pH-responsive nanoparticles and automated detection apparatus for dual detection of pathogenic bacteria

Yan, Chunlei; Sun, Yang; Yao, Mingru; Jin, Xiaoyu; Yang, Qingli; Wu, Wei

doi:10.1016/j.snb.2021.131117

Cited by 13 publications

(12 citation statements)

References 33 publications

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“…Moreover, the use of a 3D paper structure embedded with a chemical reaction-related reagent can make systems simple to use [ 31 , 32 ]. An automation device can be combined with sensing systems to allow the detection of multiple bacteria in a simple manner [ 22 ]. Recently, much effort has also been given to the fabrication of enzyme-like functional NPs and their application in the detection of bacterial contamination [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Unlike traditional NP aggregation-based methods, which are mostly used for signal visualization, the chemical reaction-catalyzing NP-based strategy is minimally affected by the interference of the complex components of actual samples, providing relatively correct signals [ 45 ]. This sensing system can quantitatively detect the levels of bacteria and toxins in a broad range using digital cameras, smartphones, or mobile devices to obtain the color intensity and conduct data processing [ 22 , 23 , 34 , 36 , 112 , 113 ].…”

Section: Discussionmentioning

confidence: 99%

“…Currently, the color development stage of colorimetric systems has five potential routes for signal visualization to determine the bacteria contamination level. The first route is based on external pH change-induced pH indicator reactions [ 19 , 20 , 21 , 22 , 23 ]. In this route, visual signal readouts can yield a color change in pH indicators via external pH changes.…”

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

“…A recent study reported the development of pH-responsive NPs for the detection of bacterial contamination in food ( Figure 1 C) [ 22 ]. These pH-responsive NPs were fabricated using a phenolphthalein indicator (PP) and a thymolphthalein indicator (TP) in a self-assembly manner and functionalized by immobilizing the bacterial species-specific aptamer on each NP.…”

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

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Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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Bacterial contamination is a public health concern worldwide causing enormous social and economic losses. For early diagnosis and adequate management to prevent or treat pathogen-related illnesses, extensive effort has been put into the development of pathogenic bacterial detection systems. Colorimetric sensing systems have attracted increasing attention due to their simple and single-site operation, rapid signal readout with the naked eye, ability to operate without external instruments, portability, compact design, and low cost. In this article, recent trends and advances in colorimetric systems for the detection and monitoring of bacterial contamination are reviewed. This article focuses on pathogen detection strategies and technologies based on reaction factors that affect the color change for visual readout. Reactions used in each strategy are introduced by dividing them into the following five categories: external pH change-induced pH indicator reactions, intracellular enzyme-catalyzed chromogenic reactions, enzyme-like nanoparticle (NP)-catalyzed substrate reactions, NP aggregation-based reactions, and NP accumulation-based reactions. Some recently developed colorimetric systems are introduced, and their challenges and strategies to improve the sensing performance are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Colorimetric Detection and Monitoring Strategies Of Bacteria...mentioning

confidence: 99%

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

See 2 more Smart Citations

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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“…Nucleic-acid aptamers are widely used in the field of biosensors because they can bind to a variety of target substances with high specificity and selectivity [8][9][10][11][12]. Our group has also reported several aptamerbased relative-detection methods [13]. The aptamer has the advantages of a short screening period, low cost, easy synthesis, stability and long-term preservation, and a high affinity and specificity.…”

Section: Introductionmentioning

confidence: 99%

Dual-Enzyme-Based Signal-Amplified Aptasensor for Zearalenone Detection by Using CRISPR-Cas12a and Nt.AlwI

Yao

Yang

Wang

et al. 2022

Foods

Self Cite

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Zearalenone (ZEN) is harmful to animals and human beings, so it is very important to develop a rapid and sensitive method for the detection of ZEN. In this paper, we proposed a novel ZEN-monitoring method using two aptamers as recognition elements and EnGen LbaCas12a and Nt.AlwI nicking endonuclease as signal amplifiers. When ZEN was present, it bound to the aptamer Z0 and, Z1 was released into solution. The solution was then separated and the Nt.AlwI enzyme was added in order to form a nicking-enzyme cycle, thereby producing large amounts of the ssDNA Z3 for 30 min. The Z3 formed a CRISPR-Cas12a-Z3 complex with CRISPR-Cas12a, activated the trans-cleavage ability of Cas12a, cleaved the Quenched Reporter for 20 min, and underwent fluorescence recovery. The aptasensor was able to sensitively detect ZEN in the linear range of 1–1000 pg/mL, with a detection limit as low as 0.213 pg/mL. The detection time lasted for 2 h. Additionally, this detection technology can also be used to monitor other hazards.

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A Novel Aptamer Lateral Flow Strip for the Rapid Detection of Gram-positive and Gram-negative Bacteria

Sun

Yan

Jia³

et al. 2022

J. Anal. Test.

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pH-responsive nanoparticles and automated detection apparatus for dual detection of pathogenic bacteria

Cited by 13 publications

References 33 publications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Dual-Enzyme-Based Signal-Amplified Aptasensor for Zearalenone Detection by Using CRISPR-Cas12a and Nt.AlwI

A Novel Aptamer Lateral Flow Strip for the Rapid Detection of Gram-positive and Gram-negative Bacteria

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