Persistence of monkeypox virus DNA in clinical specimens

Beyond stigma: Monkeypox infection in a 27‐year‐old woman

“…6,7 The long healing time of the genital lesion could be due to the anatomical characteristics of the vulva, as a higher humidity of the mucosa do not promote a rapid healing process. 8 Additionally, in this case a high MPX-DNA load was detected at baseline evaluation (C t = 13) with MPX-DNA persistence on the vulvar lesion but not in plasma, after 10 Although the male partner's source of infection could not be established despite an in-depth case interview, sexual history should thoroughly be assessed among suspected cases. 9 Moreover, considering the ongoing MPX vaccination campaign which, in Italy, is currently recommended to MSM with known riskfactors, 10…”

The recent re-emergence of human monkeypox: Would it become endemic beyond Africa?

“…Monkeypox virus could also be transmitted via sexual contact [41] . Monkeypox virus DNA has been detected in rectal swabs, semen, and feces, with the median time of persistence of 8.3, 11.4, and 9.4, respectively; thus, increasing the likelihood of acquisition through sexual contacts [42] , [43] . In a case report, it was reported that genital rash occurred at 5 days after sexual contact, followed by fever and lymphadenopathy at 3 days after rash onset [44] .…”

Assessment of Urinary Biomarkers for Infectious Diseases Using Lateral Flow Assays: A Comprehensive Overview

“…68 The most recent monkeypox viral deoxyribonucleic acid (DNA), a biomarker for monkeypox virus (MPXV) infection was detected in different body fluid samples like semen, blood, and skin lesions, but was reported to be most stable in urine samples. 69 In such cases, a POC test, NALFA with enhanced sensitivity, can be ventured for detection of MPVX DNA from clinically suspected urine samples. A shorter detection time can improve adoption of preventive measures like quarantine and medication, thereby limiting transmission of infections.…”

“…Lack of diagnostic preparedness has significantly delayed identification of outbreaks for multiple pathogens, including Ebola, Lassa fever, yellow fever, and Zika, majorly due to poor primary care facilities . The most recent monkeypox viral deoxyribonucleic acid (DNA), a biomarker for monkeypox virus (MPXV) infection was detected in different body fluid samples like semen, blood, and skin lesions, but was reported to be most stable in urine samples . In such cases, a POC test, NALFA with enhanced sensitivity, can be ventured for detection of MPVX DNA from clinically suspected urine samples.…”