Perturbation of lignin biosynthesis pathway in <i>Brassica napus</i> (canola) plants using RNAi

Bhinu, V.-S.; Li, Rong; Huang, Jun; Kaminskyj, Susan G. W.; Sharpe, Andrew; Hannoufa, Abdelali

doi:10.4141/cjps08164

Cited by 20 publications

(16 citation statements)

References 39 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We recently showed that it was possible to aVect lignin (polymerized monolignols) content and composition in B. napus by altering the expression of genes in the phenylpropanoid pathway (Bhinu et al 2009b). While diVerences in the composition are beyond the scope of this work, we determined the lignin content in lines with mutations in the two genes.…”

Section: Resultsmentioning

confidence: 98%

Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis

Huang

Bhinu

et al. 2009

Planta

View full text Add to dashboard Cite

Hydrocinnamic acid esters, lignin, flavonoids, glucosinolates, and salicylic acid protect plants against UV exposure, oxidative stress, diseases, and herbivores. Through the phenylpropanoid pathway, certain Brassicaceae family members, including Arabidopsis thaliana and Brassica napus, accumulate large amounts of the anti-nutritive sinapoylcholine (sinapine) in the seed. We successfully down-regulated activities of key enzymes in the pathway including F5H and SCT and achieved reduction of sinapine and lignin in B. napus seeds. Despite this success, it was unclear how multiple agronomic traits were affected in the transgenic plants. Here, we report altered large-scale gene expression of new alleles of f5h and sct mutants of A. thaliana and resultant accumulation of sinapoylglucose, disinapoylglucose, quercetin-3-O-rhamnoside, salicylic acid glucoside, and total indolyl glucosinolates in the two mutants. Expression of several flowering genes was altered in these mutants when grown under drought and NaCl treatments. Furthermore, both mutants were more susceptible to fungal infection than the wild type. Microarray experiments identified distinctive spatial and temporal expression patterns of gene clusters involved in silique/seed developmental processes and metabolite biosynthesis in these mutants. Taken together, these findings suggest that both f5h and sct mutants exhibit major differences in accumulation of diverse metabolites in the seed and profound changes in global large-scale gene expression, resulting in differential pleiotropic responses to environmental cues. Electronic supplementary material The online version of this article (doi:10.1007/s00425-009-1007-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 98%

Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis

Huang

Bhinu

et al. 2009

Planta

View full text Add to dashboard Cite

show abstract

“…RNAi targeting of COMT reduced lignin content by 6.4–14.7% in switchgrass [4, 29, 30]. Other research has reported greater reductions of lignin content of 20% in alfalfa [13], 35% in Brassica napus [62] and 40% in canola [28]. …”

Section: Discussionmentioning

confidence: 99%

“…RNAi downregulation of COMT expression in canola [28], alfalfa [12, 13], switchgrass [4, 29, 30], and sugarcane [31, 32] consistently resulted in decreases in S monomers, as well as an overall decrease in total lignin content. Similar results have been found after sense or antisense downregulation of COMT in alfalfa [14, 23, 33, 34], poplar [35], tobacco [20], and maize [36, 37].…”

Section: Introductionmentioning

confidence: 99%

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production

Bewg

Poovaiah

Lan

et al. 2016

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundSugarcane is a subtropical crop that produces large amounts of biomass annually. It is a key agricultural crop in many countries for the production of sugar and other products. Residual bagasse following sucrose extraction is currently underutilized and it has potential as a carbohydrate source for the production of biofuels. As with all lignocellulosic crops, lignin acts as a barrier to accessing the polysaccharides, and as such, is the focus of transgenic efforts. In this study, we used RNAi to individually reduce the expression of three key genes in the lignin biosynthetic pathway in sugarcane. These genes, caffeoyl-CoA O-methyltransferase (CCoAOMT), ferulate 5-hydroxylase (F5H) and caffeic acid O-methyltransferase (COMT), impact lignin content and/or composition.ResultsFor each RNAi construct, we selected three events for further analysis based on qRT-PCR results. For the CCoAOMT lines, there were no lines with a reduction in lignin content and only one line showed improved glucose release. For F5H, no lines had reduced lignin, but one line had a significant increase in glucose release. For COMT, one line had reduced lignin content, and this line and another released higher levels of glucose during enzymatic hydrolysis. Two of the lines with improved glucose release (F5H-2 and COMT-2) also had reduced S:G ratios.ConclusionsAlong with improvements in bagasse quality for the production of lignocellulosic-based fuels, there was only one line with reduction in juice sucrose extraction, and three lines with significantly improved sucrose production, providing evidence that the alteration of sugarcane for improved lignocellulosic ethanol production can be achieved without negatively impacting sugar production and perhaps even enhancing it.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0683-y) contains supplementary material, which is available to authorized users.

show abstract

“…Bhinu et al (2009) cambiaron la vía metabólica de la lignina en la semilla de Brassica napus L. (canola) y llevó a la formación de lignina con una mayor concentración de grupos aldehídos.…”

Section: Discussionunclassified

Perfil lignoídico del tegumento de semillas de Araucaria angustifolia

et al. 2016

View full text Add to dashboard Cite

SUMMARYThe aim of this research was to quantify and identify the types of lignin and characterize its functionalization based on apparent physical resistance in each seed coat layers of Araucaria angustifolia. Each seed layer (exotesta, mesotesta and endotesta) was milled, homogenized and pre-extracted with solvents: cyclohexane, ethyl acetate and methanol for quantification of lignin. Pre-treatment with NaOH 1% was also performed in each layer of the seed for lignin quantification and for infrared spectroscopy. The lignin content obtained in the testa was 32.43 % for the three layers. After alkaline treatment, the lignin content was 31.52 % in exotesta, 16.88 % in mesotesta and 15.48 % in endotesta. Dioxane lignin was isolated from the seed coat and reduced with NaHB 4 . Infrared spectra (FTIR) were obtained from the dioxane lignin, reduced dioxane lignin and alkaline-treated Klason lignin of each layer. Microscopic analyses of the in natura exotesta and mesotesta layers from the seed coat were performed through histochemical test (Wiesner) and fluorescence microscopy. Infrared analyses made possible observation of aldehyde terminations in dioxane lignin and propose the structural profile of Klason lignin in different layers. Fluorescence microscopic analyses allowed identification of highly lignified macrosclereids in exotesta.Key words: lignin, nut, infrared, testa. RESUMENEl objetivo de esta investigación fue identificar y cuantificar los tipos de lignina, y caracterizar su función según la resistencia física aparente de cada capa de la testa de la semilla de Araucaria angustifolia. Cada capa de la semilla (exotesta, mesotesta e endotesta) se molió, homogeneizó y sometió a un ciclo de pre-extracción con solventes (ciclohexano, acetato de etilo y metanol) para preparar el material para cuantificar la lignina. También se realizó en cada capa de la semilla un pre-tratamiento con NaOH 1 % para cuantificar la lignina y para aplicar espectroscopía infrarroja. El contenido de lignina en la testa fue 32,43 % para las tres capas juntas. Después del tratamiento alcalino, el contenido de lignina fue de 31,52 % en exotesta, 16,88 % en mesotesta y 15,32 % en endotesta. Lignina dioxano se aisló del tegumento de la semilla y la misma se redujo químicamente con NaHB 4 . Los espectros infrarrojos se registraron a partir de lignina dioxano, lignina dioxano reducida y de lignina de Klason de cada capa con tratamiento alcalino. Se realizaron análisis microscópicos de las capas de exotesta y mesotesta de la testa de la semilla al natural, por prueba histoquímica (prueba Wiesner) y por microscopía de fluorescencia. El análisis infrarrojo permitió observar la presencia de terminales aldehído en lignina dioxano y trazar el perfil estructural de las ligninas de Klason en capas diferentes. En el análisis microscópico con fluorescencia se identificó en la exotesta la presencia de macroesclereidas altamente lignificadas.Palabras clave: lignina, piñón, infrarrojo, testa. INTRODUCCIÓNUna gran parte de las semillas forestales en Brasil se...

show abstract

Perturbation of lignin biosynthesis pathway in Brassica napus (canola) plants using RNAi

Cited by 20 publications

References 39 publications

Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis

Pleiotropic changes in Arabidopsis f5h and sct mutants revealed by large-scale gene expression and metabolite analysis

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production

Perfil lignoídico del tegumento de semillas de Araucaria angustifolia

Contact Info

Product

Resources

About