Persimmon-derived tannin ameliorates the pathogenesis of ulcerative colitis in a murine model through inhibition of the inflammatory response and alteration of microbiota

Abstract

Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) induced by dysregulation of the immune response in the intestinal mucosa. Although the underlying mechanisms of UC development are not fully understood, disruption of gut microbiota, “dysbiosis”, is thought to lead to the development of IBD. Persimmon (Ebenaceae Diospyros kaki Thunb.)-derived tannin, which is a condensed polymeric tannin consisting of catechin groups, has antioxidant, anti-inflammatory, and antimicrobial activities. In this study, we assessed the effect of persimmon-derived tannin on a murine model of UC established by dextran sulfate sodium-induced colitis in female mice. Dietary supplementation of tannin significantly decreased disease activity and colon inflammation. A hydrolysate of tannin directly suppressed expression of inflammatory genes in macrophages in vitro. In faecal microbiota, the relative abundance of Bacteroides was increased significantly by tannin supplementation. Alpha-diversity indices in colitis-induced mice were significantly higher in the tannin diet group compared with the control diet group. Additionally, expansion of Enterobacteriaceae and Enterococcus, which is associated with disease progression of IBD, was remarkably suppressed in the tannin diet group. These results suggest that persimmon-derived tannin ameliorates colon inflammation in UC through alteration of the microbiota composition and immune response, which may be a promising candidate for IBD therapy.

Persimmon-derived tannin has antiviral effects and reduces the severity of infection and transmission of SARS-CoV-2 in a Syrian hamster model

“…The procedure for persimmon-derived tannin extraction has been described previously 8 , 9 . Immature persimmon fruits (Ebenaceae Diospyros kaki Thunb., cv.…”

“…The four types of catechin—epicatechin (EC), epicatechin gallate, epigallocatechin, and epigallocatechin gallate (EGCG)—are condensed into chains of 19–47 molecules long 7 . Because of its large molecular size, the persimmon tannin has strong antioxidant, immunomodulatory, and antimicrobial activities 6 , 8 . We have previously shown that dietary intake of the soluble fraction of persimmon-derived tannin attenuated Mycobacterium avium complex (MAC) infection in an in vivo mouse model 9 .…”

Effects of Ginsenoside Rf on dextran sodium sulfate-induced colitis in mice

“…Recent studies reported that female mice were administered DSS in drinking water to induce colitis through alteration of the microbiota composition and immune response, this ability of DSS to alter gut microbiota composition can be targeted for IBD therapy (Kitabatake et al, 2021;Yang et al, 2021). We observed that the use of 5% (w/v) DSS in drinking water for 7 days induced inflammation in the entire colon and led to an increase in the incidences of metaplasia and tumour formation in both rats and mice (Murthy et al, 1993).…”

Antioxidant Potential of Non-Extractable Fractions of Dried Persimmon (Diospyros kaki Thunb.) in Streptozotocin-Induced Diabetic Rats

“…The procedure for persimmon-derived tannin extraction has been described previously [ 23 , 24 , 25 ]. Immature persimmon fruits ( Diospyros kaki Thunb., cv.…”