Perspective: Emerging strategies for determining atomic-resolution structures of macromolecular complexes within cells

Concluding remarks: Challenges and future developments in biological electron cryo-microscopy

“…Some of these developments are already in hand and likely to come to fruition within a year or two. They include electron-optical correctors of chromatic aberration, known as C c correctors; 30 the laser phase plate; 31 and cooling the specimen with liquid helium instead of liquid nitrogen. 32 C c correctors focus inelastically scattered electrons which are otherwise removed by an energy filter.…”