“…13.2 ) (Ozen et al 2009 ) . PCR conditions can be strengthened by (Ozen et al 2009 ) . For STRs, the position of semi-nested PCR primers are shown by arrows ; two outside primers are used for the fi rst-round PCR, and one inside primer is used in combination with one of the outside primers for second-round PCR.…”
“…It has multiple primer • sets designed to amplify the mutation region of the gene and inside gene or closely linked-preferably not more than 2 cM distant from both the 5´ and 3´ sides of the gene to keep the possibility of a recombination event at a very low level-short tandem repeat/single nucleotide polymorphism (STR/SNP) genetic markers ( Fig. 13.2 ) (Ozen et al 2009 ) . PCR conditions can be strengthened by (Ozen et al 2009 ) .…”
“…13.2 ) (Ozen et al 2009 ) . PCR conditions can be strengthened by (Ozen et al 2009 ) . For STRs, the position of semi-nested PCR primers are shown by arrows ; two outside primers are used for the fi rst-round PCR, and one inside primer is used in combination with one of the outside primers for second-round PCR.…”
“…It has multiple primer • sets designed to amplify the mutation region of the gene and inside gene or closely linked-preferably not more than 2 cM distant from both the 5´ and 3´ sides of the gene to keep the possibility of a recombination event at a very low level-short tandem repeat/single nucleotide polymorphism (STR/SNP) genetic markers ( Fig. 13.2 ) (Ozen et al 2009 ) . PCR conditions can be strengthened by (Ozen et al 2009 ) .…”
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