Phagocytic cell chemiluminescence using different zymosan preparations

Abstract

This study compared the effectiveness of opsonized and unopsonized zymosan prepared in our laboratories with a commercially available opsonized preparation used for induction of luminol-dependent oxidative burst in phagocytic cells. The production of chemiluminescence (CL) by human whole blood, isolated human neutrophils, normal BALB c mouse splenocytes, and an immortal BALB c mouse macrophage cell line (J774A.1) was tested in an automated luminometer. Recombinant murine or human interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS) were used as priming agents in some of the experiments. With human leukocytes and normal mouse spleen cells the laboratory-prepared zymosans (regardless of opsonization) induced equal or significantly greater CL than did the commercially prepared zymosan. In addition, greatly increased CL was evident with IFN-gamma- and LPS-primed neutrophils tested with our zymosans compared with the commercial preparation. These results suggest that effective zymosans capable of inducing strong, reproducible CL responses from several different phagocytic cell populations can be readily made in the laboratory.

Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes

“…Therefore, for the demonstrated results unopsonized zymosan was used. A previous study showed that assays may be performed satisfactorily with unopsonized zymosan (14).…”

Chemiluminescent immunoassays: Discrimination between the reactivities of natural and human patient antibodies with antigens from eukaryotic pathogens, Trypanosoma cruzi and Paracoccidioides brasiliensis

“…Imaging of DNA sequences using chemiluminescence shortened the X-ray exposure time from 40 hr using a radioactive method (32P-oligomers) to <30 min (4). In clinical settings chemiluminescent methods are useful to diagnose phagocytic cell disorders, to determine the serum opsonic activity and to detect the presence of immunocomplexes in sera (5)(6)(7). All techniques of separation in solid phase generally used in the radioimmunoassays (RIAs) can also be used in chemiluminescent reactions (8).…”