pH-Wert des Milieus chronischer Wunden

Dissemond, Joachim; Witthoff, M.; Brauns, T.; Haberer, Dorothea; Goos, M.

doi:10.1007/s00105-003-0554-x

Cited by 119 publications

(55 citation statements)

References 20 publications

(16 reference statements)

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“…Hence, establishing a low physiological pH might aid wound healing [27]. Chemical acidification of the wound bed is thought to reduce bioburden as the growth of most human pathogens is inhibited at pH levels <6.0 [17]. This could be confirmed by tests using MLN where acidic pH led to a significant decrease of bacterial growth.…”

Section: Discussionsupporting

confidence: 51%

“…The pH milieu in wounds has a direct as well as an indirect influence on many factors during wound healing. Studies have shown that the pH of chronic wounds most commonly has a range of 6.5-8.5 while the pH of acute, healing wounds is significantly lower [16,17]. This alkalization or ‘alkaline shift' is thought to be due to tissue necrosis and the presence of microorganisms.…”

Section: Discussionmentioning

confidence: 99%

“…However, only limited knowledge exists on the pH dependency of the antibacterial efficacy of these substances. It is of interest to investigate the influence of the pH on the performance of antiseptics or antimicrobial wound dressings as it has been shown that the pH in chronic wounds most commonly has a range of 6.5-8.5 [16,17]. This shift towards higher pH values in chronic wounds compared to acute wounds is called ‘alkaline shift'.…”

Section: Introductionmentioning

confidence: 99%

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pH Influence on Antibacterial Efficacy of Common Antiseptic Substances

Wiegand

Abel

Ruth

et al. 2015

Skin Pharmacol Physiol

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Background: Wound infection plays an important role in compromised wound healing. A high bioburden impairs healing and leads to formation of a chronic wound. Distinctly higher pH values were observed in chronic wounds compared to acute wounds. However, there is only limited knowledge of pH dependency on the antibacterial efficacy of common antimicrobial substances. Methods: This study investigated the pH influence on the antimicrobial efficacy of povidone (PVP)-iodine, silver nitrate, chlorhexidine, octenidine and polihexanide against Staphylococcus aureus and Pseudomonas aeruginosa using the agar diffusion test and microplate laser nephelometry. Results: The bactericidal activity of chlorhexidine and octenidine was mainly pH-independent in a pH range of 5.0-9.0. In contrast, polihexanide showed a significant efficacy increase at a higher pH. It was also found that the influence of the pH on antiseptics differs among species of bacteria. For instance, S. aureus exhibited an increasing sensitivity against silver nitrate with rising pH whereas the effect on P. aeruginosa was found to be distinctly decreased. The antimicrobial effect of PVP-iodine was strongly diminished with rising pH. Conclusions: The shift towards higher pH values in chronic wounds compared to acute wounds makes it imperative to know whether the antimicrobial efficacy of applied antimicrobial substances is altered by different pH levels. The results suggest that application of polihexanide might be advantageous for the management of wound infections, as both S. aureus and P. aeruginosa exhibited an increased susceptibility with rising pH.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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pH Influence on Antibacterial Efficacy of Common Antiseptic Substances

Wiegand

Abel

Ruth

et al. 2015

Skin Pharmacol Physiol

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“…it may reduce the pH of the culture media from 7 to less than 4 [50]. Acidification of the environment can inhibit microbial growth efficiently [51]. The analysis of how CAP treatment affects different culture media is the focus of an ongoing study.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Impact of Cold Atmospheric Pressure Plasma on Medical Critical Yeasts and Bacteria Cultures

Wiegand

Beier

Horn

et al. 2013

Skin Pharmacol Physiol

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Objectives: Plasma medicine focuses on the application of cold atmospheric pressure plasmas (CAPs) in or on the human body. So far, plasmas have been used to sterilize implant materials or other thermally unstable medical products and have been applied for chemical surface modifications. This study investigates the antimicrobial effect of physical plasmas on microorganisms which cause skin infections, such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, depending on the plasma source and the kind of plasma excitation used. Materials: Microorganisms were plated onto MH2 agar plates. Plasma treatment was performed using the plasma sources BLASTER MEF and kinpen 09. To investigate the antimicrobial effects, the following plasma parameters have been varied: working gas, distance from nozzle to surface, electrical power, grid spacing of treatment lines, number of treatments and work piece velocity. Results: The generated plasmas had an antimicrobial effect that depended on the chosen plasma parameters, in particular on the process gas used, the plasma power and the number of treatments performed. Thus, different reactive species were observed by optical emission spectroscopy measurement in the generated plasmas. Conclusions: The study showed that CAPs exhibit profound bactericidal and fungicidal properties in vitro. However, an important factor for the antimicrobial efficacy is the composition of the ‘chemical soup' supplied by the CAP system which can be regulated by the process gases used.

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“…In the present study, we aimed to develop microparticulate system for controlled release of antimicrobial agent in the alkaline media as open wounds tend to have a neutral or alkaline pH, predominantly in the range of 6.5-8.5. 16) In the present investigation, the effect of Glycerol (X 1 ) and NaCl (X 2 ) concentration in outer aqueous phase was selected as independent variables while the entrapment efficiency (Y 1 ), particle size (Y 2 ) and % yield (Y 3 ) were chosen as the dependent variables. The levels for these two parameters were determined from the preliminary trials.…”

mentioning

confidence: 99%

Formulation Optimization of Gentamicin Loaded Eudragit RS100 Microspheres Using Factorial Design Study

Singh

Saraf

Dixit

et al. 2008

Biological & Pharmaceutical Bulletin

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Gentamicin-Eudragit RS100 microspheres were prepared by modified double emulsion method. A 3 2 full factorial experiment was designed to study the effects of the composition of outer aqueous phase in terms of amount of glycerol (viscosity effect) and sodium chloride (osmotic pressure gradient effect) on the entrapment efficiency and % yield and microsphere size. The results of analysis of variance test for responses measured indicated that the test is significant (pϾ0.05). The contribution of sodium chloride concentration was found to be higher on entrapment efficiency and % yield, whereas glycerol produced significant effect on the mean diameter of microspheres. Microspheres demonstrated spherical particles in the size range of 33.24-60.43 m mm. In vitro release profile of optimized formulation demonstrated sustained release for 24 h following Higuchi kinetics. Finally, drug bioactivity was found to remain intact after microencapsulation. Response surface graphs are presented to examine the effects of independent variables on the responses studied. Thus, by formulation design important parameters affecting formulation characteristics of gentamicin loaded Eudragit RS100 microspheres can be identified for controlled delivery with desirable characters in terms of maximum entrapment and yield.

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pH-Wert des Milieus chronischer Wunden

Cited by 119 publications

References 20 publications

pH Influence on Antibacterial Efficacy of Common Antiseptic Substances

pH Influence on Antibacterial Efficacy of Common Antiseptic Substances

Antimicrobial Impact of Cold Atmospheric Pressure Plasma on Medical Critical Yeasts and Bacteria Cultures

Formulation Optimization of Gentamicin Loaded Eudragit RS100 Microspheres Using Factorial Design Study

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