pH-dependent fusion of phosphatidylcholine small vesicles. Induction by a synthetic amphipathic peptide.

Parente, Roberta A.; Nir, Shlomo; Szoka, Francis C.

doi:10.1016/s0021-9258(18)68843-x

Cited by 165 publications

(59 citation statements)

References 40 publications

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“…Parente et al showed that GALA induces fusion between SUVs composed of unsaturated phospholipids. 47 Upon the addition of the peptide to an SUV solution with pH 5.0, fusion was observed as measured with a lipid mixing assay and dynamic light scattering studies. In contrast, at pH 7.5 no activity was observed.…”

Section: Peptide-induced Fusionmentioning

confidence: 99%

Model systems for membrane fusion

2011

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Membrane fusion has an overarching influence on living organisms. The fusion of sperm and egg membranes initiates the life of a sexually reproducing organism. Intracellular membrane fusion facilitates molecular trafficking within every cell of the organism during its entire lifetime, and virus-cell membrane fusion may signal the end of the organism's life. Considering its importance, surprisingly little is known about the molecular-level mechanism of membrane fusion. Due to the complexity of a living cell, observations often leave room for ambiguity in interpretation. Therefore artificial model systems composed of only a few components are being used to further our understanding of controlled fusion processes. In this critical review we first give an overview of the hypothesized mechanism of membrane fusion and the techniques that are used to investigate it, and then present a selection of non-targeted and targeted model systems, finishing with current applications and predictions on future developments (85 references).

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Section: Peptide-induced Fusionmentioning

confidence: 99%

Model systems for membrane fusion

2011

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“…Although GALA-modied peptides with shorter peptide sequences would be synthetically easier, the shorter sequence destabilizes the secondary structure. 5,17 The peptides conjugated with hydrophobic tails, also known as peptide amphiphiles (PAs), form supramolecular assemblies, which can stabilize the secondary structure of the peptides. 18,19 However, the contribution of the self-assembling propensity combined with the secondary structure to the membrane activity has not been explored.…”

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confidence: 99%

The self-assembly and secondary structure of peptide amphiphiles determine the membrane permeation activity

et al. 2014

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“…This led to several years' worth of research into the peptides' interaction with membranes and the effect of membrane composition on this interaction. [96][97][98][99][100] They demonstrated that GALA induced the fusion of small unilamellar vesicles, specifically at low pH, with potential applications to target acidic diseased tissue. [101] Later, Yao et al [102] took this approach a step further by showing pH-dependent fusion between liposomes and cellular membranes using pHLIP® (pH Low Insertion Peptide).…”

Section: Peptide-mediated Membrane Fusion With Liposomesmentioning

confidence: 99%

Membrane Fusion Models for Bioapplications

Mazur

Chandrawati

2021

ChemNanoMat

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Membrane fusion is a highly evolved and significant physiological process involving the mixing of two initially distinct lipid bilayer membranes. It is concerned with communication between and within cells and plays a key role in various processes such as exocytosis, endocytosis, fertilization, viral infection, and carcinogenesis. Due to its significant role, artificial model systems using lipid membranes have been developed over the last few decades which study the membrane fusion mechanism on a molecular level. More recently, this field of research has shifted its attention towards the use of these developed models for a diverse range of biomolecular and biomedical applications. This review will focus on current applications which stem from these models including drug delivery, sensing, protocell proliferation, and others. Our opinions on the future advancements of this field will also be included.

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pH-dependent fusion of phosphatidylcholine small vesicles. Induction by a synthetic amphipathic peptide.

Cited by 165 publications

References 40 publications

Model systems for membrane fusion

Model systems for membrane fusion

The self-assembly and secondary structure of peptide amphiphiles determine the membrane permeation activity

Membrane Fusion Models for Bioapplications

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