Peroxidase Isozymes from Horseradish Roots

Shannon, Leland M.; Kay, Ernest; Lew, J.

doi:10.1016/s0021-9258(18)96680-9

Cited by 863 publications

(97 citation statements)

References 23 publications

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“…The similarity of the carbohydrate composition of many plant and animal glycoproteins has been noted (Sharon & Lis, 1972). Both N-acetylglucosamine and galactose are frequent constituents, but no monosaccharide sequences have been reported (Shannon et al, 1966;Ericson & Chrispeels, 1973).…”

Section: Discussionmentioning

confidence: 99%

Glycosyltransferases in the Golgi membranes of onion stem

Powell

Brew

1974

Biochemical Journal

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Cell fractions consisting largely of Golgi membranes were prepared from the meristematic region of the onion. Several enzyme activities were found to be localized in these fractions: inosine diphosphatase, galactosyltransferases and glucosyltransferases. The fractions catalysed the transfer of [(14)C]galactose from UDP-galactose to endogenous and cell-sap acceptors, to N-acetylglucosamine and to ovalbumin. In the presence of bovine alpha-lactalbumin, transfer to glucose (lactose synthesis) was catalysed. [(14)C]Glucose was transferred from UDP-glucose to endogenous and cell-sap acceptors, to cellobiose and to fructose (sucrose synthesis). All these activities were latent, being potentiated by detergents (Triton X-100 or sodium deoxycholate). The characteristics of some of these enzyme activities are described and their biological significance is discussed.

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Section: Discussionmentioning

confidence: 99%

Glycosyltransferases in the Golgi membranes of onion stem

Powell

Brew

1974

Biochemical Journal

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“…Bovine serum albumin (92%) was used as a standard to quantify total protein content of the samples. The POD enzyme activity was determined using the method of Shannon et al (1966) measuring absorbance change at 480 nm and regarding 0.01 ΔA min -1 as 1 Unit. The PPO enzyme activity was assayed according to the method of Flurkey & Jen (1978) at 420 nm, taking 0.001 ΔA min -1 as 1 Unit.…”

Section: Enzyme Activity Analysismentioning

confidence: 99%

Tracing the acclimation of European beech (Fagus sylvatica L.) populations to climatic stress by analyzing the antioxidant system

Visi-Rajczi¹,

Hofmann²,

Albert³

et al. 2021

iForest

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Biogeosciences and Forestry Biogeosciences and ForestryTracing the acclimation of European beech (Fagus sylvatica L.) populations to climatic stress by analyzing the antioxidant system Eszter Visi-Rajczi (1) , Tamás Hofmann (1) , Levente Albert (1) , Csaba Mátyás (2) Through a common garden (provenance) experiment, we investigated the metabolic responses to climatic stress with regard to the acclimation potential of different European beech (Fagus sylvatica L.) populations. Selected enzymatic and non-enzymatic antioxidants were analyzed in leaves. Peroxidase (POD) and polyphenol oxidase (PPO) enzyme activity, total protein content as well as ABTS [2,2'-azino-bis-(3-etylbenzothiazoline)-6-sulphonic acid] antioxidant capacity were measured in the leaves of selected populations. Major leaf polyphenols were identified and their relative amounts were compared. Significant correlations were found between phenotypic (diameter growth) response to simulated climatic stress and the activity (and amount) of selected chemical components. The concentrations of certain polyphenols, POD enzyme activity, and total protein content may be chemical indicators of the acclimation potential of populations and may contribute to the forecasting of climate change effects, which can aid in the selection of suitable propagation material for adaptive silviculture.

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“…The enzyme was further puriÐed using a reported method. 24 The enzyme was dissolved in 5 mM acetate bu †er at pH 4.4 and applied to a CM52 column equilibrated with the same bu †er. The bound protein was washed thoroughly with 5 mM acetate bu †er at pH 4.4 and eluted by 100 mM acetate bu †er at pH 4.4.…”

Section: Notes and Referencesmentioning

confidence: 99%

Direct electrochemical oxidation of horseradish peroxidase: cyclic voltammetric and spectroelectrochemical studies

Chattopadhyay¹,

Mazumdar²

1999

New J. Chem.

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The direct, unmediated electrochemistry of horseradish peroxidase (HRP) has been studied using a tin doped indium oxide coated glass working electrode at ambient pH. A well deÐned quasi-reversible cyclic voltammogram was obtained at high positive potentials corresponding to an of + 700 mV E 1¿2 ¿s. NHE. The cyanide complex of HRP also showed similar values of indicating formation of a pophyrin centred cation E 1¿2 , radical upon oxidation of the enzyme.

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Peroxidase Isozymes from Horseradish Roots

Cited by 863 publications

References 23 publications

Glycosyltransferases in the Golgi membranes of onion stem

Glycosyltransferases in the Golgi membranes of onion stem

Tracing the acclimation of European beech (Fagus sylvatica L.) populations to climatic stress by analyzing the antioxidant system

Direct electrochemical oxidation of horseradish peroxidase: cyclic voltammetric and spectroelectrochemical studies

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