“…The Cienega region Jalisco is important at national level for its contributions to the production of wheat (68.05%), sorghum (48.05%), corn (27.75%), agave (4.8%), and red tomato (4.0%) (Transparencia 2004). It has been reported that the most frequently applied pesticides in agricultural fields in Jalisco, Mexico (Ayuquila-Armería river basin in Autlán de Navarro) are glyphosate, glufosinate, diazinon, parathion, malation, cypermethrin, lambda cialotrina, and paraquat (Pérez-Herrera et al 2018;Rodríguez-Aguilar et al 2019;Guzmán-Plazola et al 2016). In this study, results from the Cienega region are consistent with the high frequencies of application of glyphosate, glufosinate, cypermethrin, and paraquat, with a 60.49% prevalence in the use of…”