Persistent impaired glucose metabolism in a zebrafish hyperglycemia model

Capiotti, Katiúcia Marques; Antonioli, Régis; Kist, Luiza Wilges; Bogo, Maurı́cio Reis; Bonan, Carla Denise; Silva, Rosane Souza da

doi:10.1016/j.cbpb.2014.03.005

Cited by 122 publications

(106 citation statements)

References 39 publications

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“…After, we performed the evaluation of the E-NTPDases, ada-related, and adora gene expressions from encephala of adult zebrafish. The blood glucose levels from glucose-treated and washout animals demonstrated the same profile already developed in previous works [8,42]. Nucleotides are important extracellular messengers in both physiological and pathological conditions [11].…”

Section: Discussionsupporting

confidence: 76%

“…Our results demonstrated that ecto-ADA activity was significantly increased in 111-mM glucose group, suggesting a decrease in adenosine levels in synaptic cleft. There is some consensus that a decrease of adenosine levels could be related with cognitive impairment observed in diabetic rats [8,55,56], which also could be suggested occurring in the zebrafish hyperglycemic model.…”

Section: Discussionmentioning

confidence: 97%

“…Groups of 15 adult animals were placed in 5-L aquariums containing 111 mM glucose solution, which were diluted in water and maintained during 14 days at room temperature as described in previous section according to Capiotti et al (2014a) [8]. The solutions of glucose were exchanged three times per week to avoid contamination with opportunistic microorganisms.…”

Section: Hyperglycemia Modelmentioning

confidence: 99%

“…In order to verify if the effects caused by 111-mM glucose solution treatment would be persistent, a group of 15 fish was kept in glucose-free water by additional 7 days (washout group, no glucose added) [8]. During this period, the animals were maintained at the same conditions of welfare described in previous section.…”

Section: Withdrawal Of Glucosementioning

confidence: 99%

“…DM is a chronic disease, characterized by hyperglycemia, resulting from defects in insulin secretion, insulin action, or both [2,3]. The effects of this disease are being strongly associated with neurophysiological, neurochemical, and behavioral modifications in brain of patients that are also observed in animal model of DM [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

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Hyperglycemia alters E-NTPDases, ecto-5′-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio)

Capiotti

Siebel

Kist

et al. 2016

Purinergic Signalling

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Hyperglycemia is the main feature for the diagnosis of diabetes mellitus (DM). Some studies have demonstrated the relationship between DM and dysfunction on neurotransmission systems, such as the purinergic system. In this study, we evaluated the extracellular nucleotide hydrolysis and adenosine deamination activities from encephalic membranes of hyperglycemic zebrafish. A significant decrease in ATP, ADP, and AMP hydrolyses was observed at 111-mM glucose-treated group, which returned to normal levels after 7 days of glucose withdrawal. A significant increase in ectoadenosine deaminase activity was observed in 111-mM glucose group, which remain elevated after 7 days of glucose withdrawal. The soluble-adenosine deaminase activity was significantly increased just after 7 days of glucose withdrawal. We also evaluated the gene expressions of ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases), ecto-5′-nucleotidase, ADA, and adenosine receptors from encephala of adult zebrafish. The entpd 2a.1, entpd 2a.2, entpd 3, and entpd 8 mRNA levels from encephala of adult zebrafish were decreased in 111-mM glucose-treated and glucose withdrawal groups. The gene expressions of adenosine receptors (adora 1 , adora 2aa , adora 2ab , and adora 2b ) were decreased in 111-mM glucose-treated and glucose withdrawal groups. The gene expression of ADA (ada 2a.1) was decreased in glucose withdrawal group. Maltodextrin, used as a control, did not affect the expression of adenosine receptors, ADA and E-NTPDases 2, 3, and 8, while the expression of ecto-5′-nucleotidase was slightly increased and the E-NTPDases 1 decreased. These findings demonstrated that hyperglycemia might affect the ecto-nucleotidase and adenosine deaminase activities and gene expression in zebrafish, probably through a mechanism involving the osmotic effect, suggesting that the modifications caused on purinergic system may also contribute to the diabetes-induced progressive cognitive impairment.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 97%

Section: Hyperglycemia Modelmentioning

confidence: 99%

Section: Withdrawal Of Glucosementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hyperglycemia alters E-NTPDases, ecto-5′-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio)

Capiotti

Siebel

Kist

et al. 2016

Purinergic Signalling

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Impaired Detoxification of Trans, Trans‐2,4‐Decadienal, an Oxidation Product from Omega‐6 Fatty Acids, Alters Insulin Signaling, Gluconeogenesis and Promotes Microvascular Disease

Qian,

Klatt,

Bennewitz

et al. 2023

Advanced Science

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Omega‐6 fatty acids are the primary polyunsaturated fatty acids in most Western diets, while their role in diabetes remains controversial. Exposure of omega‐6 fatty acids to an oxidative environment results in the generation of a highly reactive carbonyl species known as trans, trans‐2,4‐decadienal (tt‐DDE). The timely and efficient detoxification of this metabolite, which has actions comparable to other reactive carbonyl species, such as 4‐hydroxynonenal, acrolein, acetaldehyde, and methylglyoxal, is essential for disease prevention. However, the detoxification mechanism for tt‐DDE remains elusive. In this study, the enzyme Aldh9a1b is identified as having a key role in the detoxification of tt‐DDE. Loss of Aldh9a1b increased tt‐DDE levels and resulted in an abnormal retinal vasculature and glucose intolerance in aldh9a1b−/− zebrafish. Transcriptomic and metabolomic analyses revealed that tt‐DDE and aldh9a1b deficiency in larval and adult zebrafish induced insulin resistance and impaired glucose homeostasis. Moreover, alterations in hyaloid vasculature is induced by aldh9a1b knockout or by tt‐DDE treatment can be rescued by the insulin receptor sensitizers metformin and rosiglitazone. Collectively, these results demonstrated that tt‐DDE is the substrate of Aldh9a1b which causes microvascular damage and impaired glucose metabolism through insulin resistance.

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Developments in the study of Chinese herbal medicine's assessment index and action mechanism for diabetes mellitus

Liu,

Zheng,

Wang

et al. 2024

Anim Models and Exp Med

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In traditional Chinese medicine (TCM), based on various pathogenic symptoms and the ‘golden chamber’ medical text, Huangdi Neijing, diabetes mellitus falls under the category ‘collateral disease’. TCM, with its wealth of experience, has been treating diabetes for over two millennia. Different antidiabetic Chinese herbal medicines reduce blood sugar, with their effective ingredients exerting unique advantages. As well as a glucose lowering effect, TCM also regulates bodily functions to prevent diabetes associated complications, with reduced side effects compared to western synthetic drugs. Chinese herbal medicine is usually composed of polysaccharides, saponins, alkaloids, flavonoids, and terpenoids. These active ingredients reduce blood sugar via various mechanism of actions that include boosting endogenous insulin secretion, enhancing insulin sensitivity and adjusting key enzyme activity and scavenging free radicals. These actions regulate glycolipid metabolism in the body, eventually achieving the goal of normalizing blood glucose. Using different animal models, a number of molecular markers are available for the detection of diabetes induction and the molecular pathology of the disease is becoming clearer. Nonetheless, there is a dearth of scientific data about the pharmacology, dose‐effect relationship, and structure–activity relationship of TCM and its constituents. Further research into the efficacy, toxicity and mode of action of TCM, using different metabolic and molecular markers, is key to developing novel TCM antidiabetic formulations.

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Persistent impaired glucose metabolism in a zebrafish hyperglycemia model

Cited by 122 publications

References 39 publications

Hyperglycemia alters E-NTPDases, ecto-5′-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio)

Hyperglycemia alters E-NTPDases, ecto-5′-nucleotidase, and ectosolic and cytosolic adenosine deaminase activities and expression from encephala of adult zebrafish (Danio rerio)

Impaired Detoxification of Trans, Trans‐2,4‐Decadienal, an Oxidation Product from Omega‐6 Fatty Acids, Alters Insulin Signaling, Gluconeogenesis and Promotes Microvascular Disease

Developments in the study of Chinese herbal medicine's assessment index and action mechanism for diabetes mellitus

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