Peroxidase-Like Catalytic Activity of Metal Complexes of Thiacalix[4]arenetetrasulfonate on the Modified Ion-Exchanger and Its Application to the Determination of Hydrogen Peroxide1

Abstract

Calix [n]arenes are a class of macrocycles that have attracted much interest because of their potential for forming host-guest complexes, and have been extensively investigated in various fields. In this study, in an attempt to develop calix[n]arenes as an effective analytical reagent with enzyme-like activity, the peroxidase-like activity of the ion-exchangers modified with some metal complexes of thiacalix[4]arenetetrasulfonate (Fig. 1) was investigated. The modified ion-exchanger with the highest activity was applied for the determination of hydrogen peroxide and glucose in place of peroxidase.

Determination of Hydrogen Peroxide by Iron(III) Complex of Thiacalix[4]arenetetrasulfonate on a Modified Ion-Exchanger with Peroxidase-like Catalytic Activity1

“…Figure 3 shows the absorbances at 582 nm of the quinoid dye produced through color reaction (2) by Fe 3+ -TCASA-500 at each pH. In the previous method, 19 Fe 3+ -TCASA-500 exhibited high activity in alkaline buffer solutions (pH 10 -11) for color reaction (1) using 4-AAP and phenol. However, for color reaction (2) in this study, Fe 3+ -TCASA-500 exhibited high activity in acidic buffer solutions, especially at pH 5 -6.…”

“…In the previous method, 19 the reaction solution was incubated for 60 min for the maximum and constant absorbance of the quinoid dye in color reaction (1). However, in the method using the MBTH-HALPS system the absorbances at 582 nm became almost maximum and constant above 15 min of incubation time, as shown in Fig.…”

“…appreciably higher activity for color reaction (2) using MBTH and HALPS than that for color reaction (1) using 4-AAP and phenol in the previous method. 19 …”

“…A previous method using Fe 3+ -TCASA-500, 19 which we recently developed for determining H2O2 and glucose in blood, still has some disadvantages regarding its sensitivity and reaction conditions; its sensitivity is still not sufficient (apparent molar absorptivity: 3 × 10 3 l mol -1 cm -1 ), and its reaction solution must be adjusted to be strongly alkaline (pH 10 -11) and to be incubated for a relatively long time (60 min). In this study, in An iron(III) complex of thiacalix [4]arenetetrasulfonate on a modified anion-exchanger (Fe 3+ -TCASA-500) has shown high peroxidase-like activity at pH 5 -6 for the reaction of quinoid-dye formation between 3-methyl-2-benzothiazolinone hydrazone and N- (3-sulfopropyl)aniline in the presence of hydrogen peroxide.…”

“…This determination method of hydrogen peroxide using Fe 3+ -TCASA-500 was applied for the determination of glucose in diluted normal and abnormal control serum I and II. an attempt to improve especially the low sensitivity of the method using Fe 3+ -TCASA-500, the activity of Fe 3+ -TCASA-500 for the color reaction (2) between 3-methyl-2-benzothiazolinone hydrazone (MBTH) and N- (3-sulfopropyl)aniline (HALPS) in the presence of H2O2, instead of color reaction (1) between 4-aminoantipyrine (4-AAP) and phenol used in the previous method, 19 was investigated. Moreover, the activity of Fe 3+ -TCASA-500 was applied to the determination of H2O2 and serum glucose using color reaction (2).…”