A b s t r a c t. The effect of the herbicide acetochlor on root growth was studied by a non-destructive electrical impedance and capacitance method in pot experiments on maize. Acetochlor was applied both as single active ingredient and mixed with safener AD-67 in two dosages. Without safener addition, acetochlor had a permanent inhibiting effect on plant root expansion. The safener AD-67 was capable of providing protective effect against herbicide application. High correlations between root electrical impedance or capacitance and the root dry mass or surface area under our laboratory conditions were confirmed by plant harvest method. Root electrical impedance and capacitance measurements proved to be valid for monitoring the effect of the herbicide influencing root development and for distinguishing plant groups subjected to different stress conditions. K e y w o r d s: acetochlor, electrical capacitance, electrical impedance, maize, root growth
INTRODUCTIONWater and nutrient uptake is a key function of plant roots. Conventional root investigation methods ie soil cores, monoliths, in-growth cores are inefficient and inherently destructive, thus unadapted for continuous monitoring of root growth. Non-destructive techniques, such as minirhizotron, isotopes, magnetic resonance imaging (MRI), X-ray imaging or ground penetrating radar also have their own limitations (Cao et al., 2010).Electrical impedance (EI) and capacitance (EC) measurements are capable to provide an assessment about in situ root status without plant damaging. The EC method was first developed by Chloupek (1972) using several crop plants under greenhouse and field conditions. Root EI and EC proved to be directly correlated with various root properties, such as fresh or dry weight, root surface area and root volume. Dalton (1995) presented a conceptual model in which root surface was considered to be the surface area of a group of cylindrical condensers. Since electrical attributes are sensitive to edaphic factors, the effect of soil type, soil water saturation and ionic status, as well as plant electrode placement can be considered at using electrical measurements (Dalton, 1995;Ozier-Lafontaine and Bajazet, 2005).The EI and EC method have been used to demonstrate the response of plants to different abiotic and biotic stress factors: cold acclimation, freeze-thaw injury, drought stress, nutrient deficiency or pathogen infection can be detected by electrical measurement (Greenham et al., 1982;Higgins et al., 1977;Räisänen et al., 2007;Repo et al., 1994). Since root EI and EC depend on root mass or on active root surface, the method appears to be valid for monitoring the effect of chemicals exerting influence on root expansion. Acetochlor is a herbicide active ingredient of the chloroacetanilide family, used as a pre-emergent and early post-emergent agent for the control of annual grasses and dicotyledonous weeds on maize fields. It acts as a growth inhibitor by suppressing the anabolic pathways of fatty acids, proteins and gibberellic acids (Abu-Qar...