Recent work has demonstrated that minerals in plants are circulated between root and shoot. This occurs during the whole life time and renders possible response to changing environmental conditions. This mineral circulation occurs through intensive solute exchange between xylem and phloem in roots, stems, and leaves. The transport form of heavy metals such as iron, manganes, zinc and copper in the phloem, whether ionic or chelated, is unclear in most cases.
The unusual amino acid nicotianamine (NA) is ubiquitous throughout the plant kingdom. It is a chelator of several divalent transition metals. Its physiological role was investigated with the tomato mutant chloronerva, the only known NA‐free multicellular plant. The mutant also exhibits disturbances of its iron metabolism and that of other heavy metals. This leads, among others, to a typical intercostal chlorosis and progressive iron accumulation in the leaves. From the heavy metal chelating properties of NA and from the phenotype of the mutant chloronerva it is concluded that NA is needed for normal distribution of heavy metals in young growing tissues fed via the phloem. This function could be fulfilled by mediating phloem loading or unloading of heavy metals as well as by preventing their precipitation in the alkaline phloem sap. An attempt is made to explain the chloronerva phenotype in the light of the phloem transport hypothesis of chelated iron.
Previous physiological studies investigating the transfer of low-frequency sound into the cochlea have been invasive. Predictions about the human cochlea are based on anatomical similarities with animal cochleae but no direct comparison has been possible. This paper presents a noninvasive method of observing low frequency cochlear vibration using distortion product otoacoustic emissions (DPOAE) modulated by low-frequency tones. For various frequencies (15-480 Hz), the level was adjusted to maintain an equal DPOAE-modulation depth, interpreted as a constant basilar membrane displacement amplitude. The resulting modulator level curves from four human ears match equal-loudness contours (ISO226:2003) except for an irregularity consisting of a notch and a peak at 45 Hz and 60 Hz, respectively, suggesting a cochlear resonance. This resonator interacts with the middle ear stiffness. The irregularity separates two regions of the middle ear transfer function in humans: A slope of 12 dB/octave below the irregularity suggests mass-controlled impedance resulting from perilymph movement through the helicotrema; a 6-dB/octave slope above the irregularity suggests resistive cochlear impedance and the existence of a traveling wave. The results from four guinea pig ears showed a 6-dB/octave slope on either side of an irregularity around 120 Hz, and agree with published data.
The non-proteinogenic amino acid nicotianamine (NA) is ubiquitous among plants. In meristematic tissues it reaches concentrations of about 400/lmol (g fresh weight)-i. NA forms complexes, among others, with the metal micronutrients (MN) copper, zinc, iron and manganese (log KMeNA 18.6-8.8). Calculations of the dissociation curves of the metal-NA complexes based on the complex formation constants and on the acid dissociation constants of NA revealed their stability at the neutral or weak alkaline pH of cytoplasm and sieve tube sap. For the Mn-NA complex, dissociation begins at about pH 6.5, for all others dissociation occurs at more acid pHs. Thus, metal-NA complexes could theoretically persist also in the apoplasm and in xylem sap. The octanol water partition coefficient of NA is about 1 and those of its metal complexes arc in the range of 0.3-0.4. The reason for this shift is perhaps the negative charge of the complexes. The higher lipophilicity of the free NA indicates that the NA supply to sites of requirement is faster than the removal of the complexes as long as membranes are an integral part of the transport paths. Changing phloem transport rates of MN-NA complexes by manipulation of the cotyledon apoplasm of Ricinus communis L. suggest a competition of MN for NA at the site(s) of phloem loading. Thus, NA could control MN transport via phloem including recirculation.
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