Persistence of Staphylococcus aureus colonization on the skin of NC/Nga mice

Hashimoto, Yuki; Kaneda, Yoshie; Akashi, Toshi; Arai, Iwao; Nakaike, Shiro

doi:10.1016/j.jdermsci.2004.04.003

Cited by 14 publications

(15 citation statements)

References 30 publications

(33 reference statements)

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“…6B). Consistent with the known transient nature of S. aureus colonization in immune competent mice (Hashimoto et al, 2004), we observed that by day 4 the bioluminescence signals were quite low (Fig. 6C), and by day 5 had reached baseline, indicating that bacterial clearance had occurred.…”

Section: Resultssupporting

confidence: 85%

Coagulase-Negative Staphylococcal Strain Prevents Staphylococcus aureus Colonization and Skin Infection by Blocking Quorum Sensing

Paharik

Parlet

Chung

et al. 2017

Cell Host & Microbe

178

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Summary Coagulase-negative staphylococci (CoNS) and Staphylococcus aureus are part of the natural flora of humans and other mammals. We found that spent media from the CoNS species Staphylococcus caprae can inhibit agr-mediated quorum sensing by all classes of S. aureus. A biochemical assessment of the inhibitory activity suggested that the S. caprae autoinducing peptide (AIP) was responsible, and mass spectrometric analysis identified the S. caprae AIP as an eight-residue peptide (YSTCSYYF). Using a murine model of intradermal MRSA infection, the therapeutic efficacy of synthetic S. caprae AIP was evident by a dramatic reduction in both dermonecrotic injury and cutaneous bacterial burden relative to controls. Competition experiments between S. caprae and MRSA demonstrated a significant reduction in MRSA burden using murine models of both skin colonization and intradermal infection. Our findings indicate that important interactions occur between commensals that can impact disease outcomes and potentially shape the composition of the natural flora.

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Section: Resultssupporting

confidence: 85%

Coagulase-Negative Staphylococcal Strain Prevents Staphylococcus aureus Colonization and Skin Infection by Blocking Quorum Sensing

Paharik

Parlet

Chung

et al. 2017

Cell Host & Microbe

178

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“…NC/Nga mice with AD-like skin lesions show IgE hyperproduction in serum, severe scratching behaviour and greatly elevated colonization of S. aureus in the skin. 16,17,19,22,23 In the present study, the control group of mice also developed skin symptoms as described above.…”

Section: Discussionsupporting

confidence: 52%

“…Scratching their skin with the toenails appeared to be an important factor in progression to dermatitis by increasing the intensity of various immunological responses, such as serum IgE elevation and increases in the numbers of mast cells. 30,31 In addition, numbers of S. aureus on the skin were found to coincide with the severity of dermatitis in NC/Nga mice, 23 and clarithromycin, a macrolide antibiotic, inhibited the development of dermatitis in NC/Nga mice. 32 In the present study, the administration of RJH-E inhibited scratching behaviour and S. aureus colonization, which may contribute to reduce various inflammatory and immunological responses.…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice

et al. 2006

British Journal of Dermatology

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“…Therefore, mechanisms of colonization on tissues such as skin may be quite different from what occurs on anterior nares. In this study, we performed a murine ear colonization experiment (18) to understand the mechanism of skin colonization by employing MRSA strain MW2 (1). The strain, representing community-acquired MRSA (CA-MRSA), is considered a highly virulent MRSA strain with a great ability to colonize human tissue.…”

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confidence: 99%

Beta-Hemolysin Promotes Skin Colonization by Staphylococcus aureus

Katayama

Baba

Sekine

et al. 2013

Journal of Bacteriology

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Colonization by Staphylococcus aureus is a characteristic feature of several inflammatory skin diseases and is often followed by epidermal damage and invasive infection. In this study, we investigated the mechanism of skin colonization by a virulent community-acquired methicillin-resistant S. aureus (CA-MRSA) strain, MW2, using a murine ear colonization model. MW2 does not produce a hemolytic toxin, beta-hemolysin (Hlb), due to integration of a prophage, Sa3mw, inside the toxin gene (hlb). However, we found that strain MW2 bacteria that had successfully colonized murine ears included derivatives that produced Hlb. Genome sequencing of the Hlb-producing colonies revealed that precise excision of prophage Sa3mw occurred, leading to reconstruction of the intact hlb gene in their chromosomes. To address the question of whether Hlb is involved in skin colonization, we constructed MW2-derivative strains with and without the Hlb gene and then subjected them to colonization tests. The colonization efficiency of the Hlb-producing mutant on murine ears was more than 50-fold greater than that of the mutant without hlb. Furthermore, we also showed that Hlb toxin had elevated cytotoxicity for human primary keratinocytes. Our results indicate that S. aureus Hlb plays an important role in skin colonization by damaging keratinocytes, in addition to its well-known hemolytic activity for erythrocytes.

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Persistence of Staphylococcus aureus colonization on the skin of NC/Nga mice

Cited by 14 publications

References 30 publications

Coagulase-Negative Staphylococcal Strain Prevents Staphylococcus aureus Colonization and Skin Infection by Blocking Quorum Sensing

Coagulase-Negative Staphylococcal Strain Prevents Staphylococcus aureus Colonization and Skin Infection by Blocking Quorum Sensing

Inhibitory effects of Rumex japonicus Houtt. on the development of atopic dermatitis-like skin lesions in NC/Nga mice

Beta-Hemolysin Promotes Skin Colonization by Staphylococcus aureus

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