Phage typing combined with pulsed-field gel electrophoresis and random amplified polymorphic DNA increases discrimination in the epidemiological analysis of Salmonella enteritidis strains

Laconcha, Idoia; López-Molina, Nuria; Rementerı́a, Aitor; Audicana, Ana; Perales, Ildefonso; Garaizar, Javier

doi:10.1016/s0168-1605(98)00007-5

Cited by 52 publications

(44 citation statements)

References 32 publications

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“…This provides a picture different from that presented in studies in which a balanced distribution of different genotypes has been found, as evaluated by either RAPD analysis, ribotyping, or PFGE (8, 23,24). The high degree of homogeneity among Salmonella serovar Enteritidis isolates has also been described in studies in which all strains analyzed belonged to a single PT, particularly PT 4 (21,27). In our study, we found that most human isolates belonged to PT 4.…”

Section: Fig 1 Examples Of Amplification Patterns Obtained With Primentioning

confidence: 53%

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Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002

Betancor

Schelotto²,

Martinez³

et al. 2004

J Clin Microbiol

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Molecular and phenotyping techniques were applied to study Salmonella enterica serovar Enteritidis strains both from human cases of infection and of avian origin isolated in Uruguay from 1995 to 2002. A group of 62 isolates was subjected to random amplified polymorphic DNA (RAPD) assay and analysis of antibiotic resistance patterns. Twenty-one of these strains were further characterized by phage typing and analysis of their protein expression profiles. RAPD fingerprinting with five different primers discriminated 10 different genetic profiles. Of the 62 strains tested, 48 had a single major genetic profile, whereas the other nine profiles were evenly distributed among the other strains. The genetic diversity was greater among strains of animal origin than among isolates of human origin. Comparative examination of the results obtained by RAPD analysis and phenotypic analysis and by strain source provided evidence of the reliable discriminatory power of RAPD analysis in our study. Six avian isolates with antibiotic resistance were detected: two were nalidixic acid resistant and four had a particular ␤-lactam resistance pattern. The last four isolates all had the same unusual phage type (phage type 4b); however, RAPD analysis differentiated them into two groups. Two isolates with unique RAPD profiles were recovered from distinct human cases, suggesting that the technique differentiates unrelated strains. Overall, the results show the existence of a predominant genetic type that is present in poultry and that is transmitted to humans. There are also several other genotypes, but only a few of them could be recovered from human sources, suggesting the existence of different pathogenic traits among strains circulating in the country.

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Section: Fig 1 Examples Of Amplification Patterns Obtained With Primentioning

confidence: 53%

“…It offers good power for discrimination within several Salmonella serotypes, such as Dublin (20), Panama (46), Typhimurium (4), Typhi (43), and Enteritidis (7, 21,22,24,25,28). One appeal of this method is that a large number of arbitrarily selected primers can be tested to identify those that might be suitable for a particular application.…”

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confidence: 99%

Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002

Betancor

Schelotto²,

Martinez³

et al. 2004

J Clin Microbiol

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“…In recent years, many DNA-based genotyping techniques have been utilized to delineate epidemiological relationships between various isolates [23,32]. But, the combination of several methods including phage typing, serotyping, antibiogram and genetic typing may provide a powerful discriminatory tool for the epidemiological analysis of unrelated and related Salmonella strains [14,16].…”

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confidence: 99%

Characteristics of Persistent Salmonella Enteritidis Strains in Two Integrated Broiler Chicken Operations of Korea

Kwag

Bae

Cho³

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The objectives of the study were to investigate the phenotypic and genotypic characterization of the persistent Salmonella Enteritidis (S. Enteritidis) isolates in two integrated broiler chicken operations, with attention focused mainly on the epidemiological approach. In the distribution of virulence genes, Salmonella enterotoxin (stn), invading host cell (invA), and Salmonella plasmid virulence (spvC) genes were widely distributed among the S. Enteritidis irrespective of their source of isolation, and Salmonella fimbrial (sefC) and plasmid encoded fimbrial (pef) genes were present in 28 and 20 S. Enteritidis strains, respectively. A total of 5 different XbaI-PFGE types were obtained from 31 S. Enteritidis isolates. Twenty-one types were divided on the basis their PFGE pattern, phage type and antimicrobial resistance pattern determined. There was a significant difference in phenotypic and genotypic characterization by two integrated broiler operations. Also, 8 isolates shown susceptible to all antimicrobials and 11 isolates with resistance to nalidixic acid were partly classified by XbaI PFGE pattern and by the phage type.

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“…Though of limited discrimination, phage typing has been used in combination with verotoxin typing to identify linked cases of E. coli O157:H7 infection (8). Highly clonal Salmonella serovars require additional subtyping during large-scale surveillance efforts, and phage typing was useful for this (9). However, the use of phage typing was ultimately limited because it has poor resolving ability, is expensive, and requires technical expertise (10).…”

Section: Phage Typingmentioning

confidence: 99%

Navigating Microbiological Food Safety in the Era of Whole-Genome Sequencing

et al. 2016

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SUMMARYThe epidemiological investigation of a foodborne outbreak, including identification of related cases, source attribution, and development of intervention strategies, relies heavily on the ability to subtype the etiological agent at a high enough resolution to differentiate related from nonrelated cases. Historically, several different molecular subtyping methods have been used for this purpose; however, emerging techniques, such as single nucleotide polymorphism (SNP)-based techniques, that use whole-genome sequencing (WGS) offer a resolution that was previously not possible. With WGS, unlike traditional subtyping methods that lack complete information, data can be used to elucidate phylogenetic relationships and disease-causing lineages can be tracked and monitored over time. The subtyping resolution and evolutionary context provided by WGS data allow investigators to connect related illnesses that would be missed by traditional techniques. The added advantage of data generated by WGS is that these data can also be used for secondary analyses, such as virulence gene detection, antibiotic resistance gene profiling, synteny comparisons, mobile genetic element identification, and geographic attribution. In addition, several software packages are now available to generatein silicoresults for traditional molecular subtyping methods from the whole-genome sequence, allowing for efficient comparison with historical databases. Metagenomic approaches using next-generation sequencing have also been successful in the detection of nonculturable foodborne pathogens. This review addresses state-of-the-art techniques in microbial WGS and analysis and then discusses how this technology can be used to help support food safety investigations. Retrospective outbreak investigations using WGS are presented to provide organism-specific examples of the benefits, and challenges, associated with WGS in comparison to traditional molecular subtyping techniques.

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Phage typing combined with pulsed-field gel electrophoresis and random amplified polymorphic DNA increases discrimination in the epidemiological analysis of Salmonella enteritidis strains

Cited by 52 publications

References 32 publications

Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002

Random Amplified Polymorphic DNA and Phenotyping Analysis of Salmonella enterica Serovar Enteritidis Isolates Collected from Humans and Poultry in Uruguay from 1995 to 2002

Characteristics of Persistent Salmonella Enteritidis Strains in Two Integrated Broiler Chicken Operations of Korea

Navigating Microbiological Food Safety in the Era of Whole-Genome Sequencing

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