The root electrical capacitance (CR) method is suitable for assessing root growth and activity, but soil water content (SWC) strongly influences the measurement results. This study aimed to adapt the method for field monitoring by evaluating the effect of SWC on root capacitance to ensure the comparability of CR detected at different SWC. First a pot experiment was conducted with maize and soybean to establish CR–SWC functions for the field soil. Ontogenetic changes in root activity were monitored under field conditions by simultaneously measuring CR and SWC around the roots. The CR values were normalized using SWC data and experimental CR–SWC functions to obtain CR*, the comparable indicator of root activity. The effect of arbuscular mycorrhizal fungi (AMF) inoculation on the CR* and biomass of field-grown soybean was investigated. The pot trial showed an exponential increase in CR with SWC. CR–SWC functions proved to be species-specific. CR showed strong correlation with root dry mass (R2 = 0.83–0.87). The root activity (CR*) of field-grown crops increased until flowering, then decreased during maturity. This was consistent with data obtained with other methods. AMF inoculation of soybean resulted in significantly higher CR* during the late vegetative and early flowering stages, when destructive sampling concurrently showed higher shoot biomass. The results demonstrated that the root capacitance method could be useful for time course studies on root activity under field conditions, and for comparing single-time capacitance data collected in areas with heterogeneous soil water status.
Appropriate selection and well-timed measurement of plant developmental, morphological and physiological parameters are essential to maximize efficacy and minimize time consumption of experiments. To select for the most sensitive indicators of drought or salt stress, three independent pot experiments with diverse setups were analysed with 20-20 measured parameters. Parameters of plant growth, phenology and symbiotic interactions, visual stress symptoms, photosynthetic activity, nutrient composition and vitality were studied and the result matrices were evaluated with principal component analysis (PCA). Stress effects manifested in PC1 of two experiments and in PC2 of the third one. Traits assumed to be adequate for stress indication were characterized by high PC1 or PC2 loading values. Beside parameters of biomass production, growth and visible stress symptoms, less evident traits e.g. root electrical capacitance, membrane stability index in roots and leaves, relative water content of leaves and SPAD units were identified.
A b s t r a c t. Importance of phase angle measurement during the application of electrical impedance spectroscopy was studied by executing pot experiments with maize. Electrical impedance, phase angle (strength of capacitive character), and dissipation factor in the plant-soil system were scanned between 100 and 10 000 Hz current frequency. The frequency-dependent change in the phase angle could be described by optimum curves culminating within 920-3 650 Hz. Since the rate of energy dissipation is independent of root extent, the higher phase angle and lower energy dissipation were associated with the higher coefficient of determination achieved for the root electrical impedance -root system size (root dry mass and root surface area) regressions. The characteristic frequency selected on the basis of phase angle spectra provided a higher significance level at statistical comparison of plant groups subjected to stress conditions influencing root development. Due to the physicochemical changes observable in aging root tissue, the apex of phase angle spectra, thus the characteristic frequency, shifted continuously toward the higher frequencies over time. Consequently, the regularly repeated phase angle measurement is advisable in time-course studies for effective application of the electrical impedance method, and the systematic operation at the same frequency without determination of phase angle spectra should be avoided.K e y w o r d s: dissipation factor, root capacitance, electrical impedance spectroscopy, phase angle, root surface area INTRODUCTIONDestructive root investigation methods, such as soil cores, in-growth cores, or monoliths are unsuitable for continuous monitoring of root development or activity in response to changing environmental conditions. The applicability of the non-destructive ground penetrating radar, radioactive tracers, MRI or X-ray imaging techniques is also strongly limited in many cases: providing little resolution of root structure (detect clearly coarse roots only), these methods are not adapted for quantification of root surface area and examination of many plant phenomena related to root development (Cao et al., 2010;Èermák et al., 2006). Conversely, electrical impedance (EI) and electrical capacitance (EC) measurements in a plant-soil system offer good opportunities of rapid in situ investigation of the root system size and root activity without any intrusion into plant life function. By fixing an electrode at the plant stem and embedding the other one in the soil and connecting them by an LCR-instrument, the measured root EI and EC are directly correlated with root mass, root length, or root surface area (Chloupek, 1972;Ozier-Lafontaine and Bajazet, 2005;Rajkai et al., 2002). EI and EC methods have been used for investigation of detached plant tissues and organs subjected to various stress conditions (cold acclimation, freeze-thaw injury, drought, nutrient deficiency, or pathogen infection) as well as for studying intact root systems of plants cultivated in soil or grown in hydro...
16Background and aims The measurement of electrical capacitance in root-soil system (C R ) is a useful method for
Soybean (Glycine max L. Merr.) is regarded worldwide as indisputably one of the most important crops for human food and animal feed. The presence of symbiotic bacteria and fungi is essential for soybean breeding, especially in low-input agricultural systems. Research on the cooperation between different microbial symbionts is a key to understanding how the health and productivity of the plant is supported. The symbiotic effectivity of dual and tripartite symbiotic agents was investigated in two pot experiments on different soybean cultivars with special regard to compatibility. In the Selection experiment, two out of sixteen soybean cultivars (Aliz, Emese) were chosen on the basis of their drought tolerance and used in all the other investigations. In the Compatibility experiment, the compatible coupling of symbiotic partners was selected based on the efficiency of single and co-inoculation with two Bradyrhizobium japonicum strains and two commercial arbuscular mycorrhizal fungal (AMF) products. Significant differences were found in the infectivity and effectivity of the microsymbionts. The rhizobial and AMF inoculation generally improved plant production, photosynthetic efficiency and root activity, but this effect depended on the type of symbiotic assotiation. Despite the low infectivity of AMF, inocula containing fungi were more beneficial than those containing only rhizobia. In the Drought Stress (DS) experiment, co-inoculated and control plants were grown in chernozem soil originating from organic farms. Emese was more resistant to drought stress than Aliz and produced a bigger root system. Under DS, the growth parameters of both microbially inoculated cultivars were better than that of control, proving that even drought tolerant genotypes can strengthen their endurance due to inoculation with AMF and nitrogen fixing bacteria. Root electrical capacitance (CR) showed a highly significant linear correlation with root and shoot dry mass and leaf area. The same root biomass was associated with higher CR in inoculated hosts. As CR method detects the absorptive surface increasing due to inoculation, it may be used to check the efficiency of the microbial treatment.
In Hungary, plantations of Pinus nigra Arn. (Austrian pine) involve large areas of dolomite rock grasslands and have caused the impoverishment or local extinction of the original flora. In addition to these conservation concerns, an important economic problem is the flammability of these forests. Fire risk depends on the amount of accumulated flammable organic components. Thus, the purpose of our research was to quantify the mass of litter accumulated in Austrian pine stands and to examine the correlation between litter mass, stand age, and slope aspect. Forty-eight sampling sites were selected with stand ages ranging from 21 to 108 years. Stands represented four age classes and three exposure types. At each sampling site, litter mass was determined in the following three fractions: needles, branches, and cones. The litter fractions showed their maximum quantities in age class 61–80 years (needles = 17 560 kg/ha, branches = 2764 kg/ha, and cones = 2960 kg/ha). For the needle litter, a significant increase with age was detected through the age classes of 21–40, 41–60, and 61–80 years, and then a significant decrease occurred in stands above 80 years. In the case of branch litter, the age-dependent increase was again significant to its maximum quantity, but the decrease in old stands proved to be insignificant. With cone litter, age dependence could not be detected. Exposure of the stands had no effect on the quantities of the three litter fractions. The amount of accumulated litter of Austrian pine stands many times exceed the litter quantity of the rock grasslands (the original vegetation prior to afforestation). Furthermore, it is two or three times higher than the amount of litter reported from native zonal forests of Hungary. Therefore, the Austrian pine stands are subjected to an increased risk of fire, especially in age class 61–80 years.
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