Persistence and genetic diversity among strains of phycoerythrinrich cyanobacteria from the picoplankton of Lake Constance

Postius, Christine; Ernst, Anneliese; Kenter, Ulrich; Böger, Peter

doi:10.1093/plankt/18.7.1159

Cited by 34 publications

(35 citation statements)

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“…PCR products of the first reaction, conducted with primers targeting 16S rDNA and 23S rDNA, were not visible on agarose gel (data not shown). However, DGGE analysis of the short GC-clamped fragments produced from these templates in a nested PCR confirmed the high diversity of PE-rich strains expected from the low redundancy of isolation of single genotypes (30). Amplicons with similar melting behaviors to those of strains BO 8808, BO 8809, and BO 9402, which could not be separated on our gels, dominated in all samples collected in 1999 and 2000 ( Fig.…”

Section: Resultssupporting

confidence: 61%

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Quantitative Tracing, byTaqNuclease Assays, of aSynechococcusEcotype in a Highly Diversified Natural Population

Becker

Fahrbach

Böger

et al. 2002

Appl Environ Microbiol

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101

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Quantitative Taq nuclease assays (TNAs) (TaqMan PCR), nested PCR in combination with denaturing gradient gel electrophoresis (DGGE), and epifluorescence microscopy were used to analyze the autotrophic picoplankton (APP) of Lake Constance. Microscopic analysis revealed dominance of phycoerythrin (PE)-rich Synechococcus spp. in the pelagic zone of this lake. Cells passing a 3-m-pore-size filter were collected during the growth period of the years 1999 and 2000. The diversity of PE-rich Synechococcus spp. was examined using DGGE to analyze GC-clamped amplicons of a noncoding section of the 16S-23S intergenic spacer in the ribosomal operon. In both years, genotypes represented by three closely related PE-rich Synechococcus strains of our culture collection dominated the population, while other isolates were traced sporadically or were not detected in their original habitat by this method. For TNAs, primer-probe combinations for two taxonomic levels were used, one to quantify genomes of all known Synechococcus-type cyanobacteria in the APP of Lake Constance and one to enumerate genomes of a single ecotype represented by the PE-rich isolate Synechococcus sp. strain BO 8807. During the growth period, genome numbers of known Synechococcus spp. varied by 2 orders of magnitude (2.9 ؋ 10 3 to 3.1 ؋ 10 5 genomes per ml). The ecotype Synechococcus sp. strain BO 8807 was detected in every sample at concentrations between 1.6 ؋ 10 1 and 1.3 ؋ 10 4 genomes per ml, contributing 0.02 to 5.7% of the quantified cyanobacterial picoplankton. Although the quantitative approach taken in this study has disclosed several shortcomings in the sampling and detection methods, this study demonstrated for the first time the extensive internal dynamics that lie beneath the seemingly arbitrary variations of a population of microbial photoautotrophs in the pelagic habitat.

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Section: Resultssupporting

confidence: 61%

“…Synechococcus spp. (strains BO 8807, BO 8808, BO 8809, BO 9101, BO 9402, BO 9403, and BO 9404) were isolated from the pelagic zone of Lake Constance (Bodensee) (4,30) and were cultured in 40 ml of the mineral liquid medium BG11 (34) under low light intensity between 5 and 10 microeinsteins m Ϫ2 s Ϫ1 . Sampling.…”

Section: Methodsmentioning

confidence: 99%

Quantitative Tracing, byTaqNuclease Assays, of aSynechococcusEcotype in a Highly Diversified Natural Population

Becker

Fahrbach

Böger

et al. 2002

Appl Environ Microbiol

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101

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“…Base ambiguities detected in both strands were observed in ITS-1 of strain BO 9403 and in two strains isolated from the Bornholm Sea (BS 5 and BS 6). On the other hand, strains with identical RFLP of psbA genes, such as the two PE-rich strains BO 8807 and BO 8810 or the three PC-rich strains BO 9301, BO 9302 and BO 9303 (Ernst et al, 1995;Postius et al, 1996), exhibited sequence identity in ITS-1 (data not shown).…”

Section: Resultsmentioning

confidence: 97%

“…Physiological studies showed that apart from the obvious difference in the major accessory pigments, PC and PE, the strains also differed in carotenoid content, light stress tolerance, response to nutrient limitation and surface structures (for review see Postius & Ernst, 1999). In addition, most freshwater isolates have previously been characterized as distinct genotypes by a genomic fingerprinting technique, RFLP of psbA genes (Ernst et al, 1995;Postius et al, 1996). However, the phylogenetic relationships of these strains have not been examined.…”

Section: Introductionmentioning

confidence: 99%

“…Two to three weeks after inoculation of 40 ml batches containing BG11, cultures were harvested by centrifugation (7 min, 7000 g), frozen in liquid nitrogen and stored at 220˚C. Genomic DNA was extracted according to Postius et al (1996). For RFLP analysis, 5-10 mg genomic DNA was completely digested with different restriction endonucleases (Boehringer), separated by agarose gel electrophoresis and blotted on Hybond (Amersham) membranes (Ernst et al, 1995).…”

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Ecosystem-dependent adaptive radiations of picocyanobacteria inferred from 16S rRNA and ITS-1 sequence analysis

et al. 2003

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Small, coccoid and rod-shaped Synechococcus-type cyanobacteria with either phycoerythrin or phycocyanin as major accessory pigments were isolated from several large, temperate-zone lakes and the brackish Baltic Sea. The picocyanobacteria had two ribosomal operons with a long internal transcribed spacer (ITS-1) separating the 16S rDNA and 23S rDNA. A 16S rRNA-based phylogenetic analysis assigned all isolates to the picophytoplankton clade [sensu Urbach, E., 46,[188][189][190][191][192][193][194][195][196][197][198][199][200][201], which also comprises marine Synechococcus spp. and Prochlorococcus spp. The strains assorted to five paraphyletic clusters each containing two or more strains with 99?4-100 % 16S rRNA sequence identity. Five corresponding strain clusters were deduced from analysis of ITS-1 sequences. Sequence divergence in ITS-1 varied between 23 % in the most divergent and 1 % in the phylogenetically most conserved cluster. Clustered strains with low sequence divergence in ITS-1 were frequently isolated from a single ecosystem or hydrographically comparable lakes in the same region. They represent physiologically distinct ecotypes of species which, among other phenotypic variations, frequently differed in their major accessory pigments, the phycobiliproteins. The reproduction of the various pigment traits in different lineages was not correlated with the phylogenetic divergence deduced from 16S rRNA or ITS-1 sequences but rather seemed to be related to characteristics of the ecosystem and habitat from which the strains were isolated. The occurrence of a comparable spectrum of phenotypes in different lineages and ecosystems indicates that different strain clusters developed similar ecotypes during independent adaptive radiations.

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Picoplankton, Freshwater

Callieri

Stockner

2003

Encyclopedia of Environmental Microbiology

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Persistence and genetic diversity among strains of phycoerythrinrich cyanobacteria from the picoplankton of Lake Constance

Cited by 34 publications

References 20 publications

Quantitative Tracing, byTaqNuclease Assays, of aSynechococcusEcotype in a Highly Diversified Natural Population

Quantitative Tracing, byTaqNuclease Assays, of aSynechococcusEcotype in a Highly Diversified Natural Population

Ecosystem-dependent adaptive radiations of picocyanobacteria inferred from 16S rRNA and ITS-1 sequence analysis

Picoplankton, Freshwater

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