Perspectives of zebrafish models of epilepsy: What, how and where next?

Stewart, Adam; Desmond, Daniel; Kyzar, Evan J.; Gaikwad, Siddharth; Roth, Andrew; Riehl, Russell; Collins, Christopher; Monnig, Louis; Green, Jeremy; Kalueff, Allan V.

doi:10.1016/j.brainresbull.2011.11.020

Cited by 91 publications

(73 citation statements)

References 149 publications

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“…113,169 Zebrafish display a welldeveloped neuroendocrine system 74,170 and show high sensitivity of their CNS proto-oncogene expression to various experimental manipulations. 87,144,171 From this point of view, the sensitivity of zebrafish physiological biomarkers to hallucinogenic drugs (Table 2) supports the utility of such aquatic models to parallel behavioral phenotypes with physiological responses.…”

Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishmentioning

confidence: 69%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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Among different classes of psychotropic drugs, hallucinogenic agents exert one of the most prominent effects on human and animal behaviors, markedly altering sensory, motor, affective, and cognitive responses. The growing clinical and preclinical interest in psychedelic, dissociative, and deliriant hallucinogens necessitates novel translational, sensitive, and high-throughput in vivo models and screens. Primate and rodent models have been traditionally used to study cellular mechanisms and neural circuits of hallucinogenic drugs' action. The utility of zebrafish (Danio rerio) in neuroscience research is rapidly growing due to their high physiological and genetic homology to humans, ease of genetic manipulation, robust behaviors, and cost effectiveness. Possessing a fully characterized genome, both adult and larval zebrafish are currently widely used for in vivo screening of various psychotropic compounds, including hallucinogens and related drugs. Recognizing the growing importance of hallucinogens in biological psychiatry, here we discuss hallucinogenic-induced phenotypes in zebrafish and evaluate their potential as efficient preclinical models of drug-induced states in humans.

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Section: ■ Modeling Hallucinogenic Drug Action In Zebrafishmentioning

confidence: 69%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

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“…Short-lasting bouts of this behavior represent twitch/spasm behavior. 43 1.17. Bite (biting): Quick movement towards target, with mouth opening and closing, with physical contact.…”

Section: Aggregation Behaviormentioning

confidence: 99%

“…One of the existing challenges in the field is the nonstandardized terminology, when similar phenotypes have different meanings (e.g., circling as part of courtship 42 vs. seizure-like behavior 43 ). Providing necessary distinctions and contexts in such cases is important, better explaining phenomenological and semantic differences which otherwise may be unclear (e.g., to nonspecialists or non-native speakers).…”

Section: Glossarymentioning

confidence: 99%

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

et al. 2013

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Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the growing utility of zebrafish models in this field. Understanding zebrafish behavioral phenotypes provides important insights into neural pathways, physiological biomarkers, and genetic underpinnings of normal and pathological brain function. Novel zebrafish paradigms continue to appear with an encouraging pace, thus necessitating a consistent terminology and improved understanding of the behavioral repertoire. What can zebrafish 'do', and how does their altered brain function translate into behavioral actions? To help address these questions, we have developed a detailed catalog of zebrafish behaviors (Zebrafish Behavior Catalog, ZBC) that covers both larval and adult models. Representing a beginning of creating a more comprehensive ethogram of zebrafish behavior, this effort will improve interpretation of published findings, foster cross-species behavioral modeling, and encourage new groups to apply zebrafish neurobehavioral paradigms in their research. In addition, this glossary creates a framework for developing a zebrafish neurobehavioral ontology, ultimately to become part of a unified animal neurobehavioral ontology, which collectively will contribute to better integration of biological data within and across species.

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“…Epilepsy is a complex brain disorder marked by recurrent spontaneous epileptic seizures associated with typical neurobiological and behavioural alterations [1][2][3]. In the past few decades, rodent seizure models induced by a broad spectrum of mechanisms have contributed significantly to our knowledge on epilepsy and anti-epileptic drugs (AEDs) discovery [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Also, several groups developed a seizure model in zebra-fish larvae and made antiepileptic drugs screening from nature plant [10,17]. However, the major parameters of zebra-fish behavior assay for assessing antiepileptic drug efficacy existed disparity, which resulted in different results in previous studies [3]. Moreover, a question remained as to whether an AED-induced decrease in locomotion is truly indicative of anticonvulsant activity, as some drugs may impair larval movement through other mechanisms such as general toxicity or sedation.…”

Section: Introductionmentioning

confidence: 99%

Validation of the Zebra-fish Pentylene Tetrazol Seizure Model: Behaviour Assay for Assessing Anti-Epileptic Drug Efficacy

Liu¹,

Zhou²

2016

Biochem Anal Biochem

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The availability of zebra-fish larvae model of epileptic seizure provides opportunities to identify novel anticonvulsants for treatment of people with epilepsy. However, the major parameters of zebra-fish behavior assay for assessing antiepileptic drug efficacy existed disparity, which resulted in different results in previous studies. In this study, we chose the high, medium, slow-speed moved distances and the total distances moved for seizure-like activity quantification in individual wells of a 48-well plate in the dark phase and used this zebra-fish seizure model to assess three commonly prescribed anti-epileptic drug's efficacy and screen bioactive components from plant extract. Results showed that the high-speed moved distances were given a more reasonable and sensitive dose-response curve than the total distances in zebra-fish larvae model exposed to 10 mM pentylene tetrazole. Besides, we also optimized the vehicle (dimethyl sulfoxide, DMSO) concentration used in epileptic behaviour assay. The three anti-epileptic drugs, phenytoin, valproate sodium and carbamazepine showed the same efficacy patterns in zebra-fish seizure model as in mammalian epileptic models. We also found four positive hits from plant (Vaccinium spp.) extract in primary drug screening, two hits exhibited concentration-dependent inhibition of locomotor activity, confirming their anticonvulsant characteristics. These results indicated that this zebra-fish larvae model could be useful for assessing anti-epileptic drug efficacy, facilitating the primary drug screening and evaluating of effective components in medicinal plants.

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Perspectives of zebrafish models of epilepsy: What, how and where next?

Cited by 91 publications

References 149 publications

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

Validation of the Zebra-fish Pentylene Tetrazol Seizure Model: Behaviour Assay for Assessing Anti-Epileptic Drug Efficacy

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