Peste des Petits Ruminants Virus in Tibet, China

Abstract

Serologic and molecular evidence indicates that peste des petits ruminants virus (PPRV) infection has emerged in goats and sheep in the Ngari region of southwestern Tibet, People’s Republic of China. Phylogenetic analysis confirms that the PPRV strain from Tibet is classified as lineage 4 and is closely related to viruses currently circulating in neighboring countries of southern Asia.

Phylogenetic analysis of peste des petits ruminants virus from outbreaks in Turkey during 2008–2012

“…PPR viruses belonging to lineages I and II have been isolated exclusively from west and central African countries including the Ivory Coast, where the PPR virus was first detected in the 1940s (Chard et al, 2008;Luka et al, 2011;Munir et al, 2012b). Lineage III has been isolated from eastern Africa and the Arabian peninsula (Shaila et al, 1996;Kwiatek et al, 2011); lineage IV has been isolated in Asia (Kwiatek et al, 2007;Kerur et al, 2008;Wang et al, 2009;Balamurugan et al, 2010;Munir et al, 2012a;Anees et al, 2013), in the Middle East (Esmaelizad et al, 2011), and more recently in northern Africa (Sanz-Alvarez et al, 2008;Kwiatek et al, 2011;De Nardi et al, 2012 locations 5737-5761 and 6184-6160, respectively (Özkul et al, 2002). The RT-PCR reaction was performed with a 1-step RT-PCR kit (QIAGEN) in a final volume of 20 µL, which contained 4 µL of 5X 1-step RT-PCR buffer, 0.4 µM of each primer, 0.8 µL of 10 mM of each dNTP, 0.8 µL enzyme mix, 4 µL of 5X Q solution, 7 µL of PCR-grade water, and 2.6 µL of sample RNA.…”

Sequence analysis of peste des petits ruminants virus from ibexes in Xinjiang, China

“…In July 2007, a PPR outbreak was reported in the Ngari region of southwestern Tibet, China (Wang et al, 2009). In December 2013, a new outbreak of PPR was reported in Xinjiang Province, and PPR was also detected in Gansu, Inner Mongolia, Ningxia, Jiangxi, and Hunan Provinces by mid-March 2014 (Wang et al, 2015).…”

Comparison of genes as target for molecular diagnosis of peste des petits ruminants in goats

“…Reverse transcriptase polymerase chain reaction (RT-PCR), a molecular diagnostic test based on amplification of the gene target offers a new strategy for diagnosis of PPRV, and is more sensitive than other tests. RT-PCR also offers the possibility of analysing the relationship between different PPRV strains for epidemiological studies (Shaila et al, 1996;Ozkul et al, 2002;Kwiatek et al, 2007;Wang et al, 2009;Balamurgan et al, 2010). Although RT-PCR overcomes the limitations of conventional tests, the sensitivity varies depending on the primer used and the gene targeted.…”