The effects of endosulfan onP4501A gene expression, antioxidant enzymes activity and histopathological alterations in liver of Persian sturgeon (Acipenser persicusBorodin, 1987)

Abstract

Summary The effects of 14 days exposure to sublethal concentrations of endosulfan (10 and 40 μg L−1) were investigated in mRNA‐ P450 1A expression, antioxidant enzymes (SOD and CAT) activity and histopathological alterations of Persian sturgeon (Acipenser persicus) fingerlings with weights of 3–5 g. The results illustrated that the relative mRNA‐ P450 1A expression level significantly increased (P < 0.05) compared to the control group. Highest significant increase (P < 0.05) was observed on the first day, then decreased towards day 14 of exposure. The SOD and CAT activity showed a significant increase in fish exposed to different concentrations up to day 7, then activity decreased on day 14 in fish of all treated groups. Although signs of tissue lesions were observed on day 4, they increased from day 7 and reached the highest level on day 14. The magnitude of all changed studied parameters (gene expression, enzymes and histopathological) follows a concentration‐dependent manner.

Protective effects of HSP Inducer on diazinon-exposed stellate sturgeon (Acipenser stellatus) fry: Insights on HSP70 gene expression, immune response, and enzyme indices

“…In addition, the expression levels were standardized using the GAPDH gene as a reference gene. The real-time qRT-PCR primers for the HSP70 [ 47 ] and GAPDH [ 48 ] genes are shown in Table 1 . To determine HSP70 gene expression in a 25 μL reaction volume, a Roche qPCR thermocycler (Roche light cycler 96 machines, Germany) was used.…”

The effect of diazinon as agricultural pesticides on reproductive indices and related genes expression in zebrafish ( Danio rerio )

“…Recently, pesticide‐induced deaths in aquatic animals have been reported, leading to a significant environmental concern (Helfrich et al, 2009; Kumari, 2020). The sources of agricultural pesticides in the water comprised of industrial effluents, direct washing, rainfall on sprayed areas, agricultural and urban land, drainage, and irrigation where pesticides are used through the diffuse or via point sources (Safari et al, 2016; Tiryaki & Temur, 2010). Excessive use of these pesticides endangers human health, and non‐target organisms are exposed to these pesticides (Martyniuk et al, 2020; Yen et al, 2011).…”

“…diazinon, gene expression, reproduction, zebrafish on sprayed areas, agricultural and urban land, drainage, and irrigation where pesticides are used through the diffuse or via point sources (Safari et al, 2016;Tiryaki & Temur, 2010). Excessive use of these pesticides endangers human health, and non-target organisms are exposed to these pesticides (Martyniuk et al, 2020;Yen et al, 2011).…”