Pfropfung und Chimären, unter besonderer Berücksichtigung der entwicklungsphysiologischen Problematik

Brabec, Franz

doi:10.1007/978-3-642-50088-6_53

Cited by 4 publications

(1 citation statement)

References 191 publications

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“…; Melnyk and Meyerowitz ). In grafted plants, the exchanges of heterogeneous organic substances and hormones, particularly auxin, translocated via the symplast are crucial in the establishment of the connection (Brabec ; Carr ; Melnyk and Meyerowitz ; Melnyk et al. ).…”

Section: Introductionmentioning

confidence: 99%

Phytoplasma Transmission by Heterologous Grafting Influences Viability of the Scion and Results in Early Symptom Development in Periwinkle Rootstock

Aryan

Musetti

Riedle‐Bauer

et al. 2016

Journal of Phytopathology

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The stolbur phytoplasma ‘Candidatus Phytoplasma solani’ is responsible for the grapevine disease ‘bois noir’ affecting a number of wine-growing areas in Europe. Transmission of stolbur phytoplasma to different laboratory hosts can be difficult due to the requirement of transmitting insect vectors or parasite plants. Here, heterologous grafting was used as an alternative technique for transmission of common and strongly symptomatic stolbur genotypes CPsM4_At1 and CPsM4_At6 of ‘Ca. P. solani’ to experimental host plants such as Catharanthus roseus and tomato making phytoplasma strains more accessible for molecular and experimental investigations in different plant species. Transmission was confirmed by quantitative PCR, microscopy and nested PCR followed by marker gene sequencing. In our study, the transmission of different genotypes of ‘Ca. P. solani’ resulted in distinguishable symptom development in the laboratory host C. roseus. Symptom development in grafted rootstock was observed three to 7 weeks after heterologous grafting. Survival of the graft unit was influenced by the presence of ‘Ca. P. solani’ in the scions and was clearly reduced in phytoplasma free scion – rootstock combinations

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