Perspectives on avian stem cells for poultry breeding

Kono, Hiroshi

doi:10.1111/asj.12620

Cited by 9 publications

(5 citation statements)

References 57 publications

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“…it has a 15.2 kb negative sense non-segmented RNA genome (ICTV, 2022). NDV can be isolated and propagated in embryonated chicken eggs and antigenically identified by hemagglutination (HA) and hemagglutination inhibition (HI) assays (OIE, 2021;Kagami, 2016). There are many sources for MSCs such as bone marrow, cartilage, and adipose tissue (Li et al, 2018;Peng et al, 2008).…”

Section: Advances Inmentioning

confidence: 99%

Infection of Bone Marrow-Derived Mesenchymal Stem Cells with Virulent Newcastle Disease Virus Maximizes Cytokine Production: A Step Toward vNDV Immunotherapy

Wrshana¹,

Dowidar²,

El-Fiky³

et al. 2022

AAVS

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T he poultry industry represents a cornerstone in the Egyptian economy and a major source of animal protein production. It is associated with a large amount of national consumption that represents over 600 million dollars (FAO, 2013). Excessive usage of antibiotics in poultry has led to the emergence of highly resistant microbial path-ogens (Agyare et al., 2018). So, there is an urgent need to develop environmentally friendly materials and complementary treatments such as natural immune modifying cytokines (Umar et al., 2019).Improvement of meat and egg quality, and disease resistance to viral diseases as Newcastle disease in the Egyptian chicken traits with traditional breeding programs is

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Section: Advances Inmentioning

confidence: 99%

Infection of Bone Marrow-Derived Mesenchymal Stem Cells with Virulent Newcastle Disease Virus Maximizes Cytokine Production: A Step Toward vNDV Immunotherapy

Wrshana¹,

Dowidar²,

El-Fiky³

et al. 2022

AAVS

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“…Due to its high reproductive potential, short intergeneration interval. and embryonic development outside the mother's body, poultry provides unique opportunities for its use in fundamental and applied biological research (Mozdziak & Petitte, 2004;Kagami, 2016) However, the use of the conventional technique for microinjection of foreign DNA into the pronucleus of a fertilized egg, which is well developed for many mammalian species (Gordon & Ruddle, 1981), faces difficulties when applied to birds (Perry, 1988;Love et al, 1994). The creation of a transgenic bird is complicated by the structure of its opaque egg cell with a large yolk and the unique reproductive system of this class.…”

Section: Introductionmentioning

confidence: 99%

Productivity of germinative duck chimaeras and their descendants

Doroshenko¹,

Kostenko²,

Korol³

et al. 2021

animal

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The consequences of chimerization and its possible influence on the productivity of chimera offspring remain poorly understood. The objects of research were ducks (Anas platyrhynchos) of the Shanma (Shan partridge duck) and Shaoxing breeds kept at the Zhuji Guowei Poultry Development Co, Ltd, P.R.China. The study was conducted in the poultry genetics laboratory of the Zhejiang Academy of Agricultural Sciences on a duck farm of Zhejiang Generation Biological Science and Technology Co., Ltd. (Zhejiang Province, PRC). To create chimeras of ducks, the method described by Aige-Gil, Simkiss, 1991; M.T. Tagirov, 2010 was used. Blastodiscs have been isolated from freshly hatched fertilized eggs using a filter paper ring. Shanma duck embryos have been used as recipients, and Shaoxing duck embryos, homozygous for plumage color gene allele (wild type), have been used as donors. Busulfan (SigmaAldrich, United States) have been used as a chemical agent that suppresses a division of primary germ cells (PGC) of recipient embryos. A hole in an eggshell (window) of recipients (Shanma breed) have been made between a blunt and sharp ends of eggs. (This reduced a distance between an injector and an embryo needle). The recipients havebeen incubated for 8–10 hours at a temperature of 38 °C. After recipient eggs incubation for 8 hours, the windows were opened in them. Busulfan was injected into the subgerminal cavity of the embryo with a micropipette (1.5–3 μl of liquid). After busulfan injection, the empty cavity was filled with culture medium (RPMI-1640) supplemented with antibiotics (ampicillin, streptomycin), the hole was closed by plastic wrap and adhesive tape. The eggs have been incubated at a reduced temperature (+32 °C) for 24 hours with the aim of prolong the duration of busulfan action on the PGC (primary germ cells). More than 50% of embryos have been died in the first 2–3 days (after an incubation start). Head and neck disorders have been observed in the 1.2% of embryos. Busulfan injection at a concentration of 300 ng per egg have been leads to 95.0–96.3% mortality of duck embryos, concentration of 150 ng per egg, a mortality rate of 33.3–75.3% have been observed, concentration to 75 ng led to 18.75–38.5% of embryonic mortality. Analysis of the age of puberty (laying of the first egg) indicates that the chimeras matured later. If in the control group the average age of puberty was 139 ± 9 days, in the group of chimeras - 148 ± 13 days. Thus, we can attest that in our experiment, the chimeras matured later than the control animals, which may be due to the effect of busulfan in the sterilization of recipient embryos. The average weight of ducks in the control group was lower, and the group itself was more consolidated. Thus, in the control ducks weighed 1422.40 ± 57.00 g, the chimeras 1608.80 ± 94.76 g. The advantage of live weight chimeras over the control group may be due to the fact that the control group consisted of recipients served by Shanma animals. Egg production of ducks for the entire study period was 87.5 ± 0.05 % (control) 79.5±0.12 % (busulfan). The weight of eggs of ducks of two groups for the entire period was 70.62±0.199 g (control) and 71.15±0.157 g (p˂0.001). The eggs morphometric parameters of the studied ducks groups were: the average values of egg length were 6.056±0.0564 cm (control) and 6.269±0.1341cm (busulfan); egg breadth were 4.520±0.0053 cm (control) and 4.529±0.004 cm (busulfan). There were no statistical intergroup differences in the morphometric parameters of the eggs of the studied groups. In fact, we obtained results similar to the previous ones, which concerned the egg production of daughters of drake chimeras.

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“…This model is a beneficial tool for a wide range of investigations in the field of developmental biology, embryology, toxicity and drug testing, disease modeling, and cell tracking . Separate development of a chick from the mother's body makes these embryos the perfect tool to generate transgenic birds that produce recombinant proteins, create disease‐resistant strains, and protect endangered species . In recent years, a variety of continuous cell lines have been derived from avian embryos such as the duck embryo‐derived EB66 cell line , primordial germ cell (PGCs) , induced pluripotent stem cells (iPSCs) , chicken embryo‐derived PBS‐12F cell line , AGE1.CR , and the quail embryo‐derived QOR2/2E11 cell line .…”

Section: Introductionmentioning

confidence: 99%

Avian embryos and related cell lines: A convenient platform for recombinant proteins and vaccine production

Farzaneh

Hassani

Mozdziak³

et al. 2017

Biotechnology Journal

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Chick embryos are a significant historical research model in basic and applied sciences. The embryonated eggs have been used for virus inoculation in order to vaccine production for nearly a century. Recently, avian eggs and cell lines derived from embryonated eggs have found wide application in biotechnology. This review will discuss about the unique characteristics of avian eggs in terms of safety, large scale and economical production of recombinant proteins. This system also provides the human-like glycosylation on target proteins and therefore can be considered as a suitable host for biomanufacturing of humanized monoclonal antibodies and therapeutic proteins. Avian derived cell lines are an alternative for rapid vaccine manufacturing during a pandemic. Based on the latest knowledge in cell and animal transgenesis, the currently available germ cell-mediated gene transfer system provides a more efficient strategy in gene targeting and creation of transgenic birds that lead to advancements in industrial, biotechnology, and biological research applications. This review covers the recent development of avian fertilized eggs and related cell lines in a variety of human biopharmaceuticals and viral vaccine manufacturing.

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Perspectives on avian stem cells for poultry breeding

Cited by 9 publications

References 57 publications

Infection of Bone Marrow-Derived Mesenchymal Stem Cells with Virulent Newcastle Disease Virus Maximizes Cytokine Production: A Step Toward vNDV Immunotherapy

Infection of Bone Marrow-Derived Mesenchymal Stem Cells with Virulent Newcastle Disease Virus Maximizes Cytokine Production: A Step Toward vNDV Immunotherapy

Productivity of germinative duck chimaeras and their descendants

Avian embryos and related cell lines: A convenient platform for recombinant proteins and vaccine production

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