Peroxiredoxin 1 (Prx1) is a dual-function enzyme by possessing Cys-independent catalase-like activity

Sun, Cen-Cen; Dong, Wei-Ren; Shao, Tong; Li, Jiangyuan; Zhao, Jing; Nie, Li; Xiang, Lei; Zhu, Guangxi; Shao, Jian‐Zhong

doi:10.1042/bcj20160851

Cited by 32 publications

(19 citation statements)

References 55 publications

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“…In addition, the genome harbors the rfbA , rfbB , rfbC , and rfBCD genes, which encode enzymes that are involved in the biosynthesis of dTDP-rhamnose for the assembly of lipopolysaccharide (LPS), suggesting that CBP may have antagonist LPS structures, similar to other Bacteroidetes , such as P. gingivalis and Tannerella [ 32 ] . Two antioxidant enzymes were also identified (a peroxiredoxin, and a 1-Cys-peroxiredoxin), which are known to control cytokine-induced peroxide levels and are thereby mediating signal transduction in mammalian cells [ 33 ]. Furthermore, by performing BLAST analysis of the CBP genome against the well annotated P. gingivalis ATCC 33277 genome, we identified the following shared virulence-associated genes: C25 domains encoding gingipains, which are well-known P. gingivalis proteases that target outer membranes via the Bacteroidetes -specific type 9 secretion system, ragA and ragB surface antigen genes, and hemolysin encoding genes (Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

Discovery of a Novel Periodontal Disease-Associated Bacterium

et al. 2018

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One of the world's most common infectious disease, periodontitis (PD), derives from largely uncharacterized communities of oral bacteria growing as biofilms (a.k.a. plaque) on teeth and gum surfaces in periodontal pockets. Bacteria associated with periodontal disease trigger inflammatory responses in immune cells, which in later stages of the disease cause loss of both soft and hard tissue structures supporting teeth. Thus far, only a handful of bacteria have been characterized as infectious agents of PD. Although deep sequencing technologies, such as whole community shotgun sequencing have the potential to capture a detailed picture of highly complex bacterial communities in any given environment, we still lack major reference genomes for the oral microbiome associated with PD and other diseases. In recent work, by using a combination of supervised machine learning and genome assembly, we identified a genome from a novel member of the Bacteroidetes phylum in periodontal samples. Here, by applying a comparative metagenomics read-classification approach, including 272 metagenomes from various human body sites, and our previously assembled draft genome of the uncultivated Candidatus Bacteroides periocalifornicus (CBP) bacterium, we show CBP's ubiquitous distribution in dental plaque, as well as its strong association with the well-known pathogenic "red complex" that resides in deep periodontal pockets.

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Section: Resultsmentioning

confidence: 99%

Discovery of a Novel Periodontal Disease-Associated Bacterium

et al. 2018

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“…By controlling the activities of TRAF6 and NF-kB; MAST2 regulates immune response and acts as the first responder to harmful cellular stimuli such as stress, free radicals and UV radiation 59 , 60 . While PRDX1 may play an antioxidant protective role in cells, it also contributes to antiviral activity of CD8(+) T-cells 61 , 62 . The ZBP1 activates innate immune response by binding foreign DNA, enhances DNA-mediated induction of type I interferons and other genes that activate innate immune responses, as well as, signaling mechanisms underlying DNA-associated antimicrobial immunity and autoimmune disorders 63 , 64 .…”

Section: Discussionmentioning

confidence: 99%

Genomic footprints of dryland stress adaptation in Egyptian fat-tail sheep and their divergence from East African and western Asia cohorts

Mwacharo¹,

Kim

Elbeltagy

et al. 2017

Sci Rep

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African indigenous sheep are classified as fat-tail, thin-tail and fat-rump hair sheep. The fat-tail are well adapted to dryland environments, but little is known on their genome profiles. We analyzed patterns of genomic variation by genotyping, with the Ovine SNP50K microarray, 394 individuals from five populations of fat-tail sheep from a desert environment in Egypt. Comparative inferences with other East African and western Asia fat-tail and European sheep, reveal at least two phylogeographically distinct genepools of fat-tail sheep in Africa that differ from the European genepool, suggesting separate evolutionary and breeding history. We identified 24 candidate selection sweep regions, spanning 172 potentially novel and known genes, which are enriched with genes underpinning dryland adaptation physiology. In particular, we found selection sweeps spanning genes and/or pathways associated with metabolism; response to stress, ultraviolet radiation, oxidative stress and DNA damage repair; activation of immune response; regulation of reproduction, organ function and development, body size and morphology, skin and hair pigmentation, and keratinization. Our findings provide insights on the complexity of genome architecture regarding dryland stress adaptation in the fat-tail sheep and showcase the indigenous stocks as appropriate genotypes for adaptation planning to sustain livestock production and human livelihoods, under future climates.

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“…The coding sequences for the ORF or the extracellular domain of cd58 and cd2 were amplified through RT-PCR by using primers (shown in Table S1 in Supplementary Material) containing an EcoRI site added to the 5′ end and an XhoI site added to the 3′ end. The PCR products were digested and ligated into pEGFP-N1 (Clontech) or pcDNA6/myc-His©B (Invitrogen) to construct eukaryotic expression vectors (pEGFP- cd58 , pEGFP- cd2 , and pcDNA6- cd58 ) with enhanced GFP-tag or myc-tag and into pMalc2e to construct prokaryotic expression vectors (pMalc2e- cd2 ) with MBP-tag ( 43 ). For eukaryotic expression of Cd58 protein, the plasmid DNA was transformed into HEK293T cells.…”

Section: Methodsmentioning

confidence: 99%

Costimulatory Function of Cd58/Cd2 Interaction in Adaptive Humoral Immunity in a Zebrafish Model

et al. 2018

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CD58 and CD2 have long been known as a pair of reciprocal adhesion molecules involved in the immune modulations of CD8+ T and NK-mediated cellular immunity in humans and several other mammals. However, the functional roles of CD58 and CD2 in CD4+ T-mediated adaptive humoral immunity remain poorly defined. Moreover, the current functional observations of CD58 and CD2 were mainly acquired from in vitro assays, and in vivo investigation is greatly limited due to the absence of a Cd58 homology in murine models. In this study, we identified cd58 and cd2 homologs from the model species zebrafish (Danio rerio). These two molecules share conserved structural features to their mammalian counterparts. Functionally, cd58 and cd2 were significantly upregulated on antigen-presenting cells and Cd4+ T cells upon antigen stimulation. Blockade or knockdown of Cd58 and Cd2 dramatically impaired the activation of antigen-specific Cd4+ T and mIgM+ B cells, followed by the inhibition of antibody production and host defense against bacterial infections. These results indicate that CD58/CD2 interaction was required for the full activation of CD4+ T-mediated adaptive humoral immunity. The interaction of Cd58 with Cd2 was confirmed by co-immunoprecipitation and functional competitive assays by introducing a soluble Cd2 protein. This study highlights a new costimulatory mechanism underlying the regulatory network of adaptive immunity and makes zebrafish an attractive model organism for the investigation of CD58/CD2-mediated immunology and disorders. It also provides a cross-species understanding of the evolutionary history of costimulatory signals from fish to mammals as a whole.

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Peroxiredoxin 1 (Prx1) is a dual-function enzyme by possessing Cys-independent catalase-like activity

Cited by 32 publications

References 55 publications

Discovery of a Novel Periodontal Disease-Associated Bacterium

Discovery of a Novel Periodontal Disease-Associated Bacterium

Genomic footprints of dryland stress adaptation in Egyptian fat-tail sheep and their divergence from East African and western Asia cohorts

Costimulatory Function of Cd58/Cd2 Interaction in Adaptive Humoral Immunity in a Zebrafish Model

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