A Novel Aptamer Lateral Flow Strip for the Rapid Detection of Gram-positive and Gram-negative Bacteria

Sun, Fangli; Yan, Chunlei; Jia, Qiong; Wu, Wei; Cao, Yindi

doi:10.1007/s41664-022-00239-7

Cited by 9 publications

(3 citation statements)

References 28 publications

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“…Here, vancomycin recognizes gram-positive bacteria, and aptamer is specific for Gram-negative bacteria. They reported the detection limit of this lateral flow strip as 4 CFU/mL [115]. Another antibiotic assisted AuNPs research project for the detection of bacteria was performed by Song and coworkers.…”

Section: Nanomaterial-based Optical Sensingmentioning

confidence: 98%

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Recent Advances in Optical Sensing for the Detection of Microbial Contaminants

Idil,

Aslıyüce,

Perçin

et al. 2023

Micromachines

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Microbial contaminants are responsible for several infectious diseases, and they have been introduced as important potential food- and water-borne risk factors. They become a global burden due to their health and safety threats. In addition, their tendency to undergo mutations that result in antimicrobial resistance makes them difficult to treat. In this respect, rapid and reliable detection of microbial contaminants carries great significance, and this research area is explored as a rich subject within a dynamic state. Optical sensing serving as analytical devices enables simple usage, low-cost, rapid, and sensitive detection with the advantage of their miniaturization. From the point of view of microbial contaminants, on-site detection plays a crucial role, and portable, easy-applicable, and effective point-of-care (POC) devices offer high specificity and sensitivity. They serve as advanced on-site detection tools and are pioneers in next-generation sensing platforms. In this review, recent trends and advances in optical sensing to detect microbial contaminants were mainly discussed. The most innovative and popular optical sensing approaches were highlighted, and different optical sensing methodologies were explained by emphasizing their advantages and limitations. Consequently, the challenges and future perspectives were considered.

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Section: Nanomaterial-based Optical Sensingmentioning

confidence: 98%

“…Here, vancomycin recognizes gram-positive bacteria, and aptamer is specific for Gram-negative bacteria. They reported the detection limit of this lateral flow strip as 4 CFU/mL [ 115 ].…”

Section: Materials Used For Optical Sensing Platformsmentioning

confidence: 99%

Recent Advances in Optical Sensing for the Detection of Microbial Contaminants

Idil,

Aslıyüce,

Perçin

et al. 2023

Micromachines

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“…The antibacterial activity of TO2C, T2OE, and TO6C was estimated by choosing Gram-positive Staphylococcus aureus (S. aureus), one of main pathogens in hospital and community infections [43][44][45][46] , as the representative. A dose-dependent activity of three amphiphiles against S. aureus was tested by the plate colony-counting method.…”

Section: Antibacterial Action Of Aggregatesmentioning

confidence: 99%

Self‐reporting fluorescence deciphers the antibacterial nature of cationic amphiphiles: Monomer or aggregate?

et al. 2023

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Cationic amphiphile aggregates exhibit superior antibacterial activity than monomers. However, the antimicrobial mechanism of aggregates has not been well understood because it is difficult to distinguish and monitor aggregate and monomer in antimicrobial process. Herein, three bola‐type cationic amphiphiles with aggregation‐induced emission property have been developed to show distinguishable fluorescence in their monomer and aggregate. The hydrophilicity of monomer and the stability of aggregate are finely tuned by tailoring the linkers between two quaternary ammonium end groups and tetraphenylethylene skeleton. The sensitive fluorescence switching of monomer and aggregate achieves the quantitative monitoring of dynamic interaction of three amphiphiles with bacteria. The aggregates with cationic charges first attach to bacterial surface, and the monomers subsequently dissociate from aggregates to penetrate bacterial membrane. Further, our results reveal the vital role of stability of aggregates during antimicrobial process, shedding light on the rational design of high‐efficient antimicrobials.

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