Peroxidase-like activity of nanocrystalline cobalt selenide and its application for uric acid detection

Abstract

Dendrite-like cobalt selenide nanostructures were synthesized from cobalt and selenium powder precursors by a solvothermal method in anhydrous ethylenediamine. The as-prepared nanocrystalline cobalt selenide was found to possess peroxidase-like activity that could catalyze the reaction of peroxidase substrates in the presence of H 2 O 2 . A spectrophotometric method for uric acid (UA) determination was developed based on the nanocrystalline cobalt selenide-catalyzed coupling reaction between N -ethyl- N -(3-sulfopropyl)-3-methylaniline sodium salt and 4-aminoantipyrine (4-AAP) in the presence of H 2 O 2 . Under optimum conditions, the absorbance was proportional to the concentration of UA over the range of 2.0–40 μM with a detection limit of 0.5 μM. The applicability of the proposed method has been validated by determination of UA in human serum samples with satisfactory results.

Peroxidase-Like Behavior of Ni Thin Films Deposited by Glancing Angle Deposition for Enzyme-Free Uric Acid Sensing

“…15−17 Nanoparticles used as "artificial enzymes" or "nanozymes" have also been investigated, and they have been shown to possess remarkably improved stability under harsh working conditions, and well as efficient catalytic activity at low concentrations. 18,19 For instance, Kumar et al 3 used gold nanoparticles as a catalyst to accelerate the TMB oxidation reaction for UA sensing, and Kumar et al 5 expanded the catalytic surface area and reduced the limit of detection (LOD) by incorporating graphene oxide. These studies suggest that the extended surface area of the catalyst plays an important role in increasing the absorbance of oxTMB.…”

“…Throughout the literature, the initial oxidation of TMB is generally done through a natural peroxidase-based catalyst; however, these natural enzymes often require well-controlled storage conditions (temperature, pH, purity, etc. ), which may result in poor stability and shelf-life. − Nanoparticles used as “artificial enzymes” or “nanozymes” have also been investigated, and they have been shown to possess remarkably improved stability under harsh working conditions, and well as efficient catalytic activity at low concentrations. , For instance, Kumar et al used gold nanoparticles as a catalyst to accelerate the TMB oxidation reaction for UA sensing, and Kumar et al expanded the catalytic surface area and reduced the limit of detection (LOD) by incorporating graphene oxide. These studies suggest that the extended surface area of the catalyst plays an important role in increasing the absorbance of oxTMB.…”

“…are also being explored as peroxidase mimics, and some of these high-surface-area catalysts have been used for the colorimetric detection of UA. For example, Liu et al used peroxidase-mimicking MOFs in the presence of TMB to colorimetrically detect H 2 O 2 generated during the reaction of uric acid with uricase, and other nanoparticles and nanocrystals have also been used to mimic the behavior of peroxidase for UA biosensing. ,− Among these high-surface-area nanozymes, Ni has begun to attract considerable attention due to its excellent catalytic activity, high stability, low cost, and ease of preparation . One common drawback among these nanomaterial-based systems, however, is the difficulty in recovering the catalyst after use, which prevents the reuse of nanozyme catalysts. , …”

Ultrasensitive detection of uric acid in serum of patients with gout by a new assay based on Pt@Ag nanoflowers

“…The other one is the catalytic oxidation strategy, which bases on the peroxidase-like properties of nanomaterials, such as porous metal–organic framework MIL-53 (Fe), 21 graphite carbonitride nanosheets 22 and cobalt selenide nanosheets. 23 They can change color by H 2 O 2 catalytic peroxidase substrate. The peroxidase mimic enzymes have the advantages of low cost, good stability, simple preparation, controllable structure and composition, and adjustable catalytic activity.…”

A comprehensive review on thin film-based nano-biosensor for uric acid determination: arthritis diagnosis

“…In 1942 (Movlaee et al, 2017), Bulger and Johns introduced a method based on the use of uricase enzyme though it is commercially available its application is limited due to the cost of uricase enzyme. Alternative methods such as chemiluminescence (Wu et al, 2006), fluorescence (Erden and Kilic, 2013;Afrasiabi et al, 2016), spectrophotometry (Zhao et al, 2009), HPLC-mass spectrometry (Weiss and Lunte, 2000), ion-chromatography (Revin and John, 2012), high performance liquid chromatography and calorimetry (Zhuang et al, 2017) have been reported. However these methods are usually expensive, laborious and complex to perform.…”