Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential

Suo, Siqingaowa; Wang, Xue; Zarlenga, Dante S.; Bu, Ri e.; Ren, Yudong; Ren, Xiaofeng

doi:10.1007/s11262-015-1208-7

Cited by 12 publications

(7 citation statements)

References 33 publications

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“…The S protein of TGEV near the N terminal portion (S1) has four antigenic epitopes [26]. Researchers have reported that the S1 gene could induce better immune responses than the full-length TGEV S gene in mice [24].…”

Section: Discussionmentioning

confidence: 99%

Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model

Cao

et al. 2019

The Journal of Veterinary Medical Science

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Transmissible gastroenteritis (TGE), caused by transmissible gastroenteritis virus (TGEV), is a highly infectious disease in pigs. Vaccination is an effective approach to prevent TGEV infection. Here, we evaluated the potential of TGEV S1 as a DNA vaccine and porcine interleukin (pIL)-12 as an adjuvant in a mouse model. A DNA vaccine was constructed with the TGEV S1 gene to induce immune response in an experimental mouse model; pIL-12 was chosen as the immunological adjuvant within this DNA vaccine. The pVAX1-(TGEV-S1) and pVAX1-(pIL-12) vectors were transfected into BHK-21 cells and expressed in vitro. Experimental mice were separately immunized with each of the recombinant plasmids and controls through the intramuscular route. The lymphocytes isolated from the blood and spleen were analyzed for proliferation, cytotoxic activities, and populations of CD4+ and CD8+ cells. The titers of TGEV S1 in an enzyme-linked immunosorbent assay (ELISA) and TGEV neutralizing antibodies and the concentrations of interferon (IFN)-γ and IL-4 were also analyzed in the serum. The plasmids pVAX1-(TGEV-S1) and pVAX1-(pIL-12) could be expressed in BHK-21 cells, and the combination of pVAX1-(TGEV-S1) and pVAX1-(pIL-12) could induce a significant increase in all markers. pIL-12 could act as an immunological adjuvant in the DNA vaccine for TGEV-S1. Furthermore, the DNA vaccine prepared using TGEV-S1 and porcine IL-12 could induce excellent humoral and cellular immune responses.

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Section: Discussionmentioning

confidence: 99%

Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model

Cao

et al. 2019

The Journal of Veterinary Medical Science

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“…The S protein of TGEV has four antigenic epitopes near the N terminal portion (S1) [25] . The S1 gene has been reported to induce more impactful immune responses than the full-length S gene of TGEV in piglets [6] .…”

Section: Discussionmentioning

confidence: 99%

Adjuvant Domuz IL-12 ile Kombine Transmissible Gastroenteritis Virüs Spike Geni Taşıyan DNA Aşı Domuzlarda Bağışıklık Yanıtı İyileştirir

Li¹,

Li²,

Chen³

et al. 2018

Kafkas Univ Vet Fak Derg

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Transmissible gastroenteritis (TGE), cause by transmissible gastroenteritis virus (TGEV), is an acute digestive and highly infectious disease in piglets. In this study, porcine interleukin-12 immunological adjuvant combined with a DNA vaccine bearing the TGEV-S1 gene was used to immunize piglets, and assessed the immune response of piglets. The results showed that CD4 + and CD8 + CD28 + T lymphocytes were significantly increased in immunized piglets compared to control groups. In addition, the observed increase of IFN-γ suggested that T helper cells tended to convert to Th1 cells during immune response. HE stain and indirect immunofluorescence assays indicated obvious differences between the immunized piglets and the controls, suggesting that immunized piglets suffered less pathological changes relative to piglets in the control groups. Moreover, the levels of anti-TGEV and neutralizing antibodies in serum also indicated an effective immune response in the immunized piglets by which the pathogen was rapidly controlled. Altogether, our results suggest that the combination of pVAX1-(pIL-12) and pVAX1-(TGEV-S1) in a vaccine is capable of producing a significantly enhanced immune response in piglets.

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“…For the phage ELISA, an anti–M13-HRP mAb (GE Healthcare Life Science, 1:5000 v/v) was used. For other ELISAs, a goat anti-human IgG HRP (BioLegend, 1:5000 v/v) was used [22] . The data were processed using Graphpad Prism 5.…”

Section: Methodsmentioning

confidence: 99%

A Single Domain–Based Anti-Her2 Antibody Has Potent Antitumor Activities

Chen

Lin

et al. 2018

Translational Oncology

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Human epidermal growth factor receptor 2 (HER2) is overexpressed in approximately 20% to 30% of breast cancers and various other types of cancers, which plays a vital role in the cancer progression. Monoclonal antibodies targeting Her2 are now used in the clinic to treat Her2 overexpression cancer patients. However, relapse or resistance is frequent with the current therapies. To generate a new treatment avenue against Her2, we immunized and selected a specific anti-Her2 single domain antibody C3 for further studies. The C3-Fc antibody drove antibody-dependent cell-mediated cytotoxicity against Her2-positive tumor cells in vitro and resulted in potent antitumor growth in vivo. These data suggest that the C3-Fc antibody may provide an alternative avenue for Her2-positive cancer therapy.

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Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential

Cited by 12 publications

References 33 publications

Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model

Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model

Adjuvant Domuz IL-12 ile Kombine Transmissible Gastroenteritis Virüs Spike Geni Taşıyan DNA Aşı Domuzlarda Bağışıklık Yanıtı İyileştirir

A Single Domain–Based Anti-Her2 Antibody Has Potent Antitumor Activities

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