Ecosystem-dependent adaptive radiations of picocyanobacteria inferred from 16S rRNA and ITS-1 sequence analysis

Ernst, Anneliese; Becker, Sven; Wollenzien, Ute; Postius, Christine

doi:10.1099/mic.0.25475-0

Cited by 143 publications

(200 citation statements)

References 57 publications

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“…The fourth and largest group contains 39 strains and is designated the Baltic Sea group (Bsea; Figure 2). The 16S rRNA-ITS-1 sequences of these strains show the highest similarity to those of Synechococcus WH5701 and BO8805 that belong to the marine cluster B or the subalpine cluster II (Ernst et al, 2003;Chen et al, 2006). The 16S rRNA-ITS sequences of BSea isolates can be completely aligned with WH5701 and BO8805.…”

Section: Molecular Identification Of the Baltic Sea Picocyanobacteriamentioning

confidence: 71%

“…Synechococcus species are phylogenetically divided into several major clusters. Picocyanobacteria that are often found and isolated from marine, brackish and freshwater environments are related to Synechococcus cluster 5 (Herdman et al, 2001;Crosbie et al, 2003;Ernst et al, 2003). At the 16S rRNA level, this cluster is closely related to the sister groups Prochlorococcus and Cyanobium.…”

Section: Introductionmentioning

confidence: 99%

“…The 16S rRNA-ITS-1 sequence of strain CCY0426 clustered with strains belonging to group A of the picocyanobacteria (Ernst et al, 2003;Chen et al, 2006). Group A is also known as the Cyanobium gracile cluster (Ernst et al, 2003). The second group comprises the strains CCY0420, CCY0454 and CCY0470.…”

Section: Molecular Identification Of the Baltic Sea Picocyanobacteriamentioning

confidence: 99%

“…The second group comprises the strains CCY0420, CCY0454 and CCY0470. Their 16S rRNA-ITS-1 sequences form a monophyletic clade with CCY9201 (former BS4) and CCY9202 (former BS5) that were previously isolated from the Baltic Sea (Ernst et al, 2003;Stomp et al, 2004) and were assigned as 'Bornholm Sea Group'. The third clade comprises the ITS-1 sequences of the strains CCY0432, CCY0468 and CCY0490.…”

Section: Molecular Identification Of the Baltic Sea Picocyanobacteriamentioning

confidence: 99%

“…The 16S-ITS operon was amplified using the primers B1055F and PitsEcyanR (Ernst et al, 2003;Zaballos et al, 2006), whereas in a separate reaction the cpcBA operon was amplified using the primers cpcBF (UFP)/ cpcAR (URP) (Robertson et al, 2001). For each PCR reaction, the mixture contained 0.625 units of HotStarTaq (Qiagen, Venlo, The Netherlands), 0.2 mM of each dNTP (Roche, Woerden, The Netherlands), 1 Â PCR buffer (Qiagen), 5 pmol of the forward and reverse primers were used per reaction and purified Milli-Q-Grade (MQ-grade) water (Millipore purification unit, 18 MO Á cm (Millipore, Billerica, MA, USA)) was added to a final volume of 25 ml.…”

Section: Pcr Reactionsmentioning

confidence: 99%

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Colorful microdiversity of Synechococcus strains (picocyanobacteria) isolated from the Baltic Sea

et al. 2008

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Synechococcus is a cosmopolitan genus of picocyanobacteria living in the photic zone of freshwater and marine ecosystems. Here, we describe the isolation of 46 closely related picocyanobacterial strains from the Baltic Sea. The isolates showed considerable variation in their cell size and pigmentation phenotypes, yielding a colorful variety of red, pink and blue-green strains. These pigmentation phenotypes could not be differentiated on the basis of their 16S rRNA-internal transcribed spacer (ITS) sequences. Thirty-nine strains, designated BSea, possessed 16S rRNA-ITS sequences almost identical with Synechococcus strain WH5701. Despite their similar 16S rRNA-ITS sequences, the BSea strains separated into several different clusters when comparing the phycocyanin (cpcBA) operon. This separation was largely consistent with the phycobiliprotein composition of the different BSea strains. The majority of phycocyanin (PC)-rich Bsea strains clustered with WH5701. Remarkably, the phycoerythrin (PE)-rich strains of BSea formed an as yet unidentified cluster within the cpcBA phylogeny, distantly related to other PE-rich groups. Detailed analysis of the cpcBA operon using neighbour-net analysis indicated that the PE-rich BSea strains are probably endemic for the Baltic Sea. Comparison of the phylogenies obtained by the 16S rRNA-ITS, the cpcBA, and the concatenated 16S rRNA-ITS and cpcBA operon sequences revealed possible events of horizontal gene transfer among different Synechococcus lineages. Our results show that microdiversity is important in Synechococcus populations and that it can reflect extensive diversification of different pigmentation phenotypes into different ecological niches.

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Section: Molecular Identification Of the Baltic Sea Picocyanobacteriamentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Molecular Identification Of the Baltic Sea Picocyanobacteriamentioning

confidence: 99%

Section: Molecular Identification Of the Baltic Sea Picocyanobacteriamentioning

confidence: 99%

Section: Pcr Reactionsmentioning

confidence: 99%

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Colorful microdiversity of Synechococcus strains (picocyanobacteria) isolated from the Baltic Sea

et al. 2008

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Performance, genomic rearrangements, and signatures of adaptive evolution: Lessons from fermentative yeasts

Nespolo

Solano-Iguarán

Paleo-López

et al. 2020

Ecology and Evolution

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The capacity of some yeasts to extract energy from single sugars, generating CO2 and ethanol (=fermentation), even in the presence of oxygen, is known as the Crabtree effect. This phenomenon represents an important adaptation as it allowed the utilization of the ecological niche given by modern fruits, an abundant source of food that emerged in the terrestrial environment in the Cretaceous. However, identifying the evolutionary events that triggered fermentative capacity in Crabtree‐positive species is challenging, as microorganisms do not leave fossil evidence. Thus, key innovations should be inferred based only on traits measured under culture conditions. Here, we reanalyzed data from a common garden experiment where several proxies of fermentative capacity were recorded in Crabtree‐positive and Crabtree‐negative species, representing yeast phylogenetic diversity. In particular, we applied the “lasso‐OU” algorithm which detects points of adaptive shifts, using traits that are proxies of fermentative performance. We tested whether multiple events or a single event explains the actual fermentative capacity of yeasts. According to the lasso‐OU procedure, evolutionary changes in the three proxies of fermentative capacity that we considered (i.e., glycerol production, ethanol yield, and respiratory quotient) are consistent with a single evolutionary episode (a whole‐genomic duplication, WGD), instead of a series of small genomic rearrangements. Thus, the WGD appears as the key event behind the diversification of fermentative yeasts, which by increasing gene dosage, and maximized their capacity of energy extraction for exploiting the new ecological niche provided by single sugars.

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Accessory Chlorophyll Proteins in Cyanobacterial Photosystem I

et al.

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Ecosystem-dependent adaptive radiations of picocyanobacteria inferred from 16S rRNA and ITS-1 sequence analysis

Cited by 143 publications

References 57 publications

Colorful microdiversity of Synechococcus strains (picocyanobacteria) isolated from the Baltic Sea

Colorful microdiversity of Synechococcus strains (picocyanobacteria) isolated from the Baltic Sea

Performance, genomic rearrangements, and signatures of adaptive evolution: Lessons from fermentative yeasts

Accessory Chlorophyll Proteins in Cyanobacterial Photosystem I

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