Perspective and Consensus Opinion: Good Practices for Using Organotypic Skin and Epidermal Equivalents in Experimental Dermatology Research

Abstract

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Skin sensitization to fragrance hydroperoxides: interplay between dendritic cells, keratinocytes and free radicals

“…23 As recommended, we tested RHE covering three donors. 35 Data presented in Figure 2b show clear upregulation of CD86 and CD54 on THP-1 when 120 µg cm À2 (1Á2%) limonene-1-OOH or 200 µg cm À2 limonene-2-OOH was applied, while no upregulation or only marginal upregulation was observed for 20 µg cm À2 (0Á2%).…”

New Perspectives in the Antimicrobial Activity of the Amphibian Temporin B: Peptide Analogs Are Effective Inhibitors of Candida albicans Growth

“…Based on our results, both peptides can be categorized as "non-irritant compounds". It has to be evaluated in future studies if the peptides hold promise in their efficacy to reduce or eradicate the fungal load in full thickness infection models, which possess also a dermal component and can replicate epithelial-mesenchymal signaling in response to diverse stimuli or in appropriate in vivo models [49].…”

Tight junctions in the skin: still a lot to learn

“…In this issue of the BJD, Sulaimani et al investigate the effects of DMF on various subsets of T cells in vivo and demonstrated its potential direct effects on Th17 and Treg cell populations, before providing a mechanistic analysis in vitro. 7 Flow cytometric analysis demonstrated that DMF treatment in patients with psoriasis induced a significant reduction in the total number of CD45RO + memory CD4 and CD8 T cells. This confirms that lymphocytopenia is one of the most frequent side-effects of DMF.…”

“…Nevertheless, they provide a good model for initial investigation that must be validated in more complex systems and in vivo. The challenge for the field is to establish a model, 7 or potentially more than one depending on the outcomes, that truly reassembles the complexity of TJs in the epidermis, while still being accessible and affordable.…”