Ultrasensitive detection of uric acid in serum of patients with gout by a new assay based on Pt@Ag nanoflowers

Wang, Xue; Chen, Shujun; Tang, Xiaomin; Lin, Daiqin; Qiu, Ping

doi:10.1039/c9ra06481h

Cited by 24 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the sensing assay, iodide was used as a replacement for the conventional horseradish peroxidase used in colorimetric assays because it is a common and inexpensive reagent and exhibits peroxidase-like activity [ 27 , 28 ], which makes it an ideal peroxidase mimetic. Catalyzed by uricase, UA generated H 2 O 2 after being oxidized to 5-hydroxyisourate [ 29 ], which was converted to hydroxyl radicals ( • OH) in the presence of iodide ions. The • OH species then oxidized o -phenylenediamine (OPD) to produce 2,3-diaminophenazine (DAP), which produced yellow fluorescence emission at 580 nm and quenched the fluorescence emission of the N-CDs at 427 nm based on the inner filter effect (IFE).…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Rui

Yan

et al. 2021

Microchim Acta

Self Cite

View full text Add to dashboard Cite

Graphical abstract A simple dual-read assay for uric acid (UA) was developed based on a combined ratiometric fluorescent and colorimetric strategy using nitrogen-doped carbon dots (N-CDs). The biosensor relies on the oxidation of UA by uricase to produce H 2 O 2 , which was then converted to • OH radicals by I - , resulting in the oxidation of o -phenylenediamine (OPD) to 2,3-diaminophenazine (DAP). In the presence of UA, the colorless biosensor system changed to yellow. Furthermore, the presence of DAP quenched the fluorescence emission of the N-CDs at 427 nm based on the inner filter effect (IFE). With increasing UA concentrations, the fluorescence intensity of the biosensor at 427 nm decreased but increased at 580 nm, demonstrating the ratiometric response. A strong linearity was observed between the fluorescence intensity ratio of DAP to N-CDs ( I 580 / I 427 ) and the corresponding UA concentration over the range 0.5−150 μM, and a limit of detection (S/N ratio of 3) of 0.06 μM was calculated. The dual-read assay was successfully employed in the quantitation of UA in human serum and urine samples, revealing its potential for measuring UA in clinical samples. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04971-2.

show abstract

Section: Introductionmentioning

confidence: 99%

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Rui

Yan

et al. 2021

Microchim Acta

Self Cite

View full text Add to dashboard Cite

show abstract

“…The determination of uric acid in serum can be accomplished using numerous approaches, such as capillary electrophoresis, fluorometry, chromatography, electrochemical methods, chemiluminescence, and colorimetry. The colorimetric method is the most widely used due to its ease of use, high analysis speed, and high sensitivity [ 84 ]. In healthy adults, uric acid must be less than 6.6 mg/dL or 360 µmol/L [ 85 ].…”

Section: Biomarkers Predictive Of Metabolic Syndrome and Cardiovascul...mentioning

confidence: 99%

Biomarkers Predictive of Metabolic Syndrome and Cardiovascular Disease in Childhood Cancer Survivors

Romano

Vescovo

Rivetti

et al. 2022

JPM

View full text Add to dashboard Cite

The improvement in childhood cancer treatments resulted in a marked improvement in the survival of pediatric cancer patients. However, as survival increased, it was also possible to observe the long-term side effects of cancer therapies. Among these, metabolic syndrome is one of the most frequent long-term side effects, and causes high mortality and morbidity. Consequently, it is necessary to identify strategies that allow for early diagnosis. In this review, the pathogenetic mechanisms of metabolic syndrome and the potential new biomarkers that can facilitate its diagnosis in survivors of pediatric tumors are analyzed.

show abstract

“…The method was adapted from the literature [19] as described below: to 10 mL solution CuNPs (3.22 × 10 −3 M) were added 10 mL solution H 2 PtCl 6 × 6H 2 O (c = 5.6 × 10 −3 M), so that a mole ratio of CuNPs/H 2 PtCl 6 × 6H 2 O = 1:1.75 was achieved. The mixture was stirred at room temperature for 20 min until the color turned dark-grey.…”

Section: Obtaining Of Mixed Nanoparticles With Cu Core and Pt Shell Pt@cunpsmentioning

confidence: 99%

“…Using UV-Vis spectrometry, different concentrations (0.8 mg/mL, 0.6 mg/mL, 0.4 mg/mL) of uric acid were precisely detected at a constant absorption UV wavelength, 294.46 nm in a short time response [17,18]. A colorimetric assay using Pt@Agnanoflowers (Pt@AgNFs) was developed to detect UA with high sensitivity [19]. The color of the solution changes from colorless to blue, depending on the amount of UA.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Uric Acid Optical Detection Using as Sensitive Materials an Amino-Substituted Porphyrin and Its Nanomaterials with CuNPs, PtNPs and Pt@CuNPs

et al. 2021

View full text Add to dashboard Cite

Hybrid nanomaterials consisting in 5,10,15,20-tetrakis(4-amino-phenyl)-porphyrin (TAmPP) and copper nanoparticles (CuNPs), platinum nanoparticles (PtNPs), or both types (Pt@CuNPs) were obtained and tested for their capacity to optically detect uric acid from solutions. The introduction of diverse metal nanoparticles into the hybrid material proved their capacity to improve the detection range. The detection was monitored by using UV-Vis spectrophotometry, and differences between morphology of the materials were performed using atomic force microscopy (AFM). The hybrid material formed between porphyrin and PtNPs hasthe best and most stable response for uric acid detection in the range of 6.1958 × 10−6–1.5763 × 10−5 M, even in the presence of very high concentrations of the interference species present in human environment.

show abstract

Ultrasensitive detection of uric acid in serum of patients with gout by a new assay based on Pt@Ag nanoflowers

Abstract: A ultrasensitive assay for the determination of uric acid (UA) based on Pt@Ag nanoflowers (Pt@Ag NFs) was constructed.

Cited by 24 publications

References 45 publications

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Biomarkers Predictive of Metabolic Syndrome and Cardiovascular Disease in Childhood Cancer Survivors

A Comparison of Uric Acid Optical Detection Using as Sensitive Materials an Amino-Substituted Porphyrin and Its Nanomaterials with CuNPs, PtNPs and Pt@CuNPs

Contact Info

Product

Resources

About