Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes

Wittler, Robert R.; Lieberman, Michael M.; Paine, Donald D.; S, Muehlbauer; Lima, J.E.; Sachanandani, D M; Pinney, C A

doi:10.1128/cdli.3.5.527-532.1996

Cited by 3 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescence intensity of 1 × 10 6 cells was analyzed on a logarithmic scale, and mean fluorescence intensity was calculated with FACScan research software (BD Biosciences, San Diego, California). Production of ROS by activated neutrophils was quantitated by flow cytometric detection of dihydrorhodamine 123 oxidation to rhodamine following a previously reported procedure 32. The technique was modified by the addition of antirat CD11b‐PE and CD45‐FITC gating parameters.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the initial wave of NF‐κB activity in rat muscle reduces ischemia/reperfusion injury

et al. 2001

View full text Add to dashboard Cite

Nuclear factor kappaB (NF-kappaB) is thought to play an important role in the expression of genes expressed in response to ischemia/reperfusion (I/R) injury. In this report, the activation of NF-kappaB in rat skeletal muscle during reperfusion following a 4-h ischemic period was studied. NF-kappaB activation displayed a biphasic pattern, showing peak activities from 30 min to 3 h postperfusion and 6 h to 16 h postperfusion, with a decline to baseline binding activity levels between 3 h and 6 h. Inhibition of NF-kappaB activation was investigated using proline dithiocarbamate (Pro-DTC). NF-kappaB binding activity during reperfusion was significantly reduced by intravenous administration of Pro-DTC. Additionally, Pro-DTC resulted in decreased muscle edema and neutrophil activity, with an increased percentage of muscle survival compared with vehicle controls. These results demonstrate that NF-kappaB is activated during reperfusion in a biphasic manner and that the regulation of the initial phase of NF-kappaB activation affords physiological protection against a severe ischemic stress. Selective inhibition of NF-kappaB during early reperfusion may therefore be a therapeutic intervention for I/R injury.

show abstract

Section: Methodsmentioning

confidence: 99%

Inhibition of the initial wave of NF‐κB activity in rat muscle reduces ischemia/reperfusion injury

et al. 2001

View full text Add to dashboard Cite

show abstract

Inteferon gamma and granulocyte–macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes againstScedosporiumspp.: comparison withAspergillusspp.

et al. 2005

View full text Add to dashboard Cite

While Aspergillus spp. have been the most frequent filamentous fungi causing infections in immunocompromised patients, Scedosporium spp. are emerging as life-threatening pathogens. We studied the effects of interferon gamma (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GM-CSF) alone or combined on the antifungal activities of human polymorphonuclear leukocytes (PMN) against Scedosporium apiospermum and Scedosporium prolificans. We paralleled these activities to those against Aspergillus fumigatus and Aspergillus flavus. Incubation of PMN with IFN-gamma and GM-CSF for 22 h enhanced PMN-induced hyphal damage of both Aspergillus spp. and S. prolificans (p < 0.05) but not of S. apiospermum. However, hyphae of S. apiospermum were damaged significantly more after incubation with PMN that had been treated with IFN-gamma and GM-CSF for 2 h. In addition, incubation of PMN with GM-CSF for 2 h enhanced PMN oxidative burst measured as superoxide anion (O2-) production in response to nonopsonized hyphae of A. flavus and Scedosporium spp. (p < 0.05). In contrast, after 2 h, IFN-gamma and GM-CSF alone did not enhance PMN O2- in response to opsonized hyphae of A. flavus and Scedosporium spp.; however, the combination of IFN-gamma and GM-CSF showed significant enhancement against these species. Thus, IFN-gamma and GM-CSF, particularly in combination, demonstrate a species- and time-dependent augmentation of PMN responses to Scedosporium spp.

show abstract

Age-Related Differences of Neutrophil Activation in a Skeletal Muscle Ischemia–Reperfusion Model

Mowlavi

Reynolds²,

Neumeister³

et al. 2003

Annals of Plastic Surgery

View full text Add to dashboard Cite

Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes

Cited by 3 publications

References 31 publications

Inhibition of the initial wave of NF‐κB activity in rat muscle reduces ischemia/reperfusion injury

Inhibition of the initial wave of NF‐κB activity in rat muscle reduces ischemia/reperfusion injury

Inteferon gamma and granulocyte–macrophage colony-stimulating factor augment the antifungal activity of human polymorphonuclear leukocytes againstScedosporiumspp.: comparison withAspergillusspp.

Age-Related Differences of Neutrophil Activation in a Skeletal Muscle Ischemia–Reperfusion Model

Contact Info

Product

Resources

About