pH-Triggered transition of silk fibroin from spherical micelles to nanofibrils in water

Chen, Peng; Kim, Hyun Suk; Park, Chi Young; Kim, Hun Sik; Chin, In‐Joo; Jin, Hyoung Joon

doi:10.1007/bf03218556

Cited by 40 publications

(41 citation statements)

References 21 publications

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“…A similar trend has been observed for significantly higher concentrations of silk fibroin (between 0.25 mg mL −1 and 8 mg mL −1 ), as well as higher concentrations of potassium phosphate (from 0.5 M to 1.25 M) 47, 50. To independently confirm the stability of different silk layers, we fabricated silk films using the dip‐assisted LbL method and exposed them to the solutions of K + phosphate buffers of different strength (0.03 M, 0.1 M, or 0.5 M, pH 5.5), followed by brief washing in Nanopure water (Figure S2).…”

Section: Resultssupporting

confidence: 80%

Cell Surface Engineering with Edible Protein Nanoshells

Drachuk

Shchepelina

Harbaugh

et al. 2013

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Natural protein (silk fibroin) nanoshells are assembled on the surface of Saccharomyces cerevisiae yeast cells without compromising their viability. The nanoshells facilitate initial protection of the cells and allow them to function in encapsulated state for some time period, afterwards being completely biodegraded and consumed by the cells. In contrast to a traditional methanol treatment, the gentle ionic treatment suggested here stabilizes the shell silk fibroin structure but does not compromise the viability of the cells, as indicated by the fast response of the encapsulated cells, with an immediate activation by the inducer molecules. Extremely high viability rates (up to 97%) and preserved activity of encapsulated cells are facilitated by cytocompatibility of the natural proteins and the formation of highly porous shells in contrast to traditional polyelectrolyte-based materials. Moreover, in a high contrast to traditional synthetic shells, the silk proteins are biodegradable and can be consumed by cells at a later stage of growth, thus releasing the cells from their temporary protective capsules. These on-demand encapsulated cells can be considered a valuable platform for biocompatible and biodegradable cell encapsulation, controlled cell protection in a synthetic environment, transfer to a device environment, and cell implantation followed by biodegradation and consumption of protective protein shells.

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Section: Resultssupporting

confidence: 80%

Cell Surface Engineering with Edible Protein Nanoshells

Drachuk

Shchepelina

Harbaugh

et al. 2013

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“…Experiments made in vitro can provide several relevant insights into the process of silk protein assembly and the formation mechanism of the silk fiber. To unravel the assembly mechanism, the reconstituted/recombinant proteins were applied for fiber assembly under specific condition [158][159][160][161][162][163]. Jin et al characterized the change of the supramolecular structure of silk fibroin with a reduction of the pH, which demonstrates the self-assembly of silk fibroin as a function of pH.…”

Section: Silk Protein Assembly and Silk Fiber Formation Mechanism On mentioning

confidence: 99%

Silk Fiber — Molecular Formation Mechanism, Structure- Property Relationship and Advanced Applications

Liu¹,

Zhang²

2014

Oligomerization of Chemical and Biological Compounds

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“…In this case, the stretching of the hydrophobic blocks that are inside SF micelles during β-sheet formation would be the responsible for fibril formation 16 .…”

Section: Resultsmentioning

confidence: 99%

Factors Controlling the Deposition of Silk Fibroin Nanofibrils during Layer-by-Layer Assembly

Moraes

Crouzier²,

Rubner³

et al. 2014

Biomacromolecules

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The layer-by-layer technique has been used as a powerful method to produce multilayer thin films with tunable properties. When natural polymers are employed, complicated phenomena such as self-aggregation and fibrilogenesis can occur, making it more difficult to obtain and characterize high-quality films. The weak acid and base character of such materials provides multilayer systems that may differ from those found with synthetic polymers due to strong self-organization effects. Specifically, LbL films prepared with chitosan and silk fibroin (SF) often involve the deposition of fibroin fibrils, which can influence the assembly process, surface properties, and overall film functionality. In this case, one has the intriguing possibility of realizing multilayer thin films with aligned nanofibers. In this article, we propose a strategy to control fibroin fibril formation by adjusting the assembly partner. Aligned fibroin fibrils were formed when chitosan was used as the counterpart, whereas no fibrils were observed when poly(allylamine hydrochloride) (PAH) was used. Charge density, which is higher in PAH, apparently stabilizes SF aggregates on the nanometer scale, thereby preventing their organization into fibrils. The drying step between the deposition of each layer was also crucial for film formation, as it stabilizes the SF molecules. Preliminary cell studies with optimized multilayers indicated that cell viability of NIH-3T3 fibroblasts remained between 90 and 100% after surface seeding, showing the potential application of the films in the biomedical field, as coatings and functional surfaces.

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pH-Triggered transition of silk fibroin from spherical micelles to nanofibrils in water

Cited by 40 publications

References 21 publications

Cell Surface Engineering with Edible Protein Nanoshells

Cell Surface Engineering with Edible Protein Nanoshells

Silk Fiber — Molecular Formation Mechanism, Structure- Property Relationship and Advanced Applications

Factors Controlling the Deposition of Silk Fibroin Nanofibrils during Layer-by-Layer Assembly

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