Phagocytic Response of Rat Liver Capillary Endothelial Cells and Kupffer Cells to Positive and Negative Charged Iron Colloid Particles

Seno, Satimaru; Tanaka, Akisuke; Urata, Masako; Hirata, Koichi; Nakatsuka, Hisashi; Yamamoto, Shinsuke

doi:10.1247/csf.1.119

Cited by 17 publications

(6 citation statements)

References 10 publications

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“…Data referring to the anionic material uptake in the liver are contradictory . While the native ferritin uptake was attributed to the parenchimal cells only (21), the sinusoidal macrophages (Kupffer cells) were reported to be responsible for the ingestion of negatively charged colloidal carbon particles (15). At least for the time intervals used in our experiments, the hepatocytes did not bind or internalize any of the anionic markers .…”

Section: Discussionmentioning

confidence: 59%

Surface charge distribution on the endothelial cell of liver sinusoids.

Ghitescu¹,

Fixman²

1984

The Journal of Cell Biology

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The topography of the charged residues on the endothelial cell surface of liver sinusoid capillaries was investigated by using electron microscopic tracers of different size and charge. The tracers used were native ferritin (pl 4 .2-4.7) and its cationized (pl 8.4) and anionized (pl 3 .7) derivatives, BSA coupled to colloidal gold (pl of the complex 5.1), hemeundecapeptide (pl 4.85), and alcian blue (pl >10) . The tracers were either injected in vivo or perfused in situ through the portal vein of the mouse liver. In some experiments, two tracers of opposite charge were sequentially perfused with extensive washing in between . The liver was processed for electron microscopy and the binding pattern of the injected markers was recorded . The electrostatic nature of the tracer binding was assessed by perfusion with high ionic strength solutions, by aldehyde quenching of the plasma membrane basic residues, and by substituting the cell surface acidic moieties with positively charged groups. Results indicate that the endothelial cells of the liver sinusoids expose on their surface both cationic and anionic residues. The density distribution of these charged groups on the cell surface is different . While the negative charge is randomly and patchily scattered all over the membrane, the cationic residues seem to be accumulated in coated pits. The charged groups co-exist in the same coated pit and bind the opposite charged macromolecule . It appears that the fixed positive and negative charges of the coated pit glycocalyx are mainly segregated in space . The layer of basic residues is located at 20-30-nm distance of the membrane, while most of the negative charges lie close to the external leaflet of the plasmalemma .

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Section: Discussionmentioning

confidence: 59%

Surface charge distribution on the endothelial cell of liver sinusoids.

Ghitescu¹,

Fixman²

1984

The Journal of Cell Biology

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“…Colloidal particles of Fe-PVS prepared by the method described had strong negative electric charges, 0.6 mA/cm for 30 min, when electrophoresed on cellulose acetate membranes (22,23). Particles gave a positive Prussian blue reaction.…”

Section: Resultsmentioning

confidence: 98%

A morphological study of ferritin synthesis in macrophages with ingested ferric hydroxide-potassium polyvinyl sulfate complexes.

Ono

Tsujii

Senol

1983

Cell Struct. Funct.

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ABSTRACT. A ferric hydroxide-polyvinyl sulfate colloidal solution (Fe-PVS), prepared by mixing potassium polyvinyl sulfate (PVSK) and ferric hydroxide colloidal solution was used to study ferritin synthesis in rat peritoneal macrophages. The colloidal particles had spherical electron opaque ferric hydroxide cores with diameters of about 250 nm surrounded by radially arranged fibrous PVS molecules. They also had strong negative electric charges.Fe-PVS particles injected into the peritoneal cavity were taken up by the macrophages then disintegrated rapidly. In the phagolysosomes the electron opaque ferric hydroxide cores of Fe-PVS were denuded of their PVS frames then decomposed into small 5-6 nm granules 24 to 48 h after injection. These small granules were released from the lysosomes into the hyaloplasm and the myelin figures were found in the lysosomal vacuoles. No reaccumulation of granules in lysosomes was found even 3 months later.The intracellular distribution of ferritin in macrophages demonstrated by the immunocytochemical method showed a pattern similar to that of the small granules formed by the disintegration of Fe-PVS. This means that in rat peritoneal macrophages that contain ingested Fe-PVS particles ferritin first is synthesized in phagolysosomes by the ferric hydroxide cores that conjugate with apoferritin or protein subunits then they are dispersed into the cytoplasm. Two possible pathways for the biosynthesis of ferritin are discussed.

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“…Seno and others have suggested that macrophages have specific sites to recognize anionic groups on foreign substances, by which macrophages bind the substances to the surface and take them up into the cytoplasm (10,11,16,23,24,26,27). The general somatic cells may not have such binding sites.…”

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confidence: 99%