Effect of ozone on periodontopathogenic species—an in vitro study

Abstract

The in vitro study was aimed to determine the effect of ozone on periodontopathogenic microorganisms. Ozone was generated for 6 s-2 × 24 s (corresponding to 0.56 mg-2 × 2.24 mg of ozone) against 23 mainly anaerobic periodontopathogenic species. Agar diffusion test was used as a screening method. Then, the killing activity was tested in a serum-free environment and with 25% v/v inactivated serum. Further, the effect of ozone on bactericidal activity of native serum was analyzed against Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. Agar diffusion test showed a high efficacy of ozone against microorganisms, especially against Porphyromonas gingivalis. This result was confirmed by the killing tests; most of the strains in a concentration of 10(5) were completely eliminated after twofold 18-s application of ozone. Only four of the six potentially "superinfecting" species (Staphylococcus aureus, Enterococcus faecalis, Enterobacter cloacae, Candida albicans) survived in part. Addition of heat-inactivated serum reduced the killing rate of ozone by 78% after 6-s and by 47% after twofold 18-s exposures; no strain was completely eradicated after any application of ozone. The bactericidal effect of native serum was enhanced after application of ozone; no effect was visible on the included A. actinomycetemcomitans strain which was found to be completely resistant to the bactericidal action of serum. In conclusion, (a) ozone has a strong antibacterial activity against putative periodontopathogenic microorganisms, and (b) the bactericidal effect is reduced in the presence of serum. Ozone may have potential as an adjunctive application to mechanical treatment in periodontitis patients.

Antimicrobial Effect of Ozone Made by KP Syringe of HighFrequency Ozone Generator

“…Similar results were obtained by Eik et al (25). They studied the effect of ozone on periodontopathogenic species and noticed that four potentially "superinfecting"species,…”

Ozonated saline shows activity against planktonic and biofilm growing Staphylococcus aureus in vitro: a potential irrigant for infected wounds

“…These antiseptic agents also show significant antibacterial activity, at least against planktonic bacteria; however, ozone is expected to offer certain advantages such as low cytotoxicity and rapid quenching in the surgical wound. Previously, ozonated fluids have been shown to display antibacterial activity against a wide range of microorganisms including Escherichia coli , Enterococcus faecalis , Listeria spp. , and S. aureus .…”

Ozone Therapy in the Treatment of Periodontal Disease

“…Thus, applications of medical ozone may provide a potential benefit in the treatment of periodontal diseases by reducing and eradicating subgingival periodontopathogenic species in inaccessible sites. [4][5][6][7][8][9][10][11] Applications of medical ozone are a new treatment modality that has been developing rapidly carbonhydrates,proteins, lipids) etc. [12][13][14][15][16] While ozone has been evaluated by many clinical studies, the recent studies have reported controversial results about the benefits of its for periodontal treatment.…”

“…[12][13][14][15][16] While ozone has been evaluated by many clinical studies, the recent studies have reported controversial results about the benefits of its for periodontal treatment. Some current studies showed that a number of the periodontal pathogens are susceptible to medical ozone, suggesting that it is advantageous for conventional periodontal therapy [4][5][6][7][8][9][10][11] , whereas others reported that adjunctive use of ozone showed no significant benefits. [17][18][19][20] The some systematic reviews conclude that the inclusion of the ozone as an adjunct to conventional periodontal treatment seems to be therapeutically useful.…”