Ultrasensitive photoelectrochemical immunoassay through tag induced exciton trapping

Wen, Guangming; Ju, Huangxian

doi:10.1016/j.talanta.2014.11.041

Cited by 31 publications

(10 citation statements)

References 35 publications

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“…Although some results were obtained, sophisticated instruments, significant sample volume, limited sensitivity, and long detection time limited the clinical application13. To develop the clinical detection, a novel, highly sensitive and alternative detection method of AFP is desired.…”

mentioning

confidence: 99%

Photoelectrochemical detection of alpha-fetoprotein based on ZnO inverse opals structure electrodes modified by Ag2S nanoparticles

Jiang

Liu

Yang

et al. 2016

Sci Rep

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In this work, a new photoelectrochemical biosensor based on Ag2S nanoparticles (NPs) modified macroporous ZnO inverse opals structure (IOs) was developed for sensitive and rapid detection of alpha fetal protein (AFP). Small size and uniformly dispersed Ag2S NPs were prepared using the Successive Ionic Layer Adsorption And Reaction (SILAR) method, which were adsorbed on ZnO IOs surface and frame work as matrix for immobilization of AFP. The composite structure of ZnO/Ag2S expanded the scope of light absorption to long wavelength, which can make full use of the light energy. Meanwhile, an effective matching of energy levels between the conduction bands of Ag2S and ZnO are beneficial to the photo-generated electrons transfer. The biosensors based on FTO (fluorine-doped tinoxide) ZnO/Ag2S electrode showed enough sensitivity and a wide linear range from 0.05 ng/mL to 200 ng/mL with a low detection limit of 8 pg/mL for the detection of AFP. It also exhibited high reproducibility, specificity and stability. The proposed method was potentially attractive for achieving excellent photoelectrochemical biosensor for detection of other proteins.

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mentioning

confidence: 99%

Photoelectrochemical detection of alpha-fetoprotein based on ZnO inverse opals structure electrodes modified by Ag2S nanoparticles

Jiang

Liu

Yang

et al. 2016

Sci Rep

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“…Simultaneously, due to abundant carboxyl group active sites, GO in the AC 60 -Gr-GO nanohybrid guaranteed sufficient aqueous dispersity and a friendly anchor for facile bioconjugation. As an example, the AC 60 -Gr-GO based PEC immunosensor (Figure ) had a broad dynamic detection range from 1 pg·mL –1 to 100 ng·mL –1 and a detection limit of as low as 0.54 pg·mL –1 in sensing alpha-fetoprotein (AFP), which were superior/competitive to PEC immunosensors for AFP in previous reports. − …”

mentioning

confidence: 82%

Metal-Free All-Carbon Nanohybrid for Ultrasensitive Photoelectrochemical Immunosensing of alpha-Fetoprotein

et al. 2018

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C can accept up to six electrons reversibly and show exceptional light absorption over the entire UV-vis spectrum, making it a potential photoactive probe for photoelectrochemical (PEC) bioassay. However, few successful works have been reported to apply fullerenes in PEC biosensing, partially because of the low electronic conductivity and poor interfacial interactions with targeted biomolecules. Herein, we report the addressing of these two obstacles by coupling high conductive graphite flake (Gr), graphene oxide (GO) with sufficient oxygen-containing functional groups, and an alkylated C (AC) into a metal-free all-carbon nanohybrid (AC-Gr-GO) via harnessing delicate noncovalent interactions among them through a facile mechanical grinding. It was revealed that the as-obtained AC-Gr-GO nanohybrid not only showed conspicuous enhancement of photocurrent up to 35 times but also offered rich anchors for bioconjugation. With detection of alpha-fetoprotein as an example, the AC-Gr-GO based PEC immunosensor demonstrated a broad linear detection range (1 pg·mL to 100 ng·mL) and a detection limit as low as 0.54 pg·mL, superior/competitive to PEC immunosensors for AFP in previous reports. By a proper reinforcement in conductivity and biointerface engineering, this work may provide a new way to use fullerenes as photoactive materials in more general PEC biosensing.

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“…The detection limit was low enough to measure AFP in human sera. The detection limit of 0.56 pg•mL -1 (S/N = 3) was lower than 38 pg•mL -1 of CdTe QDs modified electrode [42] and 20 pg•mL -1 of CdS/TiO 2 modified electrode [43]. Table 1 presented comparison of the results derived from the proposed immunosensor and those of other teams in details.…”

Section: Fabrication Of Au-zno Flower-rod Heterostructure Electrodementioning

confidence: 95%

A photoelectrochemical immunosensor for detection of α-fetoprotein based on Au-ZnO flower-rod heterostructures

Han

Luo

Chen

et al. 2017

Applied Surface Science

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In this work, a novel label free photoelectrochemical (PEC) immunosensor has been developed for the detection of α-fetoprotein (AFP). The immunosensor was based on Au-ZnO flower-rods (FRs) heterostructure, where Au nanoparticles (NPs) were firstly electrodeposited by cyclic voltammetry methods. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Mott-Schottky plot (MS), UV-vis diffuse reflectance spectrum and fluorescence emission spectrum were used for the characterizations of Au-ZnO FRs. The results demonstrated that Au NPs not only obviously enhanced the visible light absorption of ZnO FRs due to surface plasmon resonance (SPR) but also improved the separation of photo-generated electron-hole pairs. Therefore, the photocurrent of Au-ZnO FRs was increased under simulated sunlight. The photocurrent was reduced after the specific antibody-antigen immune reaction. And the photocurrent decrement was linear with the logarithm of AFP antigen concentration in the range from 0.005 ng•mL-1 to 50 ng•mL-1 with a low detection limit of 0.56 pg•mL-1 (S/N = 3). The PEC immunosensor also exhibited high anti-interference property and acceptable stability. This work would provide a promising photoelectrochemical strategy for the detection of other proteins in clinical diagnosis.

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Ultrasensitive photoelectrochemical immunoassay through tag induced exciton trapping

Cited by 31 publications

References 35 publications

Photoelectrochemical detection of alpha-fetoprotein based on ZnO inverse opals structure electrodes modified by Ag2S nanoparticles

Photoelectrochemical detection of alpha-fetoprotein based on ZnO inverse opals structure electrodes modified by Ag2S nanoparticles

Metal-Free All-Carbon Nanohybrid for Ultrasensitive Photoelectrochemical Immunosensing of alpha-Fetoprotein

A photoelectrochemical immunosensor for detection of α-fetoprotein based on Au-ZnO flower-rod heterostructures

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