Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

Kalueff, Allan V.; Gebhardt, Michael; Stewart, Adam; Cachat, Jonathan; Brimmer, Mallorie; Chawla, Jonathan S; Craddock, Cassandra; Kyzar, Evan J.; Roth, Andrew; Landsman, Samuel; Gaikwad, Siddharth; Robinson, Kyle; Baatrup, Erik; Tierney, Keith B.; Shamchuk, Angela L.; Norton, William; Miller, Noam; Nicolson, Teresa; Braubach, Oliver; Gilman, Charles; Pittman, Julian; Rosemberg, Denis B.; Gerlai, Robert; Echevarria, David J.; Lamb, Elisabeth; Neuhauss, Stephan C.F.; Weng, Wei; Bally‐Cuif, Laure; Schneider, H.

doi:10.1089/zeb.2012.0861

Cited by 866 publications

(708 citation statements)

References 169 publications

(220 reference statements)

Supporting

Mentioning

689

Contrasting

Unclassified

Order By: Relevance

“…We observed no behavioural differences between groups during exposure to the shock: all groups showed erratic movements, seeking to escape or jumping out of the water when the shock was applied (Manuel et al, 2014). In addition, we did not observe any abnormal behaviour, such as periods of freezing behaviour (Kalueff et al, 2013), on any of the test days.…”

Section: General Observationsmentioning

confidence: 55%

Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase

Manuel

Gorissen

Zethof

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

Zebrafish (Danio rerio Hamilton) are increasingly used as a model to study the effects of chronic stress on brain and behaviour. In rodents, unpredictable chronic stress (UCS) has a stronger effect on physiology and behaviour during the active phase than during the resting phase. Here, we applied UCS during the daytime (active phase) for 7 and 14 days or during the night-time (resting phase) for 7 nights in an in-house-reared Tuebingen long-fin (TLF) zebrafish strain. Following UCS, inhibitory avoidance learning was assessed using a 3 day protocol where fish learn to avoid swimming from a white to a black compartment where they will receive a 3 V shock. Latencies of entering the black compartment were recorded before training (day 1; first shock) and after training on day 2 (second shock) and day 3 (no shock, tissue sampling). Fish whole-body cortisol content and expression levels of genes related to stress, fear and anxiety in the telencephalon were quantified. Following 14 days of UCS during the day, inhibitory avoidance learning decreased (lower latencies on days 2 and 3); minor effects were found following 7 days of UCS. Following 7 nights of UCS, inhibitory avoidance learning decreased (lower latency on day 3). Whole-body cortisol levels showed a steady increase compared with controls (100%) from 7 days of UCS (139%), to 14 days of UCS (174%) to 7 nights of UCS (231%), suggestive of an increasing stress load. Only in the 7 nights of UCS group did expression levels of corticoid receptor genes (mr, grα, grβ) and of bdnf increase. These changes are discussed as adaptive mechanisms to maintain neuronal integrity and prevent overload, and as being indicative of a state of high stress load. Overall, our data suggest that stressors during the resting phase have a stronger impact than during the active phase. Our data warrant further studies on the effect of UCS on stress axis-related genes, especially grβ; in mammals this receptor has been implicated in glucocorticoid resistance and depression.

show abstract

Section: General Observationsmentioning

confidence: 55%

Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase

Manuel

Gorissen

Zethof

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…46 Collectively, this renders zebrafish models particularly suitable for drug discovery and in vivo screening of hallucinogens and related compounds (see ref 64 for a comprehensive catalog of hallucinogenic drug-evoked behaviors in zebrafish).…”

Section: ■ Developing Complementary Nonmammalian Models For Hallucinomentioning

confidence: 99%

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Neelkantan

Mikhaylova

Stewart

et al. 2013

ACS Chem. Neurosci.

Self Cite

View full text Add to dashboard Cite

Among different classes of psychotropic drugs, hallucinogenic agents exert one of the most prominent effects on human and animal behaviors, markedly altering sensory, motor, affective, and cognitive responses. The growing clinical and preclinical interest in psychedelic, dissociative, and deliriant hallucinogens necessitates novel translational, sensitive, and high-throughput in vivo models and screens. Primate and rodent models have been traditionally used to study cellular mechanisms and neural circuits of hallucinogenic drugs' action. The utility of zebrafish (Danio rerio) in neuroscience research is rapidly growing due to their high physiological and genetic homology to humans, ease of genetic manipulation, robust behaviors, and cost effectiveness. Possessing a fully characterized genome, both adult and larval zebrafish are currently widely used for in vivo screening of various psychotropic compounds, including hallucinogens and related drugs. Recognizing the growing importance of hallucinogens in biological psychiatry, here we discuss hallucinogenic-induced phenotypes in zebrafish and evaluate their potential as efficient preclinical models of drug-induced states in humans.

show abstract

“…To this aim, we embedded HuC:GCaMP5g larvae in agarose such that the tail was free to move and chose a 5× objective to obtain a field of view covering the entire larva. After cadaverine delivery, evoked neuronal calcium fluxes were recorded while concurrent tail movements were detected that are indicative of avoidance responses 21 [ Fig. 2(d)].…”

Section: Resultsmentioning

confidence: 99%

Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera

et al. 2015

View full text Add to dashboard Cite

Abstract. Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond

Cited by 866 publications

References 169 publications

Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase

Unpredictable chronic stress decreases inhibitory avoidance learning in Tuebingen Long-Fin zebrafish (Danio rerio Hamilton): stronger effects in the resting phase than in the active phase

Perspectives on Zebrafish Models of Hallucinogenic Drugs and Related Psychotropic Compounds

Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera

Contact Info

Product

Resources

About