Pesquisa de oocistos de Cryptosporidium sp em fezes: comparação entre os métodos de Kinyoun modificado e de heine

Neto, Vicente Amato; Braz, Lúcia Maria Almeida; Pietro, A O Di; Modolo, José Rafael

doi:10.1590/s0037-86821996000600008

Cited by 11 publications

(3 citation statements)

References 6 publications

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“…42 Children with clinical gastrointestinal findings, but with no infections by intestinal protozoa, could be infected by other pathogens such as bacteria or viruses. [43][44][45] However, examination of only one fecal sample, as in this study, does not eliminate infections by other parasites, which require exami- nation of three consecutive samples for a more accurate diagnosis. Some years ago, amebiasis was among the main causes of diarrhea in children in Mexico City.…”

Section: Discussionmentioning

confidence: 99%

Cryptosporidiosis and Other Intestinal Protozoan Infections in Children Less Than One Year of Age in Mexico City

Vega¹,

Tay-Zavala²,

Aguilar-Chiu³

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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Parasitic diseases are very important in Mexico because of their economic impact and adverse effects on normal growth in children. Cryptosporidiosis has been associated with acute diarrhea in immune competent and incompetent human hosts, fecal contamination of drinking water sources, and handling of animals. Due to the lack of reports on cryptosporidiosis in Mexico, we conducted a parasitologic study in children with diarrhea and other clinical symptoms. The main objectives were 1) to determine the prevalence of cryptosporidiosis in children less than one year of age in Mexico City, and 2) to correlate Cryptosporidium infection with gastrointestinal symptoms. Two hundred fecal samples from children seen at the Gabriel Mancera Familiar Medicine Unit of the Instituto Mexicano del Seguro Social were studied. Children were divided into two groups. Group A was composed of sick children with 6-8 watery diarrheic episodes every 24 hours attended at the emergency service. Group B was composed of healthy babies getting routine check ups. Only children in group A were found to be infected with intestinal protozoa (50% with Giardia lamblia, 41% with Cryptosporidium spp., and 4% with Entamoeba histolytica). The results suggested a high incidence of Cyrptosporidium infections in children in Mexico City, which make these observations useful for future studies.

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Section: Discussionmentioning

confidence: 99%

Cryptosporidiosis and Other Intestinal Protozoan Infections in Children Less Than One Year of Age in Mexico City

Vega¹,

Tay-Zavala²,

Aguilar-Chiu³

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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“…Oocysts of Eimeria and Cryptosporidium as well as cysts of Entamoeba and Giardia were also detected (Table 1). In order to confirm the presence of Cryptosporidium spp., oocysts were stained using Kinyoun modified method (AMATO NETO et al, 1996). All samples positive for Cryptosporidium spp in the centrifugal flotation technique were also positive according to the Kinyoun method.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of gastrointestinal parasites in goats from the Western Santa Catarina, Brazil

Radavelli

Pazinato

Klauck

et al. 2014

Rev. Bras. Parasitol. Vet.

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This study aims to investigate the occurrence of gastrointestinal parasites in goats from the Western Santa Catarina, Brazil. Twenty four farms were analyzed in 17 different municipalities. Animals (n=217) from different production purposes (milk and meat) and age were randomly chosen. Fecal samples were collected from the rectum stored in plastic bottles and transported to the laboratory in portable coolers at 10 °C. The technique of centrifugal flotation with saturated sugar solution was carried out in order to investigate the presence of eggs, cysts, and oocysts of gastrointestinal parasites. In 88.9% of the investigated animals, it was observed that the presence of nematode eggs which belongs to the Strongylida order, after cultivation and larvae identification were identified as Haemonchus spp., Trichostrongylus spp., Teladorsagia spp., Cooperia spp., and Oesophagostomum spp. Eggs of Thysanosoma, Trichuris, Moniezia, and Neoascaris genus were also observed. Additionally, the presence of oocysts of Eimeria spp. and Cryptosporidium spp. as well as cysts of Giardia spp., and Entamoeba spp. were verified. In all the farms evaluated, the animals showed a single or mixed infection, with the highest occurrence of helminths belonging to the Haemonchus and Trichostrongylus genus, as well as the protozoan Eimeria.Keywords: Goats, endoparasites, Strongylida, Eimeria, protozoa. ResumoO presente estudo objetivou investigar a ocorrência de parasitas gastrointestinais que infectam caprinos na região Oeste de Santa Catarina, Brasil. Foram analisadas 24 propriedades localizadas em 17 municípios, totalizando 217 caprinos. Os animais de diferentes categorias de produção (leite ou carne) e idade foram escolhidos aleatoriamente. A coleta das fezes foi realizada diretamente da ampola retal dos animais, armazenadas em frascos plásticos e transportadas para o laboratório em caixas térmicas a 10 ºC. A técnica de centrífugo-flutuação com solução saturada de açúcar foi utilizada para pesquisar a presença de ovos, cistos e oocistos de parasitos. Em 88,9% dos animais analisados foi verificada a presença de ovos de nematóides da ordem Strongylida e, após cultivo, foram identificadas larvas de Haemonchus spp., Trichostrongylus spp., Teladorsagia spp., Cooperia spp. e Oesophagostomum spp. Foram identificados também ovos de parasitos dos gêneros Thysanosoma, Trichuris, Moniezia e Neoascaris. A presença de oocistos de Eimeria spp. e Cryptosporidium spp., e cistos de Giardia spp., e Entamoeba spp. foram observadas. Em todas as propriedades avaliadas, os animais apresentavam infecções simples ou mistas, sendo a maior ocorrência de helmintos do gênero Haemonchus e Trichostrongylus, assim como pelo protozoário Eimeria.

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“…The most common staining techniques for demonstrating Cryptosporidium sp oocysts in feces are Giemsa, auramine, modified Ziehl-Neelsen, dimethyl sulphoxide, safranine and Heine, as well as looking for fluorescent mononuclear antibodies (1,2,14,23,25,26). The number of slides analyzed and fecal samples collected from each patient varies depending on the author (2,16,27).…”

Section: Introductionmentioning

confidence: 99%

Cryptosporidium SP in HIV-infected individuals attending a Brazilian University Hospital

Langoni-Júnior¹,

Souza²

2007

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:The aim of the current work was to evaluate the occurrence of Cryptosporidium sp in AIDS patients in a region of São Paulo State, Brazil. Patients were divided into groups according to CD4 + T lymphocyte count and use of potent antiretroviral treatment. Two hundred and ten fecal samples from 105 patients were fixed in 10% formalin and subjected to centrifuge formol-ether sedimentation. Slides were stained with auramine and confirmed by modified Ziehl-Neelsen.Cryptosporidiosis occurrence was 10.5% with no relationship among gender, age or the presence of diarrhea. The number of oocysts in all samples was small, independent of CD4 + T lymphocyte count, HIV plasma viral load, and presence of diarrhea. These results may be due to the reduced prevalence of opportunistic infections in AIDS individuals after the advent of highly active antiretroviral therapy.

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Pesquisa de oocistos de Cryptosporidium sp em fezes: comparação entre os métodos de Kinyoun modificado e de heine

Cited by 11 publications

References 6 publications

Cryptosporidiosis and Other Intestinal Protozoan Infections in Children Less Than One Year of Age in Mexico City

Cryptosporidiosis and Other Intestinal Protozoan Infections in Children Less Than One Year of Age in Mexico City

Occurrence of gastrointestinal parasites in goats from the Western Santa Catarina, Brazil

Cryptosporidium SP in HIV-infected individuals attending a Brazilian University Hospital

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