed to determine the effect of ozone on periodontopathogenic microorganisms. Ozone was generated for 6 s-2 × 24 s (corresponding to 0.56 mg-2 × 2.24 mg of ozone) against 23 mainly anaerobic periodontopathogenic species. Agar diffusion test was used as a screening method. Then, the killing activity was tested in a serum-free environment and with 25% v/v inactivated serum. Further, the effect of ozone on bactericidal activity of native serum was analyzed against Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. Agar diffusion test showed a high efficacy of ozone against microorganisms, especially against Porphyromonas gingivalis. This result was confirmed by the killing tests; most of the strains in a concentration of 10(5) were completely eliminated after twofold 18-s application of ozone. Only four of the six potentially "superinfecting" species (Staphylococcus aureus, Enterococcus faecalis, Enterobacter cloacae, Candida albicans) survived in part. Addition of heat-inactivated serum reduced the killing rate of ozone by 78% after 6-s and by 47% after twofold 18-s exposures; no strain was completely eradicated after any application of ozone. The bactericidal effect of native serum was enhanced after application of ozone; no effect was visible on the included A. actinomycetemcomitans strain which was found to be completely resistant to the bactericidal action of serum. In conclusion, (a) ozone has a strong antibacterial activity against putative periodontopathogenic microorganisms, and (b) the bactericidal effect is reduced in the presence of serum. Ozone may have potential as an adjunctive application to mechanical treatment in periodontitis patients.

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Section: Discussionmentioning

confidence: 99%

Effect of ozone on periodontopathogenic species—an in vitro study

2011

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“…The high cure rate of acute mastitis in dairy cows is attributable to ozone's characteristic feature of inducing less release of endotoxin. The effects of ozone on immunity have been reported [2,18,19]; ozone may downregulate the cytotoxic activity of immunocytes.…”

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confidence: 99%

Comparison of the Amounts of Endotoxin Released from Escherichia coli after Exposure to Antibiotics and Ozone: an In Vitro Evaluation

Shinozuka

Uematsu

Takagi

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Antibiotic therapy administered for treatment of gram-negative bacterial infections can exert a negative effect in the form of endotoxin shock. Therefore, a sterilization method that releases little endotoxin is required. This in vitro study aimed to compare the amounts of endotoxin released from Escherichia coli exposed to antibiotics (aminobenzylpenicillin, kanamycin, oxytetracycline, sulfadimethoxine and enrofloxacin) or ozone. The bacterial growth and amount of endotoxin released were measured at specific time points. Compared with antibiotic treatment, ozone sterilization induced release of smaller amounts of endotoxin from the bacteria. Moreover, no major differences were observed when ozone was used against the purified endotoxin. We conclude that compared with antibiotic treatment, ozone sterilization may release smaller amounts of endotoxin. KEY WORDS: Escherichia coli, endotoxin release, ozone.

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“…It was reported that treatment with ozone released fewer endotoxins in the milk than treatment with antibiotics; further, no iatrogenic endotoxic shock occurred as an adverse effect, unlike that in the case of antibiotic treatment [10]. In addition, it has been reported that ozone may result in the downregulation of the cytotoxic activity of immunocytes [1,21,22]. Therefore, we speculated that using ozone water as an irrigation fluid during MIR as the first line of treatment for coliform mastitis was possible to reduce mortality.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Efficacy of Mammary Irrigation Regimen in Dairy Cattle Diagnosed with Acute Coliform Mastitis

Shinozuka

Hirata²,

Ishibashi³

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. The objective of this field study was to determine the therapeutic efficacy of mammary irrigation for the treatment of dairy cattle diagnosed with acute coliform mastitis caused by gram-negative bacteria. Additionally, the effects of different mammary irrigation regimen fluids such as ozone water and normal saline were compared. Dairy cattle clinically diagnosed with acute coliform mastitis (n=57) were enrolled in the study, randomly assigned to 1 of 3 groups, and received the following treatments: systemic antibiotic administration (SAA group; n=40), mammary irrigation regimen (MIR group; n=10), and both treatments (MIX group; n=7). Significant antipyretic effects, as assessed by rectal temperature measurement, were observed in the MIX and MIR groups. Although 2 irrigating fluids were used, namely, ozone water and normal saline, no significant difference was observed between the 2 groups. Fourteen days after the onset of the treatments, the milk yield recovery rate in MIR group tended to be higher (p=0.06) than that in the SAA group. Additionally, after 30 days of treatment, the MIR group cows demonstrated significantly higher successful recovery rates (p<0.05) than the SAA group cows. These results indicate that mammary irrigation with normal saline is an effective treatment for acute coliform mastitis in dairy cattle.

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Phagocytosis of Bovine Blood and Milk Polymorphonuclear Leukocytes after Ozone Gas Administration In Vitro

Cited by 16 publications

References 18 publications

Effect of ozone on periodontopathogenic species—an in vitro study

Effect of ozone on periodontopathogenic species—an in vitro study

Comparison of the Amounts of Endotoxin Released from Escherichia coli after Exposure to Antibiotics and Ozone: an In Vitro Evaluation

Therapeutic Efficacy of Mammary Irrigation Regimen in Dairy Cattle Diagnosed with Acute Coliform Mastitis

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