Concluding remarks: Challenges and future developments in biological electron cryo-microscopy

Kühlbrandt, Werner

doi:10.1039/d2fd90062a

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…Cryo EM technology, as a powerful tool in biomedical research, has shown great potential in the field of protein and macromolecular complex structure analysis. However, in the pursuit of deeper understanding of biomolecules, Cryo EM still faces many challenges, especially in sample preparation, data collection and processing (Kühlbrandt, 2022). The difficulty of sample preparation, especially in rapidly freezing samples to preserve their structure close to the original ecology without forming ice crystals, and finding suitable preparation methods for proteins that are difficult to express or stable, is a major challenge.…”

Section: Challenges and Prospectsmentioning

confidence: 99%

The Latest Progress of Cryo Electron Microscopy Technology in Protein Structure Analysis

Wang

2024

gab

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With the rapid development of Cryo Electron Microscopy (Cryo EM) technology, significant progress has been made in protein structure analysis, opening up new avenues for research in the fields of biology and medicine. This study aims to review the latest progress of cryo electron microscopy technology in protein structure analysis, and explore its research significance and application prospects. Through in-depth analysis of technological optimization, method innovation, and its application in the study of complex biological macromolecular structures, it was found that cryoelectron microscopy technology not only improves resolution and signal-to-noise ratio, but also successfully analyzes various complex protein structures, providing powerful tools for research in the fields of biology and medicine. The development of cryoelectron microscopy technology not only deepens the understanding of protein structure and function relationships, but also provides new ideas and methods for drug development and disease treatment. Therefore, further promoting the development and application of cryoelectron microscopy technology is of great significance for promoting progress in the field of life sciences.

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Section: Challenges and Prospectsmentioning

confidence: 99%

The Latest Progress of Cryo Electron Microscopy Technology in Protein Structure Analysis

Wang

2024

gab

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The Technology in Cryotechnology

Hagedorn,

Alkurdi,

Barker

et al. 2023

cryo letters

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The process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or assisted fertilization. In this work we provide an overview of various technologies in the field of cryotechnology with regard to the freezing, storage and thawing of living cells. The first part covers the freezing process, starting with cryoprotective agents regarding their protection mechanisms and compositions, passing by cryoimaging, micro-fluidic systems, and the currently available freezing and biobanking equipment. The second part focusses on the thawing process as well as the hypothermic preservation for the short-term storage of biological materials and constructs.

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Concluding remarks: Challenges and future developments in biological electron cryo-microscopy

Abstract: CryoEM has made amazing progress during the past 10 years and is in a process of rapid transformation. This year’s Faraday Discussions examined some of the remaining challenges.

Cited by 2 publications

References 36 publications

The Latest Progress of Cryo Electron Microscopy Technology in Protein Structure Analysis

The Latest Progress of Cryo Electron Microscopy Technology in Protein Structure Analysis

The Technology in Cryotechnology

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