Pertumbuhan, Produksi Biomassa, dan Kandungan Glikosida  Steviol pada Lima Klon Stevia Introduksi di Bogor, Indonesia

Sinta, Masna Maya; Sumaryono, dan

doi:10.24831/jai.v47i1.20653

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…The survival rate of stevia in the field also depends on the clones. Research conducted by Sinta & Sumaryono (2019) on stevia planted on the high altitude area showed a decrease in stevia's survival rate by 0-30% depending on stevia clones. In this research, stevia clone BS 22 was used as explant, where BS 22 was a stevia mutant plants with high content of reb A and stevioside (Sinta, 2018).…”

Section: 7 10mentioning

confidence: 99%

Acclimatization and early growth of tissue culture-derived Stevia rebaudiana at low altitude area in Bogor, Indonesia (Aklimatisasi dan pertumbuhan awal Stevia rebaudiana asal kultur jaringan pada dataran rendah di Bogor, Indonesia)

Sinta¹,

Amanah²

2019

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Aklimatisasi merupakan masa transisi sebelum kultur in vitro dapat ditanam di lingkungan ex vitro. Di daerah tropis, stevia seyogianya ditanam di dataran tinggi. Pengembangan klon stevia yang sesuai untuk dataran rendah di kawasan tropis sangat penting untuk memungkinkan penggunaan mekanisasi pada pertanaman stevia yang luas. Tujuan penelitian ini adalah menentukan pengaruh kondisi eksplan dan periode hardening terhadap daya hidup dan pertumbuhan pada tahap aklimatisasi dan pertumbuhan awal stevia klon BS 22 pada area terbuka di dataran rendah di wilayah tropis. Penelitian pertama dilangsungkan menggunakan umur tunas yang berbeda: 0, 1, 2, dan 3 minggu yang dikultur pada media padat sebagai sumber bahan eksplan. Penelitian kedua menggunakan satu buku stevia pada periode hardening dalam media cair selama 1, 4, 7 dan 10 hari. Aklimatisasi dilaksanakan dengan menanam eksplan dalam medium tumbuh campuran pada multi-tray dan diletakkan di dalam sungkup plastik tertutup selama 1 bulan. Tanaman yang berhasil hidup kemudian dipindah ke polibeg pada area terbuka dengan sinar matahari penuh. Pengamatan daya hidup dan pertumbuhan dilakukan pada akhir tahap aklimatisasi dan setelah 2 bulan di area terbuka. Hasil penelitian menunjukkan bahwa eksplan umur 1 minggu yang dikultur pada media padat mempunyai daya hidup tertinggi yakni 83%. Buku tunggal stevia yang dikultur pada medium cair pada tahap hardening selama 4 hari meningkatkan daya hidup menjadi 97% selama aklimatisasi 1 bulan. Setelah aklimatisasi, tinggi tanaman secara rata-rata adalah 2,6 cm dengan 10,6 helai daun. Tanaman yang dipindah ke area terbuka tumbuh pesat dengan tinggi tanaman mencapai 12 cm dengan 30 helai daun dan daya hidup 63% setelah 2 bulan. Hal ini menunjukkan bahwa stevia klon BS 22 mungkin sesuai untuk dataran rendah di daerah tropis. [Kata kunci: tanaman pemanis, eksplan tunas, tahap hardening, daya hidup, tropika] AbstractAcclimatization is a transition period before in vitro culture can be planted in ex vitro environment. In the tropical region, stevia is should be planted at high altitude areas. The development of stevia clones suitable for low land area in the tropics is important to make it possible to apply mechanization in a large scale stevia plantation. The purpose of this research was to determine the effect of explant conditions and hardening period on survival rate and growth during acclimatization stage and early growth of stevia clone B 22 in an open area at low altitude area in the tropics. The first experiment was conducted using different shoot ages: 0, 1, 2 and 3 weeks cultured on solid media as an explant material source. The second experiment was using single node of stevia in different hardening periods in liquid media for 1, 4, 7 and 10 days. Acclimatization was carried out by planting the explants on a mixture growing medium in multi-trays and placed inside a closed plastic tunnel for 1 month. The survival rate and growth parameters were observed at the end of acclimatization stage and after 2 months in the open area. The results show that 1-week explant age on solid media had the highest survival rate at 83%. Hardening single node of shoot in a liquid medium for 4 days increased the survival rate to 97% in 1 month acclimatization stage. After acclimatization, the plant height on average was 2.6 cm with 10.6 leaves. The survived plants planted in an open area grew rapidly to 12 cm in height with 30 leaves and survival rate 63% within 2 months. It indicates that stevia clone BS 22 may suitable for a low altitude area in the tropics. [Key words: sweetener plant, shoot explant, hardening period, survival rate, tropics]

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Section: 7 10mentioning

confidence: 99%

Acclimatization and early growth of tissue culture-derived Stevia rebaudiana at low altitude area in Bogor, Indonesia (Aklimatisasi dan pertumbuhan awal Stevia rebaudiana asal kultur jaringan pada dataran rendah di Bogor, Indonesia)

Sinta¹,

Amanah²

2019

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“…Eksplan yang digunakan pada penelitian ini adalah daun, ruas, dan buku stevia aksenik klon BX koleksi Pusat Penelitian Bioteknologi dan Bioindustri Indonesia (PPBBI), Bogor, Indonesia. Klon ini merupakan salah satu klon unggulan dengan vigor tanaman yang baik, panen 8 kali dalam setahun, produktivitas lapang 4,9 ton daun kering ha -1 tahun -1 serta memiliki kandungan steviosida dan reb A sebesar 10,32% dari berat kering daun (Sinta & Sumaryono, 2019). Induksi kalus dilakukan pada media dasar MS (Murashige & Skoog, 1962) dan sukrosa 3%, gelzan 3,5 gL -1 dengan perlakuan auksin yaitu dengan penambahan 2,4-Diklorofenoksi asetat (2,4-D) 2,5 µM dan asam 3,4-Diklorofenoksi asetat (3,4-D) dengan konsentrasi 1; 2,5 dan 5 µM, media MS tanpa hormon sebagai kontrol.…”

Section: Induksi Kalusunclassified

Inisiasi, pertumbuhan, dan perkembangan kalus embriogenik tanaman stevia (Stevia rebaudiana)

Sinta¹,

Saptari²,

Sumaryono³

2021

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The leaves of sweetener plant Stevia rebaudiana contain secondary metabolites of steviol glycosides which are very sweet, with no calorie and zero glycemic index. Propagation of stevia by seeds is ineffective due to its low germination rate and diverse progenies. The tissue culture of stevia can be used to mass propagate rapidly and is commonly conducted by shoot multiplication. Up to now, the technology of somatic embryogenesis (SE) in stevia has not been successful yet. SE is developed to increase the production scale, rejuvenate clonal-propagated plants, and plant genetic transformation. The research objective was to develop protocols for the initiation, proliferation, and development of embryogenic calli of stevia as potential materials for SE. The explants used were young leaves, nodes, and internodes of axenic plantlets of stevia BX clone. The explants were cultured on MS solid media containing different concentrations of auxin and cytokinin for callus initiation. Callus emerged after 2-3 weeks of culture. The calli obtained were proliferated by subculturing several times as material stocks for indirect SE. MS solid media added with 1 µM 3,4-D and 16 mM CaCl2 gave the highest callus multiplication rate (4.7 times in 3 weeks). The selection of embryogenic calli was made continuously to obtain a pure line of embryogenic calli. Three types of calli attained were friable, fast-growing, yellowish calli, shiny nodular calli, and greenish nodular calli. Histological studies revealed that cells of the nodular calli had been differentiated to potentially formed somatic embryos.

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Observation on local high-yielding stevia (Stevia rebaudiana Bertoni L.) clone specific to Bandung District, West Java

Heliyanto,

Murianingrum,

Hartati

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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The use of stevia (Stevia rebaudiana Bertoni L.) as a natural sweetener shows great potential. However, in Indonesia, the lack of superior and highly productive varieties has been a major challenge in its development. Nukita Co-op has developed a promising stevia clone which we evaluated in this study for its readiness to be proposed as a new and improved variety. Our research was conducted in August 2023 through primary and secondary data collection. Primary data comprised the clone development process and morphological variations observed in farmers’ fields, while secondary data included crop productivity and types of stevia-based products made. Our findings revealed that the CM3 clone, derived from a Vietnamese variety, has been cultivated by farmers in Bandung district since 2010. It has high yields (up to 7-ton dry leaves/ha/year) and is marketed as simplisia (unprocessed ingredients) and various tea variants. However, we noted high morphological variations in farmers’ fields. Therefore, stevia clone CM3 is not ready for direct release. With breeding efforts, this clone could be improved through the selection process and released as a high-yielding variety to support stevia’s development as an alternative sweetener.

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Pertumbuhan, Produksi Biomassa, dan Kandungan Glikosida Steviol pada Lima Klon Stevia Introduksi di Bogor, Indonesia

Cited by 4 publications

References 8 publications

Acclimatization and early growth of tissue culture-derived Stevia rebaudiana at low altitude area in Bogor, Indonesia (Aklimatisasi dan pertumbuhan awal Stevia rebaudiana asal kultur jaringan pada dataran rendah di Bogor, Indonesia)

Acclimatization and early growth of tissue culture-derived Stevia rebaudiana at low altitude area in Bogor, Indonesia (Aklimatisasi dan pertumbuhan awal Stevia rebaudiana asal kultur jaringan pada dataran rendah di Bogor, Indonesia)

Inisiasi, pertumbuhan, dan perkembangan kalus embriogenik tanaman stevia (Stevia rebaudiana)

Observation on local high-yielding stevia (Stevia rebaudiana Bertoni L.) clone specific to Bandung District, West Java

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