pH-responsive glycol chitosan-cross-linked carboxymethyl-&amp;beta;-cyclodextrin nanoparticles for controlled release of anticancer drugs

Wang, Yiwen; Qin, Fei; Tan, Haina; Zhang, Yan; Jiang, Miao; Lu, Mengmeng; Yao, Xin

doi:10.2147/ijn.s91906

Cited by 11 publications

(4 citation statements)

References 38 publications

(47 reference statements)

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“…Thus CMC can serve as a nanomaterial to construct nano-drug. The combination of natural medicine or chemotherapy drugs and CMC has the advantages of high-efficacy, slow-release and low side effects 8. We constructed the C-PC/CMC nanoparticles using the ionic crosslinking method, in which carboxymethyl chitosan acted as a carrier to package C-phycocyanin.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Anti-Cancer Activity of the Nano-Drug C-PC/CMC-CD59sp NPs in Cervical Cancer

Jiang¹,

Wang²,

Zhu³

et al. 2019

J. Cancer

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The novel tumor targeted nano-drug C-PC/CMC-CD59sp nanoparticles were constructed with carbocymethyl chitosan (CMC), C-phycocyanin (C-PC) and CD59 specific ligand peptide (CD59sp). The anti-tumor drug mechanism of the C-PC/CMC-CD59sp NPs was further explored in cervical cancer cells (HeLa and SiHa) in vitro and in vivo. We found that the C-PC/CMC-CD59sp NPs could inhibit the proliferation and induce G0/G1 cell cycle arrest in cervical cancer HeLa and SiHa cells, and the cell proliferation was reduced in a dose-dependent manner. We further found that the C-PC/CMC-CD59sp NPs regulated the cell cycle via up-regulating the expression of p21, and then down-regulating the expressions of Cyclin D1 and CDK4 in vivo. Compared with C-PC and C-PC/CMC NPs, the pro-apoptosis effects of the C-PC/CMC-CD59sp NPs were more significant for HeLa and SiHa cells in vitro. Moreover, the C-PC/CMC-CD59sp NPs up-regulated the expression of cleaved caspase-3 and down-regulated the expression of bcl-2. In addition, compared with C-PC and C-PC/CMC, the C-PC/CMC-CD59sp NPs significantly inhibited MMP-2 protein expression in vivo. Our data suggested that the anti-tumor effects of C-PC/CMC-CD59sp NPs were better than C-PC and C-PC/CMC NPs. Our laboratory constructed a new drug delivery system and proved the effective antitumor effects of C-PC/CMC-CD59sp, which would widen the application of C-PC as a potential anti cervical cancer drug.

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Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of Anti-Cancer Activity of the Nano-Drug C-PC/CMC-CD59sp NPs in Cervical Cancer

Jiang¹,

Wang²,

Zhu³

et al. 2019

J. Cancer

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“…Carboxymethyl chitosan (CMC) is constructed by carboxylation modification of chitosan, and because of its good water solubility and biocompatibility, non-toxic, antibacterial characteristics, CMC has been applied in many industries and researches, especially in drug carrier. Previous research found that many natural medicine or chemotherapy drugs were packaged in CMC to realize slow-release, improve efficacy, reduce less side effects and inhibit tumor growth 8 . We chose carboxymethyl chitosan as a carrier to embed C-phycocyanin by using the ionic crosslinking method (CaCl 2 acted as crosslinking agent) to prepare C-PC/CMC, which not only improved the stability of the C-PC, but also realized the slow release of C-PC.…”

Section: Introductionmentioning

confidence: 99%

The Targeted Antitumor Effects of C- PC/CMC-CD59sp Nanoparticles on HeLa Cells in Vitro and in Vivo

Wang¹,

Jiang²,

Yin³

et al. 2017

J. Cancer

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The novel C-PC/CMC-CD59sp-NPs were made by carbocymethyl chitosan (CMC) loading C-phycocyanin (C-PC) with the lead of CD59 specific ligand peptide (CD59sp) for targeting, and the characteristics and targeted anti-tumor mechanism were explored in order to realize the targeted therapy of C-PC on the growth of HeLa cells both in vitro and vivo. The targeting nanoparticles were synthesized by ionic-gelation method, and the optimal condition was selected out by orthogonal analysis. The properties of nanoparticles were observed by laser particle analyzer and dynamic light scattering (DLS) and Fourier Transform Infrared Spectrometer (FTIR). The effects of nanoparticles on the proliferation of HeLa cells in vitro were assessed by MTT assay. The mice model with tumor was constructed by subcutaneous injection of HeLa cells into the left axilla of NU/NU mice. The weight of tumor and the spleen were tested. The expression quantities of cleaved caspase-3, Bcl-2 were determined by western blot and immunofluorescent staining. Results showed the morphology of the finally prepared nanoparticles was well distributed with a diameter distribution of 200±11.3 nm and zeta potential of -19.5±4.12mV. Under the guidance of CD59sp, the targeting nanoparticles could targetedly and efficiently arrive at the surface of HeLa cells, and had obvious inhibitory effect on HeLa cells proliferation both in vitro and vivo. Moreover, the nanoparticles could induce cell apoptosis by up-regulation of cleaved caspase-3 proteins expression, but down-regulation of Bcl-2 and cyclinD1 proteins. Our study provided a new idea for the research and development of marine drugs, and supplied a theoretical support for the target therapy of anticancer drug.

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“…We also found that these CPX loaded nanoparticles drug release is pH dependent. It can release drug faster at a higher pH than around lower pH (pH 7.4 > pH 5.2) [25]. The faster drug elution at neutral pH might be due to the swelling of the polymer matrix, which is brought about by deprotonation of amine group of chitosan.…”

Section: Discussionmentioning

confidence: 99%

Bioengineered ciprofloxacin loaded chitosan nanoparticles for the treatment of Bovine Mastitis

Yadav

Gaur

Yadav

et al. 2020

Preprint

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Mastitis is the most devastating economic disease of dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or in early lactation. E. coli. and S. aureus is the main causative agent of mastitis. S. aureus has an ability to form encapsulated focuses in the udder and develop subclinical form of mastitis. This property of bacteria hinders the effective cure during lactation period. Antibiotics used for treatments have a short half-life span at the site of action because of frequent milking, therefore unable to maintain desired drug concentration for effective clearance of bacteria. We demonstrated the potential of ciprofloxacin encapsulated nanocarriers which can improve availability of drugs and could provide effective means of the treatment of mastitis. These drug loaded nanoparticles show low toxicity & slow clearance from the site of action. Antimicrobial studied against clinical strain of E.coli. and Staphylococcus aureus zone of inhibition depend on dose 0.5 mg to 2 mg/ml nanoparticles solution from 11.6 to 14.5 mm and 15 to 18mm. These nanoparticles show good antimicrobial activity in broth culture as well against bacteria.

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pH-responsive glycol chitosan-cross-linked carboxymethyl-β-cyclodextrin nanoparticles for controlled release of anticancer drugs

Cited by 11 publications

References 38 publications

Molecular Mechanism of Anti-Cancer Activity of the Nano-Drug C-PC/CMC-CD59sp NPs in Cervical Cancer

Molecular Mechanism of Anti-Cancer Activity of the Nano-Drug C-PC/CMC-CD59sp NPs in Cervical Cancer

The Targeted Antitumor Effects of C- PC/CMC-CD59sp Nanoparticles on HeLa Cells in Vitro and in Vivo

Bioengineered ciprofloxacin loaded chitosan nanoparticles for the treatment of Bovine Mastitis

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