Peroxidase-catalysed rupture of the CF bond as and indicator reaction for enzyme immunoassay

Brochot, Jean; Siddiqi, I.

doi:10.1016/s0003-2670(00)86569-1

Cited by 20 publications

(7 citation statements)

References 7 publications

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“…The gallium(III) porphyrins display the largest selectivity in liquid/polymer membrane electrodes for fluoride over chloride, which may provide an alternative for fluoride detection. While the classical Orion fluoride ISE, based on a single crystal of lanthanum fluoride, is still the preferred method to determine fluoride concentration, with further improvements in F -selectivity via changes in porphyrin structures, gallium(III) porphyrinbased ISEs could offer practical advantages in more specialized applications such as modifiable-detectors of enzyme-linked immunoassay (ELISA) techniques 41 , optical sensors 42 , or microfabricated sensor arrays. Membrane electrodes doped with thallium(III) porphyrins provide increased selectivity for chloride when compared to indium(III) counterparts and also exhibit near-Nernstian responses when anionic sites are added to the membrane formulation.…”

Section: Resultsmentioning

confidence: 99%

Response Characteristics of Anion-Selective Polymer Membrane Electrodes Based on Gallium(III), Indium(III) and Thallium(III) Porphyrins

1998

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With the evolution of ion-selective ionophore-based liquid/polymer membrane electrodes (ISEs) over the past 25 years, many cations of physiological and industrial significance can be measured effectively by direct potentiometry. However, there is a noticeable lack of analogous electrodes for many common anions. 1 Although commercial electrodes based on anionexchangers such as quaternary ammonium salts can be analytically useful, their selectivity patterns are always correlated solely with anion lipophilicity, resulting in the classical Hofmeister series (ClO 4 ->SCN -> salicyl a t eTherefore, highly hydrated anions such as fluoride, bicarbonate, chloride, and nitrite are difficult to monitor due to significant interference from more lipophilic anion species that may be present in the sample. Recently, examination of a variety of compounds that have strong yet reversible interactions with target anions has resulted in new ionophores with decidedly non-Hofmeister selectivity toward anions. 3,4 Many research groups are studying a wide variety of new ionophores that demonstrate these characteristics such as mercury 5 , silver 6 , and tin 7 organometallic compounds, fluorinated (poly)ethers 8 , metallophthalocyanines 9 , metallocenes 10 , and trifluoroacetyl derivatives. 11 The pioneering work of Simon et al., in which the behavior of lipophilic vitamin B 12 derivatives as ionophores was examined, led to one of the first nitrite selective liquid/polymer membrane electrodes. 12 Metalloporphyrins, which are one carbon larger but structurally analogous to the cobyrinate ring of vitamin B 12 , also exhibit unique anion ionophore properties when incorporated into plasticized polymer membranes. Mn(III) [13][14][15][16][17][18][19][20] , Co(III) 13,16,[21][22][23][24][25][26] , Ru(II) 16 , and Sn(IV) 25,27-29 porphyrin-based ISEs have been shown to exhibit useful selectivity for thiocyanate, nitrite/thiocyanate, thiocyanate, and salicylate, respectively. More recently, the nature of the ionophore-anion interaction mechanism has been correlated to the charge of the central metal ion.25 Metal(II) porphyrins were found to serve as neutral carriers, metal(IV) as charged carriers and metal(III) porphyrins as either neutral or charged depending on the existance and number of bound axial ligands. The use of lipophilic ionic additives, either cationic (quaternary ammonium salts for neutral carriers) or anionic (tetraphenylborate salts for charged carriers) can help to determine the dominant mechanism within the polymer membrane responsible for EMF response. 30 These ionic sites have also been found to improve general electrode performance by reducing the membrane resistance, decreasing counter-ion interference, and enhancing selectivity. 25,[30][31][32] In this work, porphyrins containing group XIII ions as metal centers are examined as unique ionophores in the preparation of anion ISEs. Electrodes based on indium(III) porphyrins in poly(vinyl chloride) (PVC) membranes have been shown previously to exhibit enhanced responses toward...

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Section: Resultsmentioning

confidence: 99%

Response Characteristics of Anion-Selective Polymer Membrane Electrodes Based on Gallium(III), Indium(III) and Thallium(III) Porphyrins

1998

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“…Therefore, it is necessary to develop sensitive and selective methods for the detection of AFP. Several conventional immunological methods, such as radioimmunoassay (RIA) [23], enzymelinked immunosorbent assay (ELISA) [24], chemiluminescent enzyme immunoassay (CLEIA) [25] are usually used for AFP detection. These methods are very sensitive with low limit of detection, for example, the detection limit for RIA is 0.01 nmol L…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Electrochemical Immunosensor for α‐Fetoprotein Detection with Colloidal Gold‐Based Dentritical Enzyme Complex Amplification

Liu

Zheng

et al. 2010

Electroanalysis

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A sensitive and specific electrochemical immunosensor was developed with a-fetoprotein (AFP) as the model analyte by using gold nanoparticle label for enzymatic catalytic amplification. A self-assembled monolayer membrane of mercaptopropionic acid (MPA) was firstly formed on the electrode surface through gold-sulfur interaction. Monoclonal mouse anti-human AFP was covalently immobilized to serve as the capture antibody. In the presence of the target human AFP, gold nanoparticles coated with polyclonal rabbit anti-human AFP were bound to the electrode via the formation of a sandwiched complex. With the introduction of goat anti-rabbit IgG conjugated with alkaline phosphatase, the dentritical enzyme complex was formed through selective interaction of the secondary antibodies with the colloidal gold-based primary antibody at the electrode, thus affording the possibility of signal amplification for AFP detection. Current response arising from the oxidation of enzymatic product was significantly amplified by the dentritical enzyme complex. The current signal was proportional to the concentration of AFP from 1.0 ng mL À1 to 500 ng mL À1 with a detection limit of 0.8 ng mL À1. This system could be extended to detect other target molecules with the corresponding antibody pairs.

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“…Thus, developing a rapid and sensitive detection method for AFP is of great importance in clinical research. Many conventional methods, including enzyme-linked immunosorbent assay (ELISA) [7], electrochemistry [8], electrochemiluminescence (ECL) [9], mass spectrometry [10], quartz crystal microbalance (QCM) [11], and surface plasmon resonance (SPR) immunoassays [12], have been reported for the detection of AFP. Except the high accuracy, some of these techniques involve the disadvantages such as relatively sophisticated instruments, significant sample volume, limited sensitivity, and clinically unrealistic expense and long detection time.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive photoelectrochemical immunoassay through tag induced exciton trapping

Wen

2015

Talanta

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Peroxidase-catalysed rupture of the CF bond as and indicator reaction for enzyme immunoassay

Cited by 20 publications

References 7 publications

Response Characteristics of Anion-Selective Polymer Membrane Electrodes Based on Gallium(III), Indium(III) and Thallium(III) Porphyrins

Response Characteristics of Anion-Selective Polymer Membrane Electrodes Based on Gallium(III), Indium(III) and Thallium(III) Porphyrins

A Sensitive Electrochemical Immunosensor for α‐Fetoprotein Detection with Colloidal Gold‐Based Dentritical Enzyme Complex Amplification

Ultrasensitive photoelectrochemical immunoassay through tag induced exciton trapping

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