Phage displayed mimotope peptide-based immunosensor for green and ultrasensitive detection of mycotoxin deoxynivalenol

Yan, Jiaxiang; Shi, Qiang; You, Kai-hao; Li, Yanping; He, Qinghua

doi:10.1016/j.jpba.2019.01.051

Cited by 35 publications

(11 citation statements)

References 28 publications

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“…As a result, even the detection ranges were expanded to 3000 ng mL −1 by increasing the antibody level; the limit of detections to DON can be as low as 0.3 ng mL −1 . This ultra-high sensitivity in competitive immunoassay due to mimic epitope-based unequal competition was verified in our previous studies (Yan et al, 2019;Hou et al, 2019).…”

Section: Effect Of Antibody Level On Quantitative Rangesupporting

confidence: 72%

A magnetic-separation-based homogeneous immunosensor for the detection of deoxynivalenol coupled with a nano-affinity cleaning up for LC-MS/MS confirmation

Guo

Wei

et al. 2021

Food and Agricultural Immunology

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Section: Effect Of Antibody Level On Quantitative Rangesupporting

confidence: 72%

A magnetic-separation-based homogeneous immunosensor for the detection of deoxynivalenol coupled with a nano-affinity cleaning up for LC-MS/MS confirmation

Guo

Wei

et al. 2021

Food and Agricultural Immunology

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“…Therefore, substitution with mimotope is feasible for nontoxic immunoassay of OTA. Yan et al 12 used Ph.D.-12 for Mimotopes could be obtained by immunization or biopanning. For example, monoclonal Ab2, polyclonal Ab2, and Ab2β-Nb could be obtained by immunizing mice, rabbits, and camels with mAb, respectively.…”

Section: Mimotopesmentioning

confidence: 99%

“…11 According to statistics from the Food and Agriculture Organization (FAO), nearly 25% of global food production is polluted by fungi that produce high concentrations of mycotoxins. 12 Many countries have set strict national standards to control mycotoxin levels in food and feed. It is necessary to monitor the levels of mycotoxin residues to mitigate damage to humans and other animals.…”

Section: ■ Introductionmentioning

confidence: 99%

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Huang

et al. 2021

J. Agric. Food Chem.

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Mycotoxins are toxic contaminants in foods and feeds that are naturally occurring and largely unavoidable. Determining their contents in these products is essential to protect humans from harm. Immunoassays of mycotoxins have been well-established because they are fast, sensitive, simple, and cost-effective. However, a major limitation of immunoassays is the requirement of toxic mycotoxins as competing antigens, standards, or competing tracers. Mimotopes are peptides or proteins that can specifically bind to antibodies and compete with analytes for binding sites by mimicking antigenic epitopes. They can be employed as substitutes for competing antigens, standards, or competing tracers to avoid use of mycotoxins. This review summarizes the production and functionalization of the two main kinds of mimotopes, mimic peptides and anti-idiotypic antibodies (Ab2), and their applications in rapid analysis of mycotoxins.

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“…Biosensors are good alternatives for DON determination due to their high sensitivity, specificity, and rapidity. Various optical and electrochemical biosensors have been developed for DON determination [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. Evanescent wave fluorescence biosensors, combining the advantages of evanescent wave fluorescence and biomolecules binding reactions, have been widely applied for the sensitive and rapid detection of pollutants.…”

Section: Introductionmentioning

confidence: 99%

Rapid, Sensitive On-Site Detection of Deoxynivalenol in Cereals Using Portable and Reusable Evanescent Wave Optofluidic Immunosensor

Liu

Chen

et al. 2022

IJERPH

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This paper develops an improved portable and reusable evanescent wave optofluidic immunosensor (OIP-v2) for rapid and sensitive on-site determination of deoxynivalenol (DON), one of the most frequently detected mycotoxins mainly produced by Fusarium species. Using the bifunctional reagent N,N′-Disuccinimidyl carbonate, deoxynivalenol-bovine-serum-albumin (DON-BSA) were covalently modified onto a bio-probe surface as biorecognition elements, whose robustness allowed it to perform multiple detections without significant activity loss. An indirect competitive immunoassay strategy was applied for DON detection. Under optimal conditions, the limit of detection of 0.11 μg/L and the linear dynamic detection range of 0.43 to 36.61 μg/L was obtained when the concentration of the Cy5.5-anti-DON antibody was 0.25 μg/mL. The OIP-v2 was also applied to detect DON in various cereals, and the recoveries ranged from 81% to 127%. The correlation between OIP-v2 and enzyme-linked immunosorbent assay (ELISA) through the simultaneous detection of maize-positive samples was in good agreement (R2 = 0.9891).

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Phage displayed mimotope peptide-based immunosensor for green and ultrasensitive detection of mycotoxin deoxynivalenol

Cited by 35 publications

References 28 publications

A magnetic-separation-based homogeneous immunosensor for the detection of deoxynivalenol coupled with a nano-affinity cleaning up for LC-MS/MS confirmation

A magnetic-separation-based homogeneous immunosensor for the detection of deoxynivalenol coupled with a nano-affinity cleaning up for LC-MS/MS confirmation

Mimotope-Based Immunoassays for the Rapid Analysis of Mycotoxin: A Review

Rapid, Sensitive On-Site Detection of Deoxynivalenol in Cereals Using Portable and Reusable Evanescent Wave Optofluidic Immunosensor

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