Persian sturgeon insulin-like growth factor I: molecular cloning and expression during various nutritional conditions

Abstract

The effects of different periods of starvation (1, 2, 3, and 4 weeks) and subsequent re-feeding (over a 4 week) on the compensatory growth performance and insulin-like growth factor I (IGF-I) mRNA expression in liver and white muscle were investigated in juvenile Persian sturgeon (Acipenser persicus). First, a fragment of 617 nucleotides coding for IGF-I was cloned from liver, which included an open reading frame of 486 nucleotides, encoding a 162 amino acid preproIGF-I. This is composed of a 45 aa for signal peptide, a 117 aa for the mature peptide comprising the B, C, A, and D domains, and a 47 aa for E domain. The mature Persian sturgeon IGF-I exhibits high sequence identities with other sturgeon species and teleost, ranging between 68 and 95 %. The pattern of IGF-I mRNA expression in the liver and white muscle was measured in response to different periods of starvation and subsequent re-feeding. Nutritional status influenced IGF-I mRNA expression pattern in both liver and muscle. IGF-I mRNA expression in the liver increased during starvation, before decreasing after re-feeding. Furthermore, white muscle IGF-I mRNA expression showed better responses to nutritional status and decreased following starvation and increased by re-feeding. However, changes in the expression of IGF-I mRNA were not significantly different between any of the treatments in both tissues. These data suggest that muscle and liver IGF-I mRNA expression do not have a regulatory role for somatic growth induced by compensatory growth in Persain sturgeon.

Effects of feeding frequency on juvenile Chinese sturgeon Acipenser sinensis

“…The complete coding region of IGF I from Chinese sturgeon encoded a putative protein composed of 162 amino acids with 45amino acid signal peptide. The result is similar to those of the Persian sturgeon 51 . The complete coding region of IGF II from Chinese sturgeon encoded a putative protein composed of 219 amino acids with no signal peptide.…”

Investigation of insulin-like growth factor-1 gene with egg-laying traits in the Muscovy duck (Cairina moschata)

“…During the past decade, partial or complete sequences of IGF-1 cDNA have been cloned from different animals including the pig (Sus scrofa) (Liu et al 2012), mouse (Mus musculus) (Chu and Best 2003), and Persian sturgeon (Acipenser persicus) (Yarmohammadi et al 2014). It has been proven that feeding IGF-1 to quail can significantly alter the expression levels in muscle tissues (Guo et al 2012).…”

The effects of endosulfan onP4501A gene expression, antioxidant enzymes activity and histopathological alterations in liver of Persian sturgeon (Acipenser persicusBorodin, 1987)

“…DNA in samples was removed by treatment with DNa se I (Fermentas, France). RNA of three samples of each replicate was then pooled (Yarmohammadi et al., ); 1 μ g of total RNA was used to synthesize first‐strand cDNA using a Fermentase cDNA Synthesis kit for RT‐PCR, following the manufacturer's instructions and a mixture of oligo‐ dT as primer.…”

“…Damage to protein and lipid structure of cell membrane can per se cause tissue damage (Glover et al., ). Persian sturgeon ( Acipenser persicus ) is one of the most economically important fishes in the Caspian Sea, in which its stock have declined dramatically mainly due to over fishing, illegal catch, pollution and deterioration of habitats and natural spawning grounds (Pourkazemi et al., ; Yarmohammadi et al., ). Numerous studies have been conducted on histopathological and biochemical effects of some types of pollutant on fishes (Safari et al., ); however, studies on endogenous enzymatic antioxidants and molecular responses to contaminants (toxicogenomic) are limited (Safari et al., ).…”