Phagocytosis and nitric oxide production by peritoneal adherent cells in response to Candida albicans in aging: a collaboration to elucidate the pathogenesis of denture stomatitis

Gardizani, Taiane Priscila; Pinke, Karen Henriette; Lima, Heliton Gustavo de; Lara, Vanessa Soares

doi:10.1590/1678-7757-2016-0322

Cited by 3 publications

(1 citation statement)

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“…Nitric oxide (NO) release by BMMCs was determined by the total amount of nitrite (NO 2 ‐ ) in the supernatant using the Griess method 30 . The quantification of intracellular NO was performed as described previously 31 .…”

Section: Methodsmentioning

confidence: 99%

Mast cells exhibit intracellular microbicidal activity against Aggregatibacter actinomycetemcomitans

Lima

Pinke

Lopes

et al. 2020

J of Periodontal Research

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Background and Objective Several studies have demonstrated that mast cells are equipped with versatile tools to combat and kill bacteria. Additionally, mast cells produce and secrete a variety of mediators, which either regulate the host's immune system or directly attack bacteria. In this study, the intracellular microbicidal capacity of mast cells against Aggregatibacter actinomycetemcomitans was evaluated. Methods Murine mast cells were challenged in vitro with A actinomycetemcomitans for 3, 5, 10, and 24 hours. Subsequently, the colony‐forming units were counted. Additionally, the production and release of nitric oxide and hydrogen peroxide were analyzed by DAF‐FM diacetate, the Griess reaction, and the Amplex Red kit, respectively. Cell death was evaluated using FITC Annexin V and propidium iodide staining. Results Mast cells are able to efficiently eliminate periodontopathogen, with best results after 10 hours of intracellular challenge. The production/release of nitric oxide—and to a lesser extent of hydrogen peroxide—by mast cells was in agreement with its microbicidal capacity. Ninety percent of the mast cells maintained their cellular viability even after 24 hours of bacterial challenge. Conclusions This is—to the best of our knowledge—the first report to describe the intracellular microbicidal activity of mast cells against A actinomycetemcomitans, concerning the production and release of potentially bactericidal substances. Further, the low number of cell deaths confirms that the decreased number of colony‐forming units was due to the higher antimicrobial activity of mast cells. The results highlight the importance of these cells in the defense mechanisms of biofilm‐induced periodontal disease.

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Section: Methodsmentioning

confidence: 99%